The under agarose method for evaluation of leucocyte chemotaxis was used to investigate the effect of radiographic contrast media (CM) on granulocyte locomotion. The CM tested had no chemoattractive properties. CM inhibited N-fmlp, a synthetic formylated Met-tripeptide, which is a strong chemotactic agent and an analogue to chemotatic peptides produced by bacteria. The inhibition of N-fmlp was most pronounced for diatrizoate. Equiosmolal saline was not so inhibitive. Therefore, some part of the inhibition was caused by factor(s) other than hyperosmolality inherent in the CM solution.
. BøyumA.: Isolation of mononuclear cells and granulocytes from human blood. Scand. J. Clin. Lab. Invest.21 (1968) Suppl. No. 97, p. 77.
2.
. CarrD. H.WalkerA. C.WhiteR. G.: Effects of radiographic contrast media on leucocyte locomotion. Invest. Radiol.16 (1981), 133.
3.
. ChenowethD.E.RoweJ. G.HugliT. E.: A modified method for chemotaxis under agarose. J. Immunol. Meth.25 (1979), 337.
4.
. FerranteA.ThongY. H.: A rapid one-step procedure for purification of mononuclear and polymorphonuclear leucocytes from human blood using a modification of the Hypaque/Ficoll technique. J. Immunol. Meth.24 (1978), 389.
5.
. GeorgsenJ.RasmussenF.PedersenJ. O.: The effect of radiographic contrast media on the chemotaxis of leucocytes. Invest. Radiol.23 (1988), 621.
6.
. LasserE. C.LangJ. H.LyonS. G.HamblinA. E.: Changes in complement and coagulation factors in a patient suffering a severe anaphylactoid reaction to injected contrast material. Some considerations of pathogenesis. Invest. Radiol.15 (1980), S6.
7.
. NelsonR. D.QuieP. G.SimmonsR. L.: Chemotaxis under agarose. A new and simple method for measuring chemotaxis and spontaneous migration of human polymorphonuclear leucocytes and monocytes. J. Immunol.115 (1975), 1650.
8.
. PedersenJ. O.: Gold-medal thesis in clinical chemistry, Odense University1985. (In Danish.) Available at Odense University Library.
9.
. RasmussenF.GeorgsenJ.GrunnetN.: Influence of radiographic contrast media on phagocytosis. Acta. Radiol.29 (1988), 589.
10.
. ShehadiW. H.: Contrast media adverse reactions. Occurrence, recurrence, and distribution patterns. Radiology143 (1982), 11.
11.
. SlivkaJ.LasserE. C.LangJ. H.LyonS. G.HamblinA. E.: Effect of endotoxemia on contrast media reactions. Invest. Radiol.13 (1978), 21.
12.
. TillG.RotherU.GemsaD.: Activation of complement by radiographic contrast media. Generation of chemotactic and anaphylatoxin activities. Int. Arch. Allergy Appl. Immunol.56 (1978), 543.
