Abstract
The dialysable fraction of ascitic fluids, that depresses liver catalase activity, has been studied. This fraction was extracted from ascitic fluids (from cancer patients with peritoneal spread, from cirrhotic patients and from mice bearing' sarcoma 180), after various treatments, by n-butanol at pH 10. It proved to be adsorbed to globulins and to confer them their anticatalasic activity.
A chromatographic analysis of the very active material demonstrated its complexity and the plurality of the inhibiting substances. The extreme lability of these factors has, until now, prevented their isolation in a pure state. When degraded, their activity is partially restored by reducing agents. On the basis of several data, it is supposed that they are basic polypeptides with SH and phenolic OH groups, and that they are bound, in the ascitic fluid, to a protein support, probably of glubulinic type.
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