Early adolescence is a critical period characterized by challenges and increased peer interactions. The literature presents conflicting findings on the relationship between stress and prosocial behaviors, with no data on perceived ongoing stress and cooperation. Additionally, the protective role of Cardiac Vagal Tone (CVT), a biological marker of self-regulation, has not been studied in this context. This study aims to fill this gap by examining 117 early adolescents. Participants completed questionnaires on demographics and perceived stress. ECG was recorded to compute CVT, and cooperative behavior was assessed using the Slingshot Challenge Game. Results indicated that perceived stress negatively correlated with cooperation, while CVT showed a U-shaped relationship with cooperation. CVT moderated the perceived stress-cooperation link, suggesting that higher stress decreased cooperation only in adolescents with lower CVT. These findings highlight CVT’s potential as a protective factor, indicating that interventions to enhance CVT may improve cooperative behaviors in stressed early adolescents.
AhmedS. P.Bittencourt-HewittA.SebastianC. L. (2015). Neurocognitive bases of emotion regulation development in adolescence. Developmental Cognitive Neuroscience, 15(1), 11–25. https://doi.org/10.1016/j.dcn.2015.07.006
2.
AlenN. V.DeerL. K.KarimiM.FeyzievaE.HastingsP. D.HostinarC. E. (2021). Children’s altruism following acute stress: The role of autonomic nervous system activity and social support. Developmental Science, 24(5), Article e13099. https://doi.org/10.1111/desc.13099
3.
AlsayedA.WildesV. J. (2018). Syrian refugee children: A study of strengths and difficulties. Journal of Human Rights and Social Work, 3(2), 81–88. https://doi.org/10.1007/s41134-018-0057-4
4.
BauerD. J.CurranP. J. (2005). Probing interactions in fixed and multilevel regression: Inferential and graphical techniques. Multivariate Behavioral Research, 40(3), 373–400. https://doi.org/10.1207/s15327906mbr4003_5
5.
BauerM.BlattmanC.ChytilováJ.HenrichJ.MiguelE.MittsT. (2016). Can war foster cooperation?The Journal of Economic Perspectives, 30(3), 249–274. https://doi.org/10.1257/jep.30.3.249
6.
BeauchaineT. (2001). Vagal tone, development, and Gray’s motivational theory: Toward an integrated model of autonomic nervous system functioning in psychopathology. Development and Psychopathology, 13(2), 183–214. https://doi.org/10.1017/S0954579401002012
7.
BeauchaineT. P. (2015). Respiratory sinus arrhythmia: A transdiagnostic biomarker of emotion dysregulation and psychopathology. Current Opinion in Psychology, 3, 43–47. https://doi.org/10.1016/j.copsyc.2015.01.017
8.
BerntsonG. G.Thomas BiggerJ. , J.EckbergD. L.GrossmanP.KaufmannP. G.MalikM.PorgesS. W.SaulJ. P.StoneP. H.Van Der MolenM. W. (1997). Heart rate variability: Origins, methods, and interpretive caveats. Psychophysiology, 34(6), 623–648. https://doi.org/10.1111/j.1469-8986.1997.tb02140.x
9.
BodenmannG.MeuwlyN.GermannJ.NussbeckF. W.HeinrichsM.BradburyT. N. (2015). Effects of stress on the social support provided by men and women in intimate relationships. Psychological Science, 26(10), 1584–1594. https://doi.org/10.1177/0956797615594616
BrownB. B. (2004). Adolescents’ relationships with peers. In LernerR. M.SteinbergL. (Eds.), Handbook of adolescent psychology (pp. 363–394). John Wiley & Sons.
12.
BuchananT. W.PrestonS. D. (2014). Stress leads to prosocial action in immediate need situations. Frontiers in Behavioral Neuroscience, 8, 5. https://doi.org/10.3389/fnbeh.2014.00005
13.
BurnettS.SebastianC.Cohen KadoshK.BlakemoreS. J. (2011). The social brain in adolescence: Evidence from functional magnetic resonance imaging and behavioural studies. Neuroscience & Biobehavioral Reviews, 35(8), 1654–1664. https://doi.org/10.1016/j.neubiorev.2010.10.011
14.
CannonW. B. (1939). The wisdom of the body (2nd ed.). W W Norton & Company.
15.
