Effect of Hematoporphyrin « in Vivo » and « in Vitro » on ATPC+ Tumor Cells

Abstract

ATPC+ ascites tumor transplants of different size were performed in NMRI mice. Hematoporphyrin chlorhydrate (Hp) was administered i.p. once daily during the seven days following transplantation. In the first series « in vivo » (1.9 x 10⁶ cells; 0.1, 0.3, 0.9 mg of Hp), body weight, ascitogenic time (T_asc) and total ascitic volume (TV) were determined. Hematoporphyrin-treated animals showed, after the apprearance of the ascites, a body weight loss superior to controls, due to the drug toxicity caused by Hp vs. ascitic fluid interaction. Neither T_asc nor TV were significantly modified. In the second series « in vivo » (2.3 x 10⁴ or 2.3 x 10² cells; 0.3 mg Hp), body weight loss was lower, T_asc was significantly increased and also the survival varied. In a third experiment ATPC+ cells were incubated « in vitro » with Hp in a range from 10² to 10⁸ molecules/cell). No effect was observed on growth rate when the cells were transplanted into the host.