Evaluation of the Immunotoxicity of Antiretroviral Drugs Using an In Vitro Method for the Induction and Measurement of a Specific Antibody Response in Human Peripheral Blood Lymphocytes
Restricted accessResearch articleFirst published online March, 1995
Evaluation of the Immunotoxicity of Antiretroviral Drugs Using an In Vitro Method for the Induction and Measurement of a Specific Antibody Response in Human Peripheral Blood Lymphocytes
We studied the effects of the antiretroviral drugs 3’-azido-3’-deoxythymidine (AZT) and 2’,3’-dideoxycytidine (DDC) on the induction of a specific primary antibody response to the antigen SRC and on Pokeweed mitogen-induced polyclonal immunoglobulin (Ig) production in cultures of normal human peripheral blood lymphocytes.
The results presented here show that AZT and DDC inhibited both systems in a dose-dependent way. However, inhibition of the antigen-specific antibody response occurred at drug concentrations significantly lower than those effective on the mitogen-driven polyclonal response. This was particularly striking for DDC, which was approximately 100 times more powerful, on a molar basis, on the specific antibody response than on polyclonal Ig production. Thus, in vitro systems for the induction of antigen-specific antibody responses in human lymphocytes may be important, sensitive tools for preclinical studies of the immunotoxicity of antiretroviral drugs.
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