Subcutaneous Carcinogenesis by Implants and by 7,12-Dimethylbenz[a]Anthracene 1

Abstract

Italian

The effects of the combined administration of urethan or Trypan Blue to the s.c. injection of 7,12-dymethylbenz[a]anthracene (DMBA) were investigated.

The repeated administration of Trypan Blue resulted in a marked inhibition of sarcomas arising around Teflon disc implanted s.c. It was thus confirmed, in a different strain of mice, the result of a previous experiment, where Trypan Blue was shown to exert an inhibiting action on the sarcoma production by glass fragments implanted subcutaneously. No consistent inhibiting action of Trypan Blue on the sarcoma production by DMBA could be demonstrated.

Urethan administered before, concurrently or at various time after DMBA administration had no apparent effect on the incidence of sarcomas induced by DMBA. In a previous experiment urethan was showed to enhance the incidence of sarcomas arising around Teflon films.

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References

Andervont

H. B.

: The influence of Trypan Blue upon the resistance of mice to transplantable and induced tumors. Public Health Rep., 51: 591–600, 1936.

Bates

R. R.

and Prehn

R. T.

: Role of the fibrous capsule in carcinogenesis by plastic film. Nature, 205: 303–304, 1965.

Benacerraf

: The effct of enhancement of natural resistance upon tumor rejection. Acta Unio Intern. contra Cancrum, 19: 73–76, 1963.

Berenblum

and Trainin

: Possible two-stage mechanism in experimental leukemogenesis in mice. Cancer Res., 18: 725–729, 1958.

Berenblum

Rewald

F. E.

and Trainin

: Failure of bone marrow to interfere with the augmentation of X-ray leukemogenesis by urethan. J. Nat. Cancer Inst., 27: 1361–1367, 1961.

Berenblum

and Trainin

: The lack of effect of urethan (ethyl carbamate) on sarcoma induction by the subcutaneous injection of 3,4-benzpyrene. Cancer Res., 23: 983–986, 1963.

Bischoff

and Bryson

: Carcinogenesis through solid state surfaces. Progr. Exp. Tumor Res., 5: 85–133, 1964.

Brown

D. V.

: Subcutaneous neoplasms induced by Trypan Blue. Acta Unio Intern. contra Cancrum, 19: 655–656, 1963.

Brown

D. V.

Boehni

E. M.

and Norlind

L. M.

: Anemia with positive direct Coombs test induced by Trypan Blue. Blood, 18: 543–560, 1961.

10.

Brown

D. V.

Norlind

L. M.

Adamovics

and Bowen

: Studies on serum proteins changes and organ dye concentrations in Thypan Blue carcinogenesis. Proc. Soc. Exp. Biol. Med., 114: 290–293, 1963.

11.

Brown

D. V.

and Thorson

T. A.

: Reticulum-cell sarcoma of rats. Experimental production with Trypan Blue. J. Nat. Cancer Inst., 16: 1181–1195, 1956.

12.

Chieco-Bianchi

Fiore-Donati

De Benedictis

and Tridente

: Influence of urethane on susceptibility to leukaemia induction by Graffi virus in adult mice. Nature, 199: 292–293, 1963.

13.

Di Luzio

N. R.

Simon

K. A.

Upton

A. C.

: Effects of X-rays and Trypan Blue on reticuloendothelial cells. A.M.A. Arch. Path., 64: 648–656, 1957.

14.

Foulds

: The effect of vital staining on the distribution of the Brown-Pearce rabbit tumor. 10th Sc. Rep. Imp. Cancer Res. Fund, 21–31, 1932.

15.

Gillman

Th.

and Gilbert

: The pathogenesis of experimental produced lymphomata in rats (including Hodgkin's like sarcomas). Cancer, 5: 792–846, 1952.

16.

Hueper

W.C.

: Cancer induction by polyurethan and polysilicone plastics. J. Nat. Cancer Inst., 33: 1005–1027, 1964.

17.

Kawamoto

Ida

Kirschbaum

and Taylor

H. G.

: Urethan and leukemogenesis in mice. Cancer Res., 18: 725–729, 1958.

18.

Kirschke

und Graffi

: Versuche zur Abhängigkeit der Wirkung des Virus der myeloischen Leukämie der Maus von verschiedenen biologischen Bedingungen der infizierten Tiere. Arch. Geschwulstforsch., 20: 22–29, 1962.

19.

Klein

Sjogren

H. O.

and Klein

: Demonstration of host resistance against sarcomas induced by implantation of cellophane films in isologous (syngeneic) recipients. Cancer Res., 23: 84–92, 1963.

20.

Law

L. W.

and Precerutti

: Inhibition by urethan (ethyl carbamate) of virus induction of leukaemia in C3H mice. Nature, 200: 692–693, 1963.

