Transport of individual cells or chemical payloads on a subcellular scale is an enabling tool for the study of cellular communication, cell migration, and other localized phenomena. We present a magnetically actuated robotic system capable of fully automated manipulation of cells and microbeads. Our strategy uses autofluorescent robotic transporters and fluorescently labeled microbeads to aid tracking and control in optically obstructed environments. We demonstrate automated delivery of microbeads infused with chemicals to specified positions on neurons. This system is compatible with standard upright and inverted light microscopes and is capable of applying forces less than 1 pN for precision positioning tasks.
AbbottJJNagyZBeyelerFNelsonBJ (2007) Robotics in the small, part I: Microrobotics. IEEE Robotics and Automation Magazine14: 92–103.
2.
BarneaDISilvermanHF (1972) A class of algorithms for fast digital image registration. IEEE Transactions on ComputersC-21: 179–186. DOI: 10.1109/TC.1972.5008923.
3.
BarrettLESulJYTakanoHBockstaeleEJVHaydonPG (2006) Region-directed phototransfection reveals the functional significance of a dentrically synthesized transcription factor. Nature Methods3: 455–460.
4.
ChiouPYOhtaATWuMC (2005) Massively parallel manipulation of single cells and microparticles using optical imaging. Nature436: 370–372.
5.
DameanNParvizBALeeJNOdomTWhitesidesGM (2005) Composite ferromagnetic photoresist for the fabrication of microelectromechanical systems. Journal of Micromechanics and Microengineering15: 29–34.
6.
DaugeMGauthierMPiatE (2007) Modelling of a planar magnetic micropusher for biological cell manipulations. Sensors and Actuators A138: 239–247.
7.
DesaiJPPillarisettiABrooksAD (2007) Engineering approaches to biomanipulation. Annual Review of Biomedical Engineering9: 35–53.
8.
FernandesRGraciasDH (2009) Toward a miniaturized mechanical surgeon. Materials Today12: 14–20.
9.
FischerPGhoshA (2011) Magnetically actuated propulsion at low Reynolds number: towards nanoscale control. Nanoscale3: 557–563.
10.
FloydSPawasheCSittiM (2009) Two-dimensional contact and noncontact micromanipulation in liquid using an untethered mobile magnetic microrobot. IEEE Transactions on Robotics25: 1332–1342.
11.
FreibergSZhuXX (2004) Polymer microspheres for controlled drug release. International Journal of Pharmaceutics282: 1–18.
12.
FrutigerDRVollmersKKratochvilBENelsonBJ (2010) Small, fast, and under control: wireless resonant magnetic micro-agents. The International Journal of Robotics Research29: 613–636.
13.
GauthierMPiatE (2004) An electromagnetic micromanipulation systems for single-cell manipulation. Journal of Micromechatronics2: 87–119.
14.
GhoshAFischerP (2009) Controlled propulsion of artificial magnetic nanostructured propellers. Nano Letters9: 2243–2245.
15.
HagiwaraMKawaharaTYamanishiYMasudaTFengLAraiF (2011) On-chip magnetically actuated robot with ultrasonic vibration for single cell manipulations. Lab on a Chip11: 2049–2054.
16.
HuWIshiiKSFanQOhtaAT (2012) Hydrogel microrobots actuated by optically generated vapour bubbles. Lab on a Chip12: 3821–3826.
17.
JagerEWHInganasOLundstromI (2000) Microrobots for micrometer-size objects in aqueous media: potential tools for single-cell manipulation. Science288: 2335–2338.
KummerMPAbbottJJKratochvilBEBorerRSengulANelsonBJ (2010) Octomag: An electromagnetic system for 5-dof wireless micromanipulation. IEEE Transactions on Robotics26: 1606–1617.
20.
LeongTGRandallCLBensonBRBassikNSternGMGraciasDH (2009) Thetherless thermobiochemically actuated microgrippers. Proceedings of the National Academy of Sciences106: 703–708.
21.
LinderVGatesBDRyanDParvizBAWhitesidesGM (2005) Water-soluble sacrificial layers for surface micromachining. Small7: 730–736.
22.
NelsonBJKaliakatsosIKAbbottJJ (2010) Microrobots for minimally invasive medicine. Annual Review of Biomedical Engineering12: 55–85.
23.
NeumanKCNagyA (2008) Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy. Nature Methods5: 491–505.
24.
PawasheCFloydSSittiM (2009) Modeling and experimental characterization of an untethered magnetic micro-robot. The International Journal of Robotics Research28: 1077–1094.
25.
RidleyAJSchwartzMABurridgeK. (2003) Cell migration: Integrating signals from front to back. Science302: 1704–1709.
26.
SakarMSSteagerEBCowleyAKumarVPappasGJ (2011) Wireless manipulation of single cells using magentic microtransporters. In: IEEE International Conference on Robotics and Automation, pp. 2668–2673.
27.
SakarMSSteagerEBKimDH. (2010a) Biosensing and actuation for microbiorobots. In: IEEE International Conference on Robotics and Automation. Anchorage, AL, pp. 3141–3146.
28.
SakarMSSteagerEBKimDHKimMJPappasGJKumarV (2010b) Single cell manipulation using ferromagnetic composite microtransporters. Applied Physics Letters96: 043705.
TottoriSZhangLQiuFKrawczykKKFranco-ObregonANelsonBJ (2012) Magnetic helical micromachines: fabrication, controlled swimming, and cargo transport. Advanced Materials24: 811–816.
31.
VoldmanJ (2006) Electrical forces for microscale cell manipulation. Annual Review of Biomedical Engineering8: 425–454.
32.
YesinKBVollmersKNelsonBJ (2006) Modeling and control of untethered biomicrorobots in a fluidic environment using electromagnetic fields. The International Journal of Robotics Research25: 527–536.
33.
ZhangLPeyerKENelsonBJ (2010) Artificial bacterial flagella for micromanipulation. Lab on a Chip10: 2203–2215.
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