Adoptive immunotherapy can be a therapeutic option to treat cancer patients with advanced-stage disease refractory to conventional therapies.
Methods and study design
We used T-cell therapy with autologous antitumor cytotoxic T lymphocytes (CTLs) in a patient with metastatic renal cell carcinoma. Autologous antitumor CTLs obtained by stimulating CD8-enriched cells with dendritic cells pulsed with irradiated apoptotic tumor cells and expanded in vitro were transfused on days +14 and +28 following chemotherapy and every 2 to 4 months thereafter.
Results and Conclusions
Treatment with high doses of antitumor CTLs proved to be safe and induced immunological responses and long-lasting clinical benefit in our patient.
PortaC., PaglinoC., ImarisioI., GaniniC., PedrazzoliP.: Immunological effects of multikinase inhibitors for kidney cancer: a clue for integration with cellular therapies?J Cancer, 2: 333–338, 2011.
3.
TurinI., PedrazzoliP., TullioC., MontiniE., La GrotteriaM.C., SchiavoR., PerottiC., LocatelliF., CarrettoE., MaccarioR., SienaS., MontagnaD.: GMP production of anti-tumor cytotoxic T-cell lines for adoptive T-cell therapy in patients with solid neoplasia. Cytotherapy, 9: 499–507, 2007.
4.
MontagnaD., TurinI., SchiavoR., MontiniE., ZaffaroniN., VillaR., SecondinoS., SchiavettoI., CaliognaL., LocatelliF., LibriV., PessionA., TonelliR., MaccarioR., SienaS., PedrazzoliP.: Feasibility and safety of adoptive immunotherapy with ex vivo-generated autologous, cytotoxic T lymphocytes in patients with solid tumor. Cytotherapy, 14: 80–90, 2012.
5.
MotzerR.J., MazumandarM., BacikJ., BergW., AmsterdamA., FerraraJ.: Survival and prognostic stratification of 670 patients with advanced renal cell carcinoma. J Clin Oncol, 17: 2530–2540, 1999.
6.
RosenbergS.A., DudleyM.E.: Adoptive cell therapy for treatment of patients with metastatic melanoma. Curr Opin Immunol, 21: 233–240, 2009.
7.
MontagnaD., SchiavoR., GibelliN., PedrazzoliP., TonelliR., PaganiS., AssirelliE., LocatelliF., PessionA., FregoniV., MontiniE., Da PradaG.A., SienaS., MaccarioR.: Ex vivo generation of anti-tumor cytotoxic T-cell lines derived from patients and their HLA-identical sibling. Int J Cancer, 110: 76–86, 2004.