CarloG.CrockettL. J.WolffJ. M.BealS. J. (2012). The role of emotional reactivity, self regulation, and puberty in adolescents’ prosocial behaviors. Social Development, 21(4), 667–685. https://doi.org/10.1111/j.1467-9507.2012.00660.x
CeccatoS.KettnerS. E.KudielkaB. M.SchwierenC.VossA. (2018). Social preferences under chronic stress. PLoS One, 13(7), Article e0199528. https://doi.org/10.1371/journal.pone.0199528
19.
CohenS.KamarckT.MermelsteinR. (1983). A global measure of perceived stress. Journal of Health and Social Behavior, 24(4), 385–396. https://doi.org/10.2307/2136404
20.
DaviesD. (2011). Child development: A practitioner’s guide. Guilford Press.
21.
DoK. T.McCormickE. M.TelzerE. H. (2019). The neural development of prosocial behavior from childhood to adolescence. Social Cognitive and Affective Neuroscience, 14(2), 129–139. https://doi.org/10.1093/scan/nsy117
22.
EcclesJ. S.BrownB. V.TempletonJ. (2008). A developmental framework for selecting indicators of well-being during the adolescent and young adult years. In BrownB. (Ed.), Key indicators of child and youth well-being (pp. 197–236). Lawrence Erlbaum.
23.
El‐SheikhM.HargerJ.WhitsonS. M. (2001). Exposure to interparental conflict and children’s adjustment and physical health: The moderating role of vagal tone. Child Development, 72(6), 1617–1636. https://doi.org/10.1111/1467-8624.00369
24.
FeldmanS. S.ElliottG. R. (Eds.), (1990). At the threshold: The developing adolescent. Harvard University Press.
25.
ForbesP. A. G.AydoganG.BraunsteinJ.TodorovaB.WagnerI. C.LockwoodP. L.AppsM. A. J.RuffC. C.LammC. (2024). Acute stress reduces effortful prosocial behaviour. eLife, 12, RP87271. https://doi.org/10.7554/eLife.87271.3
26.
GrantK. E.CompasB. E.ThurmA. E.McMahonS. D.GipsonP. Y. (2004). Stressors and child and adolescent psychopathology: Measurement issues and prospective effects. Journal of Clinical Child and Adolescent Psychology: The Official Journal for the Society of Clinical Child and Adolescent Psychology, American Psychological Association, Division 53, 33(2), 412–425. https://doi.org/10.1207/s15374424jccp3302_23
27.
GrazianoP.DerefinkoK. (2013). Cardiac vagal control and children’s adaptive functioning: A meta-analysis. Biological Psychology, 94(1), 22–37. https://doi.org/10.1016/j.biopsycho.2013.04.011
28.
GuidiJ.LucenteM.SoninoN.FavaG. A. (2021). Allostatic load and its impact on health: A systematic review. Psychotherapy and Psychosomatics, 90(1), 11–27. https://doi.org/10.1159/000510696
29.
HastingsP. D.MillerJ. G. (2014). Autonomic regulation, polyvagal theory, and the development of prosocial behavior. In Padilla-WalkerL.CarloG. (Eds.), Prosocial development: A multidimensional approach (pp. 112–127). Oxford University Press.
30.
HobfollS. E. (1989). Conservation of resources: A new attempt at conceptualizing stress. American Psychologist, 44(3), 513–524. https://doi.org/10.1037/0003-066X.44.3.513
31.
KechterA.BlackD. S.RiggsN. R.WarrenC. M.Ritt-OlsonA.ChouC. P.PentzM. A. (2019). Factors in the perceived stress scale differentially associate with mindfulness disposition and executive function among early adolescents. Journal of Child and Family Studies, 28(3), 814–821. https://doi.org/10.1007/s10826-018-01313-4
32.
KerestešG. (2006). Children’s aggressive and prosocial behavior in relation to war exposure: Testing the role of perceived parenting and child’s gender. International Journal of Behavioural Development, 30(3), 227–239. https://doi.org/10.1177/0165025406066756
33.
KoganA.GruberJ.ShallcrossA. J.FordB. Q.MaussI. B. (2013). Too much of a good thing? Cardiac vagal tone’s nonlinear relationship with well-being. Emotion, 13(4), 599–604. https://doi.org/10.1037/a0032725
34.
KoganA.OveisC.CarrE. W.GruberJ.MaussI. B.ShallcrossA.ImpettE. A.van der LoweI.HuiB.ChengC.KeltnerD. (2014). Vagal activity is quadratically related to prosocial traits, prosocial emotions, and observer perceptions of prosociality. Journal of Personality and Social Psychology, 107(6), 1051–1063. https://doi.org/10.1037/a0037509
35.
