The kinetic parameters of a 5-day growth of sarcoma 180 (ascites) have been determined, using both the labelled mitosis method after pulse labelling with 3H thymidine and the 3H and 14C thymidine double labelling technic. The two methods yielded reproducible results, labelling index 73 %; duration of the period of DNA synthesis (S phase) 13 hours (13.5 from double labelling: technic mitosis 0.24 (0.34) hours; G1 + G2 5.3 (4.76) hours; Te 18.5 (18.6) hours. The growth fraction obtained after repeated 3H thymidine injections was 0.95 and as calculated from the other kinetic parameters 0.98.
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References
1.
DefendiV., and MansonL. A.: Analysis of the life cycle in mammalian cells.Nature,198: 359–363, 1963.
2.
EvensJ. S., MusserE. A., BostwickL., and MengelG. D.: The effect of 1-B-Arabinofuranosylcytosine hydrochloride in murine neoplasms.Cancer Res.,24: 1285–1293, 1964.
3.
GaetaniM., SilvestriniR., e BelliniO.: La proliferazione cellulare dell'epatoma ascite AH 130 di Yoshida.Lo sperimentale,114: 293–304, 1964.
4.
JohnsonH. A.: Some problems associated with the histological study of cell proliferation.Kinetics Cytologia (Tokyo),26: 32–41, 1961.
5.
LooneyW. B., MayoA. A., JannesM. Y., MellonJ. G., AllenP., SalakD., and MorrisH. P.: Cell proliferation and tumor growth in hepatomas 3924 A.Cancer Res.,31: 821–825, 1971.
6.
MendelshonM. L., DohanF. C., and MooreH. A.: Autoradiographic analysis of cell proliferation in spontaneous breast cancer of C3H mouse. I. Typical cell cycle and timing of DNA synthesis.J. Nat. Cancer Inst.,25: 476–484, 1960.
7.
MihicE., MulhernA. I., and HornungN.: Inhibiting effect of decamethylenediguanidine (Synthalin) against leukemia L 1210 and sarcoma 180 (ascites).Cancer Res.,20: 609–612, 1960.
8.
NishiokaB.: Proliferation kinetics of sarcoma 180.Gann,61: 563–568, 1970.
9.
PolvaniF., e SilvestriniR.: Note di tecnica autoradiografica con pellicole « stripping ».Riv. Istoch. Norm. Patol.,2: 239–256, 1956.
10.
QuastlerH., and ShermanF. G.: Cell population kinetics in the intestinal epithelium of the mouse.Exp. Cell Res.,17: 420–438, 1959.
11.
SilvestriniR., e LenazL.: Importanza della conoscenza dei parametri cinetici e biochimici per la formulazione degli schemi di trattamento chemioterapici.Tumori,57: 222–223, 1971.
12.
SkipperH. E., SchabelF. M.Jr., and WilcoxW. S.: Experimental evaluation of potential anticancer agents. XXI. Scheduling of arabinosylcytosine to take advantage of its S-phase specificity against leukemic cells.Cancer Chemother. Rept.,51: 125–141, 1967.
13.
TannockI. F.: A comparison of cell proliferation parameters in solid and ascites Ehrlich tumors.Cancer Res.,29: 1527–1534, 1969.
14.
WimberD. E., and QuastlerH. A.: 14C and 3H-thymidine double labeling technique in the study of cell proliferation in tradescantia root tips.Exp. Cell Res.,30: 8–32, 1963.
15.
YoungR. C., De VitaV. T., and PerryS.: The thymidine 14C and 3H double labeling technic in the study of the cell cycle of L 1210 leukemia ascites tumor in vivo.Cancer Res.,29: 1581–1584, 1969.
