T1 and T2 were measured in 5 formalin-fixed human whole-brain specimens as a function of time. Gray matter/white matter contrast reversal was observed around the 4th day and was considered to be due to the greater decrease in T1 in gray than in white matter. A possible explanation for this is that the decomposition of the myelin phospholipid structure by formalin somewhat counteracts the general reductive effect of the fixation procedure on relaxation times.
AwadI.JohnsonP.SpetzlerR.HodakJ.: Incidental subcortical lesions identified on MRI in the elderly. II. Postmortem pathological correlationsStroke176 (1986), 1090.
2.
BraffmanB. H.ZimmermanR. A.TrojanowskiJ. Q.GonatasN. K.HickeyW. F.SchlaepferW. W.: Brain MR. Pathologic correlation with gross and histopathology. 1. Lacunar infarction and Virchow-Robin spaces. AJR151 (1988), 551.
3.
BranteG.: Studies on lipids in the nervous system with special reference to quantitative chemical determination and topical distribution. Acta Physiol. Scand. 18, suppl. 63 (1949), 180.
4.
FoxC.JohnsonF.WhitingJ.RollerP.: Formaldehyde fixation. J. Histochem. Cytochem.338 (1985), 845.
5.
GrafeM.PressG.BerthotyD.HesselinkJ.ClaytonW.: Abnormalities of the brain in AIDS patients. Correlation of postmortem MR findings with neuropathology. AJNR115 (1990), 905.
6.
HirschW.JungreisC.MartinezA.MoossyJ.LatchawR.WolfeG.: Postmortem MR imaging of the brain. (Abstract)AJNR8 (1987), 964.
7.
KammanR. L.GoK. G.StompG. P.HulstaertC. E.BerendsenH. J. C.: Changes of relaxation times T1 and T2 in rat tissues after biopsy and fixation. Magn. Reson. Imaging3 (1985), 245.
8.
KoenigS.BrownR.IIISpillerM.LundbomN.: Relaxometry of brain. Why white matter appears bright in MRI. Magn. Reson. Med.14 (1990), 482.
9.
KoenigS.: Cholesterol of myelin is the determinant of gray-white matter contrast in MRI of brain. Magn. Reson. Med.20 (1991), 285.
10.
NagaraH.InoueT.KogaT.KitaguchiT.TateishiJ.GotoI.: Formalin fixed brains are useful for MRI study. J. Neurol. Sci.81 (1987), 67.
11.
NixonJ.MillerG.OkazakiH.GomezM.: Cerebral tuberous sclerosis. Postmortem MRI and pathologic anatomy. Mayo Clin. Proc.64 (1989), 305.
12.
SperberG. O.EricssonA.HemmingssonA.: A fast method for T1 fitting. Magn. Reson. Med.9 (1989), 113.
13.
ThickmanD.KundelH.WolfG.: NMR characteristics of fresh and fixed tissue. The effect of elapsed time. Radiology148 (1983), 183.
14.
ThompsonS. W.: Selected histochemical and histopathological methods. Charles C. Thomas, Springfield, IL1966.
15.
TorackR.AlcalaH.GadoM.BurtonR.: Correlative assay of computerized cranial tomography (CCT), water content and specific gravity in normal and pathological postmortem brain. J. Neuropathol. Exp. Neurol.35 (1976), 385.
16.
UngerE.GadoM.FullingK.LittlefieldJ.: Acute cerebral infarction in monkeys. An experimental study using MRI. Radiology.162 (1987), 789.
