Restricted accessResearch articleFirst published online 1962-11
Influence of a Derivative of Testosterone on the Growth of the Spontaneous Mammary Adenocarcinoma of the Mouse « in vitro »: 4-hydroxy-17alpha-methyl-19-nortestosterone
Testosterone propionate and 4-hydroxy-17 alfa-methyl-19-nortestosterone are being tested against a mouse mammary tumor growing in vitro, treated with 10 μg/cc for 8-9-12 days. The general procedure used in these tests have been indicated. Testosterone inhibits the tumor growth without influencing its cytological differentiation. The inhibiting action of testosterone is accompanied by regressive phenomena of the tumor elements. 4-hydroxy-17 alfa-methyl-19-nor-testosterone inhibits strongly the tumor growth with a decrease in mitosis number. It is possible sometimes to observe mitosis with lagging chromosomes. The histopathological structures are not influenced by hormonal treatment.
Get full access to this article
View all access options for this article.
References
1.
BardiU., BorettiG., and Di MarcoA.: Influence of steroids on the metabolism of rat liver mitochondria and of hepatoma ascites cells in vitro.Biochem. Pharm., 11: 1055–1065, 1962.
2.
BimesC., PlanelH., et DavidJ. F.: Action des hormones génitales sur la souche tumorale HeLa et sur diverses categories cellulaires normales cultivés in vitro.C.R. Soc. Biol., 155: 138–141, 1961.
3.
Di MarcoA., GaetaniM., DorigottiL., e BelliniO.: Attività del 4-idrossi-17 alfa-metil-19-nortestosterone su alcuni tumori sperimentali.Tumori, 48: 361–372, 1962.
4.
EndoH., WakabayashiK., EnomotoH., KatsutaK., and TakaokaT.: Studies of the action of hormones in tissue culture. (III) Leucine aminopeptidase activity of the strain HeLa cells (cervical carcinoma of human uterus) and the effects of sex hormones.Gann, 51: suppl., 236–237, 1960.
5.
FlandreO., Crastes de PauletA., et FlochH.: Action de quelques phosphatases de steroide sur des fibroblastes en culture in vitro.C.R. Soc. Biol., 155: 2408–2410, 1962.
6.
HeusonJ. C., and LegrosN.: In vitro effect of testosterone and 17 beta-estra-diol on protein metabolism.Cancer Chem. Rep., 16: 277–278, 1962.
7.
KawadaJ.: Effects of progesterone and estrogens on the multiplication of chick-embryo fibroblasts, rat ascite hepatoma cells, and strain HeLa cells (cervical carcinoma of human uterus) in tissue culture.Japan J. Exp. Med., 29: 615–619, 1959.
8.
LettréH.: Cytotoxic hormones, in Biological Approaches to Cancer Chemotherapy.HarrisR. J. C. (Ed.), Academic Press Inc., London, 1961, p. 285–293.
9.
MonesiV.: Influenza degli ormoni sessuali sulla crescita dell'adenocarcinoma mammario spontaneo del topo in vitro.Tumori, 42: 334–360, 1956.
10.
OmuroS., KikuchiF., and IchinoeK.: On the ihibiting effect of steroid hormones on HeLa cell proliferation.Gann, 50: Suppl., 180–181, 1959.
11.
RotaM.: Azione di un nuovo ormone maschile sull'adenocarcinoma mammario murino coltivato in vitro.Tumori, 44: 358–366, 1958.