KokkaI.ChrousosG. P.DarviriC.BacopoulouF. (2023). Measuring adolescent chronic stress: A review of established biomarkers and psychometric instruments. Hormone Research in Paediatrícs, 96(1), 74–82. https://doi.org/10.1159/000522387
36.
LabordeS.MosleyE.ThayerJ. F. (2017). Heart rate variability and cardiac vagal tone in psychophysiological research–recommendations for experiment planning, data analysis, and data reporting. Frontiers in Psychology, 8, 213. https://doi.org/10.3389/fpsyg.2017.00213
37.
LaibleD.CarloG.MurphyT.AugustineM.RoeschS. (2014). Predicting children’s prosocial and co operative behavior from their temperamental profiles: A person centered approach. Social Development, 23(4), 734–752. https://doi.org/10.1111/sode.12072
38.
LazarusR. S.FolkmanS. (1984). Stress, appraisal, and coping. Springer.
39.
LiZ.SniederH.SuS.DingX.ThayerJ. F.TreiberF. A.WangX. (2009). A longitudinal study in youth of heart rate variability at rest and in response to stress. International Journal of Psychophysiology: Official Journal of the International Organization of Psychophysiology, 73(3), 212–217. https://doi.org/10.1016/j.ijpsycho.2009.03.002
40.
LindholdtL.LabriolaM.AndersenJ. H.KjeldsenM. M. Z.ObelC.LundT. (2022). Perceived stress among adolescents as a marker for future mental disorders: A prospective cohort study. Scandinavian Journal of Public Health, 50(3), 412–417. https://doi.org/10.1177/1403494821993719
MacLeanS. A.AgyemanP. O.WaltherJ.SingerE. K.BaranowskiK. A.KatzC. L. (2019). Mental health of children held at a United States immigration detention center. Social Science & Medicine, 230, 303–308. https://doi.org/10.1016/j.socscimed.2019.04.013
43.
MastenA. S. (2014). Global perspectives on resilience in children and youth. Child Development, 85(1), 6–20. https://doi.org/10.1111/cdev.12205
44.
MastromatteoL. Y.GirardiP.MillerJ. G.ScriminS. (2024). Moderate cardiac vagal tone is associated with more cooperation in children. International Journal of Psychophysiology: Official Journal of the International Organization of Psychophysiology, 201, Article 112371. https://doi.org/10.1016/j.ijpsycho.2024.112371
45.
MastromatteoL. Y.LionettiF.PluessM.ScriminS. (2024). Moderate cardiac vagal tone predicts more cooperation in highly sensitive individuals. Psychophysiology, 61(10), Article e14638. https://doi.org/10.1111/psyp.14638
McLaughlinK. A.AlvesS.SheridanM. A. (2014). Vagal regulation and internalizing psychopathology among adolescents exposed to childhood adversity. Developmental Psychobiology, 56(5), 1036–1051. https://doi.org/10.1002/dev.21187
48.
McLaughlinK. A.Rith-NajarianL.DirksM. A.SheridanM. A. (2015). Low vagal tone magnifies the association between psychosocial stress exposure and internalizing psychopathology in adolescents. Journal of Clinical Child and Adolescent Psychology: The Official Journal for the Society of Clinical Child and Adolescent Psychology, American Psychological Association, Division 53, 44(2), 314–328. https://doi.org/10.1080/15374416.2013.843464
MillerJ. G.KahleS.HastingsP. D. (2017). Moderate baseline vagal tone predicts greater prosociality in children. Developmental Psychology, 53(2), 274–289. https://doi.org/10.1037/dev0000238
51.
MillsK. L.LalondeF.ClasenL. S.GieddJ. N.BlakemoreS. J. (2014). Developmental changes in the structure of the social brain in late childhood and adolescence. Social Cognitive and Affective Neuroscience, 9(1), 123–131. https://doi.org/10.1093/scan/nss113
52.
NitschkeJ. P.SunaharaC. S.CarrE. W.WinkielmanP.PruessnerJ. C.BartzJ. A. (2020). Stressed connections: Cortisol levels following acute psychosocial stress disrupt affiliative mimicry in humans. Proceedings Biological sciences, 287(1927), Article 20192941. https://doi.org/10.1098/rspb.2019.2941
53.
NitschkeJ. P.ForbesP. A.LammC. (2022). Does stress make us more—or less—prosocial? A systematic review and meta-analysis of the effects of acute stress on prosocial behaviours using economic games. Neuroscience & Biobehavioral Reviews, 142, Article 104905. https://doi.org/10.1016/j.neubiorev.2022.104905
54.
