Mixed lymphocyte cultures in the presence of PHA were performed as follows: (a) normal lymphocytes added to lymphocytes from patients with chronic lymphocytic leukemia; (b) normal lymphocytes from two unrelated subjects. In both cases, lymphocyte transformation in 72 h cultures was often lowered if compared with the values expected according to the sum of the responses to PHA of both partners. Such a decrease is consistent with an interference between the transforming effect of PHA and the stimulation due to the histoincompatibility of the cultured cells. The possibility that in some cultures the two stimulations may result in additional effect is discussed.
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