Two independent systems were developed to produce antibodies to diatrizoate. In the rabbit model polyclonal antibodies were produced that showed cross-reactivity to certain other contrast agents, analogues and serum proteins. In the mouse model monoclonal antibodies were produced that reacted only with diatrizoate, metrizamide, and ovalbumin. These studies demonstrated the potential antigenicity of contrast medium.
AvrameasS.: Coupling of enzymes to proteins with gluteraldehyde. Immunochemistry6 (1969), 43.
3.
BraschR. C.CaldwellJ. L.: The allergic theory of radiocontrast agent toxicity. Demonstration of antibody activity in sera of patients suffering major radiocontrast agent reactions. Invest. Radiol.11 (1976), 347.
4.
CarrD. H.WalkerA. C.: Contrast media reactions. Experimental evidence against the allergy theory. Brit. J. Radiol.57 (1984), 469.
5.
ChenaisF.VirellaG.PatrickC. C.: Isolation of soluble immune complexes by affinity chromatography using staphylococcal protein A-sepharose as substrate. J. Immunol. Meth.18 (1977), 183.
6.
DunnC. R.LasserE. C.SellS.LangJ.: Failure to induce hypersensitivity reactions to opaque contrast media analogs in guinea pigs. Invest. Radiol.10 (1975), 317.
7.
EdwardsD. P.GrzybK. T.DresslerL. G. et coll.: Monoclonal antibody identification and characterization of a MT 43000 membrane glycoprotein associated with human breast cancer. Cancer Res.46 (1986), 1306.
8.
KleinknechtD.DelouxJ.HombergJ. C.: Acute renal failure after intravenous urography. Detection of antibodies against contrast media. Clin. Nephr.2 (1974), 116.
9.
LambinP.FineJ. M.: Molecular weight estimation of proteins by electrophoresis in linear polyacrylamide gradient gels in the absence of denaturing agents. Ann. Biochem.98 (1979), 260.
10.
NilssonR.EhrenbergL.FedorcsakI.: Formation of potential antigens from radiographic contrast media. Acta Radiol.28 (1987), 473.
11.
SchonheyderH.AndersonP.: Effects of bovine serum albumin on antibody determination by the enzyme-linked immunosorbant assay. J. Immunol. Meth.72 (1984), 251.
12.
SweeneyJ. J.KlotzS. O.: Frequency of IgE-mediated radio contrast dye reactions. J. All. Clin. Immunol.71 (1983), 147.
13.
Wakkers-GarritsenB. G.HouwerzijiJ.NaterJ. P.WakkersP. J. M.: IgE-mediated adverse reactivity to a radiographic contrast medium. Ann. All.36 (1976), 122.
14.
WalkerA. C.CarrD. H.: Reactions to radiographic contrast media. An attempt to detect specific anti-contrast medium antibodies in the sera of reactor patients. Brit. J. Radiol.59 (1986), 531.