Thymuses of newborn and adult mice were maintained as organ cultures for 20 days. A 24 hour treatment with urethan or with 7,12-dimethylbenz(a)anthracene (DMBA) induced necrosis of the peripheral zone of the cortex in both the newborn and adult cultured thymuses. In all the DMBA treated thymuses and the newborn thymuses treated with urethan, the necrosis eventually involved a large part of the organs. In the adult thymuses treated with urethan the necrosis appeared notably reduced at the end of the 20 day observation period.
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