Showers of cancer cells were detected in the blood of animals bearing an experimental colon carcinoma of the rat following the administration of fluids into the tumor mass. These showers of cancer cells, however, persisted in the circulating blood only for a short period of time.
A significant increase in blood fibrinogen levels and a rapid consumption of factor VIII were also detected after surgery.
Hepatic metastases occurred in 9.2 % of the control animals, in 27.7 % of the sodium pentobarbital treated animals, and in 22.4 % of those inoculated with sodium chloride intratumor.
The significance of the presence of the showers of cancer cells into the circulating blood produced during these procedures, and the possibilities that these may increase metastases is discussed.
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References
1.
AgostinoD., and ClifftonE. E.: Il fattore coagulazione nella produzione di metastasi: Efficacia della fibrinolisina e dell'eparina.Ospedali d'Italia Chirurgia, 9: 393–404, 1964.
2.
AgostinoD.: Sono effettivamente dannose le biopsie dei tumori maligni? (Nota sperimentale).Ospedali d'Italia Chiururgia, 17: 631–637, 1967.
3.
AgostinoD.: Dimostrazione sperimentale della presenza di uno stadio di quiescenza delle cellule neoplastiche.Tumori, 53: 373–378, 1967.
4.
AgosinoD., and ClifftonE. E.: Effect of tissue damage on the fibrinogen levels and the production of pulmonary metastases in rats.Ann. Surg. (in press).
5.
AndreeC.: Beitrag zur Lehre von der Etstehung des Geschwultmetastases auf embolishen wege.Virchow Arch. Pat. Anat., 61: 383–387, 1874.
6.
AshworthT. R.: A case of cancer in which cells similar to those of the tumor were seen in the blood after death.Australian Med. J., 14: 146–147, 1869.
7.
ChanutinA., HorstentineJ. C., ColeW. S., and LudewigS.: Blood plasma in rats following partial hepatectomy and laparotomy.J. Biol. Chem., 121: 247–256, 1938.
8.
ClifftonE. E., and AgostinoD.: Effect of inhibitors of fibrinolytic enzymes on development of pulmonary metastases.I. Nat. Cancer. Inst., 33: 753–763, 1964.
9.
ColeW. H., PakardD., and SouthwickH.: Carcinoma of the colon with special reference to prevention and recurrence.J.A.M.A., 155: 1549–1553, 1954.
10.
ColomboC., MaggiC., and RolfoF.: Presence of neoplastic cells in the peripheal blood of patients with bronchial cancer.Minerva Medica, 49: 725–730, 1958.
11.
DiddleA., ShodesD., HollingsworthJ., and KinlawS.: Cervical carcinoma: Cancer cells in the blood.Am. J. Obst. & Gyn., 78: 582–585, 1959.
12.
EngellH. C.: Cancer cells in the circulating blood.Acta Chirurg. Scandinavica, Suppl. 201, 1955.
13.
EngellH. C.: Cancer cells in the blood: Five to nine years followup study.Ann. Surg., 149: 457–461, 1959.
14.
FisherE. R., and FisherB.: Egperimental evidence in support of the dormant tumor cell.Science, 150: 918–919, 1959.
15.
FletcherW. S., and StewartJ. W.: Tumor cells in the blood with special reference to pre- and post-hepatic blood.Brit. J. Cancer, 13: 33–37, 1959.
16.
GrossiC. E., AgostinoD., and ClifftonE. E.: The effect of human fibrinolysin on pulmonary metastases of the Walker 256 carcinosarcoma.Cancer Res., 20: 605–608, 1960.
17.
HadfieldG.: The dormant cancer cell.Brit. Med. J., 2: 606–610, 1954.
18.
HenriquesS. B., HenriquesO. B., and SelyeH.: Influence of cold in fibrinogen concentration.Proc. Exp. Biol. Med., 71, 82–87, 1949.
19.
HumeR., WestJ. T., MalmgremR. A., and ChuE. A.: Quantitative observations of circulating megakaryocytes in the blood of patients with cancer. New England J.Med., 270: 111–117, 1964.
20.
JohnstonE. H.: Liver embolism to the lung as a complication to trauma. U. S. Naval Forces Med.J, 10: 1143–1147, 1959.
21.
JacksonJ. F.: Hystochemical identification of megakaryocytes from peripheal blood examined for tumor cells.Cancer, 15: 259–262, 1962.
22.
JonassonO., LongL., RobertsS. S., Mc GrewE., and Mc DonaldG. O.: Cancer cells in the circulating blood during operative menagement of genitourinary tumor.J. Urol., 85: 1–12, 1961.
23.
LewisJ. H., FergursonJ. H., FreshJ. W., and ZuckerM. B.: Primary hemorrhagic diseases.J. Lab. Clin. Med., 49: 211–232, 1957.
24.
LangdellR. D., WagnerR. H., and BrinkosueK. M.: Effect of antihemophylic factor on one stage clotting test.J. Lab. Clin. Med., 41: 637–647, 1953.
25.
MarshM. C.: Tumor massage and metastases in mice.Cancer Res., 11: 101–106, 1922.
Mc MillanJ. B.: Embolism of cerebral tissue in the lungs following severe head injury.Am. J. Path., 32: 405–415, 1956.
28.
MelamedM., ClifftonE. E., and SealS. H.: Cancer cells in the peripheal venous blood. A quantitative study of cells of problematic origin.J. Clin. Path., 37: 381–388, 1962.
29.
MinotG. R.: Megakaryocytes in the peripheal circulation.J. Exp. Med., 36: 1–7, 1922.
