Human lymph nodes, normal or with malignant lymphomas, in organ culture present after four days various types of changes (loss of lymphocytes, necrosis, and fibrotic hyaline changes). In many cases these changes do not destroy the original structure. The latter is more recognizable in reactive hyperplasias, in lymphosarcomas, in reticulum cell sarcomas and in chronic lymphocytic leukaemia than in Hodgkin's disease. Severe fibrosis constitutes a fairly characteristic feature differentiating Hodgkin's disease from other lymphomas.
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References
1.
ChenJ. M.: The cultivation in fluid medium of organised liver, pancreas and other tissues of foetal rats. Exp. Cell Res., 7: 518–529, 1954.
2.
GougetP., MandinJ., SerreA., DanaM., et RouxJ.: Culture organotypique de ganglions lymphatiques de lapin après stimulation par un antigène de brucella. Rév. Immun., 32: 231–244, 1968.
3.
HannounC., and BussardA. E.: Antibody production by cells in tissue culture. I. Morphological evolution of lymph node and spleen cells in culture. J. Exp. Med., 123: 1035–1046, 1966.
4.
NilssonN. K.: Histological changes in long term explants of human lymph nodes during lymphoblastoid transformation. Acta Path. Microbiol. Scand. Section A, 79: 243–248, 1971.
5.
PonténJ.: Spontaneous lymphoblastoid transformation of long-term cell cultures from human malignant lymphoma. Int. J. Cancer, 2: 311–325, 1967.
6.
ScherftJ. P.: Normal and pathological lymph node tissue cultured in vitro. I. The normal tissue. Proc. Kon. nederl. Akad. Wet. Ser. C., 68: 38–52, 1965.
7.
ScherftJ. P.: Normal and pathological lymph node tissue cultured in vitro. II. The pathological tissues, and in particular Hodgkin's tissue. Proc. Kon. nederl. Akad. Wet. Ser. C., 70: 280–293, 1967.
8.
SiegelB. V., and SmithM. E.: Tissue culture studies on the lymphomas with particular reference to Hodgkin's disease. Path. Microbiol., 24: 1072–1079, 1961.
9.
SykesJ. A., DmochowskiL., ShullenbergerC. C., and HoweC. D.: Tissue culture studies on human leukemia and malignant lymphoma. Cancer Res., 22: 21–26, 1962.
10.
TrowellO. A.: The culture of lymph nodes in vitro. Exp. Cell Res., 3: 79–107, 1952.
11.
TrowellO. A.: The action of cortisone on lymphocytes in vitro. J. Physiol., London, 119: 274–285, 1953.
12.
TrowellO. A.: The culture of mature organs in a synthetic medium. Exp. Cell Res., 16: 118–147, 1959.
13.
TrowellO. A.: The cytocidal action of mitotic poisons on lymphocytes in vitro. Biochem. Pharmacol, 5: 53–63, 1960.
14.
TrowellO. A.: Cytocidal effects of radiation on organ culture. Ann. N. Y. Acad. Sci, 95: 849–865, 1961.
15.
TrowellO. A.: The optimum concentration of sodium chloride for the survival of lymphocytes in vitro. Exp. Cell Res., 29: 220–224, 1963.
