In this study, the isolated perfused gills of rainbow trout (Oncorhynchus mykiss) provided a tool for fundamental research on the rate-limiting step in the uptake of organic hydrophobic chemicals. Data obtained using the isolated gills were comparable to those determined in vivo. Whereas several tens of fish need to be used to obtain statistically sound information on uptake rates in vivo, the isolated perfused gills method requires only three or four fish. A significant reduction in animal use is thus obtained.
GobasF.A.P.C., OpperhuizenA., and HutzingerO. (1986). Bioconcentration of hydrophobic chemicals in fish: relationship with membrane permeation.Environmental Toxicology and Chemistry5, 637–646.
2.
MackayD. (1982). Correlation of bioconcentration factors.Environmental Science and Technology11, 475–478.
3.
Anon (1981). Guidelines for Testing of Chemicals. Nos 107, 116 and 305. Paris: OECD.
4.
OpperhuizenA. (1986). Bioconcentration of hydrophobic chemicals in fish. In Aquatic Toxicology and Environmental Fate, Vol. 9, ASTM STP 921 (ed. PostonT.M., PurdyR.), pp. 304–315. Philadelphia: American Society for Testing and Materials.
5.
PartP. (1990). The perfused gill preparation in studies of the bioavailability of chemicals.Ecotoxicology and Environmental Safety19, 106–115.
6.
SijmD.T.H.M., PärtP., and OpperhuizenA. (1993). The influence of temperature on the uptake of hydrophobic compounds determined by the isolated perfused gills of rainbow trout (Oncorhynchus mykiss).Aquatic Toxicology25, 1–14.
7.
PartP., and SvanbergO. (1981). Uptake of cadmium in perfused rainbow trout (Salmo gairdneri) gills.Canadian Journal of Fisheries and Aquatic Sciences38, 917–924.
8.
BransonD.R., BlauG.E., AlexanderH.C., and NeelyW.B. (1975). Bioconcentration of 2,2’,4,4’-tetrachlorobiphenyl in rainbow trout as measured by an accelerated test.Transactions of the American Fisheries Society104, 785–792.
9.
OpperhuizenA., & SijmD.T.H.M. (1990). Bio-accumulation and biotransformation of poly-chlorinated dibenzo-p-dioxins and dibenzofurans in fish.Environmental Toxicology and Chemistry9, 175–186.
10.
PartP., SaarikoskiJ., TuurulaH., and HavasteH. (1992). The absorption of hydrophobic chemicals across perfused rainbow trout gills: methodological aspects.Ecotoxicology and Environmental Safety24, 275–286.
11.
PartP., TuurulaH., NikinmaaM., and KiesslingA. (1984). Evidence for a non-respiratory intra-lamellar shunt in perfused rainbow trout gills.Comparative Biochemistry and Physiology79A, 29–34.
12.
OpperhuizenA., and VoorsP.I. (1987). Uptake and elimination of polychlorinated aromatic ethers by fish: chloroanisoles.Chemosphere16, 953–962.
13.
De VoogtW.P. (1990). QSARs for the environmental behaviour of polynuclear (hetero) aromatic compounds: studies in aquatic systems. Ph.D. Thesis, 258 pp., Free University of AmsterdamAmsterdam.
