The glycocalyx covering the endothelium is shed during ischemia and reperfusion. The shedding is accompanied by increased levels of the glycocalyx component syndecan-1 in the circulation. Our aim was to compare plasma levels of syndecan-1 in patients undergoing coronary artery bypass grafting (CABG), with or without the use of cardiopulmonary bypass (CPB). Syndecan-1 plasma concentrations were measured in patients undergoing CABG on-pump (n = 22) or off-pump (n = 22). The syndecan-1 concentration increased significantly from 29.5 ± 4.6 ng/mL at baseline to 98.7 ± 9.8 ng/mL (p < 0.01) after the start of CPB or 30 minutes after the induction of anesthesia in the off-pump group. There were no significant differences in peak syndecan-1 plasma concentrations between on-pump and off-pump patients. Plasma levels of syndecan-1 increased significantly during CABG, with or without the use of CPB. There were no significant differences in syndecan-1 concentrations in the two groups.
1 Bignon J Jaubert F Jaurand MC . Plasma protein immunocytochemistry and polysaccharide cytochemistry at the surface of alveolar and endothelial cells in the rat lung. J Histochem Cytochem1976; 24: 1076–1084.
2.
2 Mulivor AW Lipowsky HH . Role of glycocalyx in leukocyte-endothelial cell adhesion. Am J Physiol Heart Circ Physiol2002; 283: H1282–H1291.
3.
3 Adamson RH . Permeability of frog mesenteric capillaries after partial pronase digestion of the endothelial glycocalyx. J Physiol1990; 428: 1–13.
4.
4 Nieuwdorp M van Haeften TW Gouverneur MC . Loss of endothelial glycocalyx during acute hyperglycemia coincides with endothelial dysfunction and coagulation activation in vivo. Diabetes2006; 55: 480–486.
5.
5 Mulivor AW Lipowsky HH . Inflammation- and ischemia-induced shedding of venular glycocalyx. Am J Physiol Heart Circ Physiol2004; 286: H1672–H1680.
6.
6 Rehm M Bruegger D Christ F . Shedding of the endothelial glycocalyx in patients undergoing major vascular surgery with global and regional ischemia. Circulation2007; 116: 1896–1906.
7.
7 Kirklin JK Westaby S Blackstone EH Kirklin JW Chenoweth DE Pacifico AD . Complement and the damaging effects of cardiopulmonary bypass. J Thorac Cardiovasc Surg1983; 86: 845–857.
8.
8 Rothenburger M Trosch F Markewitz A . Leukocyte activation and phagocytotic activity in cardiac surgery and infection. Cardiovasc Surg2002; 10: 470–475.
9.
9 Risnes I Ueland T Lundblad R . Changes in the cytokine network and complement parameters during open heart surgery. Interact Cardiovasc Thorac Surg2003; 2: 19–24.
10.
10 Hoel TN Videm V Mollnes T . Inflammation in on- and off-pump coronary surgery. Perfusion2007; 22: 251–256.
11.
11 Lingaas PS Hol PK Lundblad R . Clinical and radiologic outcome of off-pump coronary surgery at 12 months follow-up: a prospective randomized trial. Ann Thorac Surg2006; 81: 2089–2095.
12.
12 Lingaas PS Hol PK Lundblad R . Clinical and angiographic outcome of coronary surgery with and without cardiopulmonary bypass: a prospective randomized trial. Heart Surg Forum2004; 7: 37–41.
13.
13 Castellheim A Hoel TN Videm V . Biomarker profile in off-pump and on-pump coronary artery bypass grafting surgery in low-risk patients. Ann Thorac Surg2008; 85: 1994–2002.
14.
14 Raja SG Berg GA . Impact of off-pump coronary artery bypass surgery on systemic inflammation: current best available evidence. J Card Surg2007; 22: 445–455.
15.