Padilla WalkerL. M.DyerW. J.YorgasonJ. B.FraserA. M.CoyneS. M. (2015). Adolescents’ prosocial behavior toward family, friends, and strangers: A person‐centered approach. Journal of Research on Adolescence, 25(1), 135–150. https://doi.org/10.1111/jora.12102
55.
Padilla-WalkerLCarloG (2014). The study of prosocial development: Past, present, and future. In L. Padilla-Walker & G. Carlo (Eds.), Prosocial development: A multidimensional approach (pp. 3–16). Oxford University Press.
56.
PersikeM.Seiffge-KrenkeI. (2014). Is stress perceived differently in relationships with parents and peers? Inter- and intra-regional comparisons on adolescents from 21 nations. Journal of Adolescence, 37(4), 493–504. https://doi.org/10.1016/j.adolescence.2013.09.007
Ribeiro Eulalio CabralG.Rodrigues SampaioL.Ribeiro Eulalio CabralG.SantanaR. B. (2022). Slingshot challenge and star mines: Two digital games as a prisoner’s dilemma to assess cooperation in children. Behavior Research Methods, 54(2), 597–610. https://doi.org/10.3758/s13428-021-01661-y
RosenD. S. (2004). Physiologic growth and development during adolescence. Pediatrics in Review, 25(6), 194–200. https://doi.org/10.1542/pir.25-6-194
61.
ScriminS.MoscardinoU.MasonL. (2019). First graders’ allocation of attentional resources in an emotional stroop task: The role of heart period variability and classroom climate. British Journal of Educational Psychology, 89(1), 146–164. https://doi.org/10.1111/bjep.12228
62.
ShiS.LiB.ShuM.HuaL.ZhouJ.YangQ. (2025). Relations between prosocial behavior and perceived stress in early and middle adolescence and the role of perceived social support and self-esteem as mediators. Child Indicators Research, 18(4), 1–24. https://doi.org/10.1007/s12187-025-10252-8
TaylorL. K.MerrileesC. E.BairdR.Goeke‐MoreyM. C.ShirlowP.CummingsE. M. (2018). Impact of political conflict on trajectories of adolescent prosocial behavior: Im- plications for civic engagement. Developmental Psychology, 54(9), 1785–1794. https://doi.org/10.1037/dev0000552
65.
TaylorS. E. (2006). Tend and befriend: Biobehavioral bases of affiliation under stress. Current Directions in Psychological Science, 15(6), 273–277. https://doi.org/10.1111/j.1467-8721.2006.00451.x
66.
ThayerJ. F.LaneR. D. (2009). Claude bernard and the heart–brain connection: Further elaboration of a model of neurovisceral integration. Neuroscience & Biobehavioral Reviews, 33(2), 81–88. https://doi.org/10.1016/j.neubiorev.2008.08.004
67.
TomovaL.MajdandžićJ.HummerA.WindischbergerC.HeinrichsM.LammC. (2017). Increased neural responses to empathy for pain might explain how acute stress increases prosociality. Social Cognitive and Affective Neuroscience, 12(3), 401–408. https://doi.org/10.1093/scan/nsw146
68.
TomovaL.von DawansB.HeinrichsM.SilaniG.LammC. (2014). Is stress affecting our ability to tune into others? evidence for gender differences in the effects of stress on self-other distinction. Psychoneuroendocrinology, 43, 95–104. https://doi.org/10.1016/j.psyneuen.2014.02.006
VesztegR. F.YamakawaK.MatsubayashiT.UedaM. (2021). Acute stress does not affect economic behavior in the experimental laboratory. PLoS One, 16(1), Article e0244881. https://doi.org/10.1371/journal.pone.0244881
71.
von DawansB.DitzenB.TruegA.FischbacherU.HeinrichsM. (2019). Effects of acute stress on social behavior in women. Psychoneuroendocrinology, 99, 137–144. https://doi.org/10.1016/j.psyneuen.2018.08.031
72.
von DawansB.FischbacherU.KirschbaumC.FehrE.HeinrichsM. (2012). The social dimension of stress reactivity: Acute stress increases prosocial behavior in humans. Psychological Science, 23(6), 651–660. https://doi.org/10.1177/0956797611431576
ZwiK.WoodlandL.WilliamsK.PalasanthiranP.RunganS.JaffeA.WoolfendenS. (2018). Protective factors for social emotional well being of refugee children in the first three years of settlement in Australia. Archives of Disease in Childhood, 103(3), 261–268. https://doi.org/10.1136/archdischild-2016-312495
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