30.
MooreG. E., SandembergA. A., and SchubargJ. R.: Clinical and experimental observations on the occurrence and fate of tumor cells in the blood stream.Ann. Surg., 146: 580–586, 1957.
31.
MootseG., AgostinoD., and ClifftonE. E.: Alterations of fibrinogen, plasminogen and inhibitors of plasmin with the growth of V2 carcinoma in rabbits.J. Nat. Cancer Inst., 35: 567–572, 1965.
32.
MorleyT. P.: The recovery of tumor cells from venous blood draining cerebral gliomas.Canadian J. Surg., 2: 363–365, 1959.
33.
PatersonL., JamesS., KellyP., and PaeseG.: Isolation of osteogenic sarcoma cells from peripheral blood after biopsy: An experimental report and a clinical note.Proc. Staff. Meet. Mayo Clin., 35: 443–447, 1960.
34.
PeytonW. T.: Danger in the use of local infiltration anesthesia in operation upon malignant tumors.Ann. Surg., 111: 453–458, 1940.
35.
PotterJ. F., and MalgrenR. A.: A new technique for detection of tumor cells in the blood and its applications to the study of the dissemination of cancer.Surg. Forum, 9: 580–583, 1959.
36.
PoolE. H., and DunlopG. R.: Cancer cells in the blood stream.Am. J. Cancer, 21: 99–102, 1934.
37.
PruittJ. C., HilbergH. W., and KaiserR. F.: Malignant cells in the peripheral blood. New England J.Med., 250: 1161–1164, 1958.
38.
QuenselV.: Zur kenntnis des workommens von Geschwulzellen im Zirculierendem blunta.Upsala Lakref Forh, 26: 1–16, 1921.
39.
QuickH. S.: The psysiology and pathology of hemostasis.Lea and FibgerPhiladelphia, 1961.
40.
RapportH., RaumM., and HorrellJ.: Bone marrow embolism.Am. J. Path., 27: 407–433, 1951.
41.
RakerJ. W., TaftP. D., and EdmondsE. E.: Significance of megakaryocytes in serach for tumor cells in the peripheral blood. New England J.Med., 263: 993–996, 1960.
42.
RobertsS. S., HengeshJ. W., Mc GrathR. G., ValaitisJ., Mc GrewE. A., and ColeW. H.: Prognostic significance of cancer cells in the circulating blood.Ann. Surg., 67: 757–762, 1967.
43.
BobertsS. S., JonassonO., LongL. R., Mc GrewE. A., and ColeW. H.: Clinical significance of cancer cells in the circulating blood. Two to five years survival.Ann. Surg., 154: 362–371, 1961.
44.
RobertsS. S., WatneA., Mc GrewE., and ColeW. H.: Technique and results of isolation of cancer cells from the circulating blood.AMA Arch. Surg., 76: 334–346, 1958.
45.
SealS. H.: Silicone flotation: A simple quantitative method for the isolation of free flotating cancer cells in the blood.Cancer, 12: 500–505, 1959.
46.
ShleipK.: Zur diagnose von knochenmarkstumorem aum dem blutefunde.Ztsch. Kil. Med., 59: 261–282, 1806.
47.
SickP.: Zur Entwckelungschichte von krebs.Vircows Arch. Pat. Anat., 31: 265–269, 1864.
48.
SpriggsA. J.: Discussion on exfoliative cytology in diagnosis of malignant diseases.Proc. Roy. Soc. Med., 53: 169–171, 1960.
TaylorF., and VelliosF.: Failure to prove identity of tumor cells in the peripheal blood.Surgery, 44: 453–456, 1958.
51.
ThierschK.: The ephithelialkrebs namentlich der Haut.Leipzing, 1865
52.
TraykovichV., and RiviereM. B.: Megakaryocytes in the blood extramedullary hematopoiesis.Cancer, 19: 1717–1724, 1966.
53.
TyzzerE. E.: Factors in the production and growth of tumor metastases.J. Med. Res., 28: 309–332, 1913.
54.
WallmannH.: Beitrage zur Lehre der emboli.Vircow Arch. Pat. Anat., 13: 550–554, 1858.
55.
WardG. B.: The blood in cancer with bone metastases.Lancet, 1: 676–677, 1913.
56.
WatneA. L., SandembergA. A., and MooreG. E.: Prognostic application of tumor cells in the blood.Proc. Am. Ass. Cancer Res., 3: 160–165, 1960.
57.
WatneA., RobertsS. S., Mc GrewE., Mc GrathR., and ColeW. H.: The occurrence of cancer cells in the circulating blood and their response to chemotherapy and surgical stress.Acta Unio Contra Cancrum, 16: 790–799, 1960.
58.
WillisR. A.: The spread of tumors in the human body.2nd Ed.London Butterworth, p. 15–19, 1952.
59.
WoodS.Jr.: Experimental studies of the intravascular dissemination of ascite V2 carcinoma cells in the rabbit, with special reference to fibrinogen and fibrinolytic agents.Swiss Acad. Med. Sci., 20: 92–121, 1964.
60.
YoungJ. S., LumsdenC. B., and StalkerA. L.: The significance of « tissue damage » on normal testicular and neoplastic (Brown Pierce carcinoma) tissue in rabbits.J. Path. Bact., 62: 113–114, 1950.
61.
YoungJ. S., and GriffithH. D.: The dynamics of parenchimatous embolism in relation to the dissemination of malignant tumors.J. Path. Bact., 62: 293–311, 1950.