15 Videm V Fosse E Mollnes TE Garred P Svennevig JL . Time for new concepts about measurement of complement activation by cardiopulmonary bypass. Ann Thorac Surg1992; 54: 725–731.
16.
16 Prondzinsky R Knupfer A Loppnow H . Surgical trauma affects the proinflammatory status after cardiac surgery to a higher degree than cardiopulmonary bypass. J Thorac Cardiovasc Surg2005; 129: 760–766.
17.
17 Alexopoulou AN Multhaupt HA Couchman JR . Syndecans in wound healing, inflammation and vascular biology. Int J Biochem Cell Biol2007; 39: 505–528.
18.
18 Huxley VH Williams DA . Role of a glycocalyx on coronary arteriole permeability to proteins: evidence from enzyme treatments. Am J Physiol Heart Circ Physiol2000; 278: H1177–H1185.
19.
19 Svennevig K Kolset SO Bangstad HJ . Increased syndecan-1 in serum is related to early nephropathy in type 1 diabetes mellitus patients. Diabetologia2006; 49: 2214–2216.
20.
20 Joensuu H Anttonen A Eriksson M . Soluble syndecan-1 and serum basic fibroblast growth factor are new prognostic factors in lung cancer. Cancer Res2002; 62: 5210–5217.
21.
21 Brule S Charnaux N Sutton A . The shedding of syndecan-4 and syndecan-1 from HeLa cells and human primary macrophages is accelerated by SDF-1/CXCL12 and mediated by the matrix metalloproteinase-9. Glycobiology2006; 16: 488–501.
22.
22 Yang Y MacLeod V Miao HQ . Heparinase enhances syndecan-1 shedding: a novel mechanism for stimulation of tumor growth and metastasis. J Biol Chem2007; 282: 13326–13333.
23.
23 Varnous S Ben Ayed S Masliah J . Secretory phospholipase A(2) activity and release kinetics of vascular tissue remodelling biomarkers after coronary artery bypass grafting with and without cardiopulmonary bypass. Clin Chem Lab Med2007; 45: 372–375.
24.
24 Wills BA Oragui EE Dung NM . Size and charge characteristics of the protein leak in dengue shock syndrome. J Infect Dis2004; 190: 810–818.
25.
25 Puskas F Grocott HP White WD Mathew JP Newman MF Bar-Yosef S . Intraoperative hyperglycemia and cognitive decline after CABG. Ann Thorac Surg2007; 84: 1467–1473.
26.
26 Ihrcke NS Platt JL . Shedding of heparin sulfate proteoglycan by stimulated endothelial cells: evidence for proteolysis of cell-surface molecules. J Cell Physiol1996; 168: 625–637.
27.
27 Ofosu FA Modi GJ Blajchman MA Buchanan MR Johnson EA . Increased sulphation improves the anticoagulant activities of heparan sulphate and dermatan sulphate. Biochem J1987; 248: 889–896.
28.
28 Henry CB Duling BR . TNF-alpha increases entry of macromolecules into luminal endothelial cell glycocalyx. Am J Physiol Heart Circ Physiol2000; 279: H2815–H2823.
29.
29 Kuschert GS Coulin F Power CA . Glycosaminoglycans interact selectively with chemokines and modulate receptor binding and cellular responses. Biochemistry1999; 38: 12959–12968.
30.
30 Parolari A Camera M Alamanni F . Systemic inflammation after on-pump and off-pump coronary bypass surgery: a one-month follow-up. Ann Thorac Surg2007; 84: 823–828.
31.
31 Johansson-Synnergren M Nilsson F Bengtsson A Jeppsson A Wiklund L . Off-pump CABG reduces complement activation but does not significantly affect peripheral endothelial function: a prospective randomized study. Scand Cardiovasc J2004; 38: 53–58.
32.
32 Khan NE De Souza A Mister R . A randomized comparison of off-pump and on-pump multivessel coronary-artery bypass surgery. N Engl J Med2004; 350: 21–28.