21.

Ludford

R. J.

: Resistance to the growth of transplantable tumors. I. The influence of vital staining on induced resistance. Brit. J. Exper. Path., 12: 45–49, 1931.

22.

Ludford

R. J.

: Resistance to the growth of transplantable tumors. II. The influence of dyestuff and colloids in natural resistance. Brit. J. Exper. Path., 12: 108–113, 1931.

23.

Mahaley

M. S.

Jr. , and Knisely

H. W.

: Effect of Trypan Blue upon distribution of metastasis and transplants of rabbits VX2 and Walker rat 56 tumors. Cancer Res., 19: 627–632, 1959.

24.

Marshall

A. H. E.

: The production of tumors of the reticular tissue by azodyes. Acta Pat. Microb. Scand., 33: 1–9, 1953.

25.

Old

L. J.

Benacerraf

Clarke

D. A.

Carswell

E. A.

and Stockert

: The role of the reticuloendothelial system in the host reaction to neoplasia. Cancer Res., 21: 1281–1300, 1961.

26.

Old

L. J.

Boyse

A. E.

Clarke

D. A.

and Carswell

E. A.

: Antigenic properties of chemically induced tumors. Ann. N. Y. Acad. Sci., 101: 80–106, 1962.

27.

Ooneda

Matsuyama

Oka

Ninomiya

Araki

and Takano

: Experimental studies on reticulum cell sarcoma and reticulosis. Gunna J. Med. Sci., 6: 296–317, 1957.

28.

Oppenheimer

B. S.

Oppenheimer

E. T.

Stout

A. P.

Willhite

and Danishefsky

: The latent period in carcinogenesis by plastics in rats and its relation to the presarcomatous stage. Cancer, 11: 204–213, 1958.

29.

Oppenheimer

E. T.

Willhite

Stout

A. P.

Danishefsky

and Fishman

M. M.

: A comparative study on the effects of imbedding cellophane and polystyrene films in rats. Cancer Res., 24: 379–387, 1964.

30.

Rawson

R. A.

: The bindging of T-1824 and structurally related diazo dyes by the plasma ploteins. Amer. J. Physiol., 138: 708–717, 1934.

31.

Ruttner

J. R.

und Brunner

H. E.

: Zur Frage einer carcinogenen Wirkung der Azofarbstoffe Evans Blue und Trypanblau auf das Retikuloendotheliale System del Leber. Schweiz. Z. Path. Bakt., 19: 436–455, 1956.

32.

Ruttner

J. R.

und Brunner

H. E.

: Zur Frage der experimentellen Induktion von Tumoren des lymphatischen Systems der Ratte mit den Diazo-Farbstoffen Trypanblau und Evans Blue. Schweiz. Z. Path. Bakt., 22: 219–535, 1959.

33.

Saphir

and Appel

: Attempts to abrogate immunity to the Brown Pearce carcinoma. Cancer Res., 3: 767–769, 1943.

34.

Schmahl

Thomas

und Brune

: Experimentelle Untersuchungen zur Syncarcinogenese. II. Versuche zur Krebserzeugung dei Mäusen bei gleichzeitiger Applikation von Urethan und 9,10-Dymethyl-1,2-benzanthracen. Z. Krebsforsch., 66: 297–302, 1964.

35.

Shubik

Della Porta

Spencer

and Pietra

: The inhibition of 9,10-dimethyl-1,2-benzanthracene skin carcinogenesis by simultaneous application of urethan. Proc. Am. Ass. Cancer Res., 2: 345–346, 1958.

36.

Shubik

: Carcinogenesis: The present position. Can. Cancer Conf., 6: 244–253, 1966.

37.

Simpson

: Trypan Blue induced tumors on rats. Brit. J. Exp. Path., 33: 524–528, 1952.

38.

Stern

: Effect of sulfonated azo dyes on mouse tumors. Cancer Res., 10: 565–570, 1950.

39.

Tomatis

: Studies in subcutaneous carcinogenesis with implants of glass and Teflon in mice. Acta Unio Intern. contra Cancrum, 19: 607–611, 1963.

40.

Tomatis

and Shubik

: Influence of urethane on subcutaneous carcinogenesis by Teflon implants. Nature, 198: 600–601, 1963.

41.

Vasiliev

J. M.

Olshevskaya , Raikhlin

N. T.

and Ivanova

O. J.

: Comparative study of alterations induced by 7,12-Dimethylbenz(a)anthracene and polymer films in the subcutaneous connective tissue of rats. J. Nat. Cancer Inst., 28: 515–559, 1962.

42.

Yamada

: Abnormal serum protein observated in Trypan Blue treated rats. Proc. Soc. Exp. Biol. Med., 101: 566–568, 1959.