The acute toxicities of the first 50 chemicals (dextropropoxyphene hydrochloride excluded) of the multicentre evaluation of in vitro cytotoxicity (MEIC) programme were determined on four aquatic invertebrates and a bacterial strain (Photobacterium phosphoreum for the Microtox™ test) commonly used in ecotoxicology testing. Three of the aquatic invertebrate tests consisted of cyst-based toxicity tests (Artoxkit M with Artemia salina, Streptoxkit F with Streptocephalus proboscideus, and Rotoxkit F with Brachionus calyciflorus), and the Daphnia magna test. Results of simple linear regression analyses indicated that the rodent tests (rat and/or mouse) were better than the ecotoxicological tests for predicting acute oral lethal doses in man. However, it appears that the batteries of ecotoxicological tests resulting from the partial least squares method appear to be better than the rodent tests for predicting human oral lethal doses.
EkwallB. (1980a). Preliminary studies on the validity of in vitro measurement of drug toxicity using HeLa cells. II. Drug toxicity in the MIT-24 system compared with mouse and human lethal dosage of 52 drugs.Toxicology Letters5, 309–317.
2.
EkwallB. (1980b). Preliminary studies on the validity of in vitro measurement of drug toxicity using HeLa cells. III. Lethal action to man of 43 drugs related to the HeLa cell toxicity of the lethal drug concentrations.Toxicology Letters5, 319–331.
3.
EkwallB., & JohanssonA. (1980). Preliminary studies on the validity of in vitro measurement of drug toxicity using HeLa cells. I. Comparative in vitro cytotoxicity of 27 drugs.Toxicology Letters5, 299–307.
4.
EkwallB., & AcostaD. (1982). In vitro comparative toxicity of selected drugs and chemicals in HeLa cells, Chang liver cells, and rat hepatocytes.Drug and Chemical Toxicology5, 219–231.
5.
EkwallB. (1983). Screening of toxic compounds in mammalian cell cultures.Annals of the New York Academy of Sciences407, 64–75.
6.
WalumE., & PetersonE. (1984). On the application of cultured neuroblastoma cells in chemical toxicity screening.Journal of Toxicology and Environmental Health13, 511–520.
7.
BorenfreundE., & ShopsisC. (1985). Toxicity monitored with a correlated set of cell culture assays.Xenobiotics15, 705–711.
8.
Gómez-LechónM.J., MontoyaA., LopezP., DonatoT., LarrauriA., & CastellJ.V. (1988). The potential use of cultured hepatocytes in predicting the hepatotoxicity of xenobiotics.Xenobiotica18, 725–735.
9.
GoldbergA.M., & FrazierJ.M. (1989). Alternatives to animals in toxicity testing.Scientific American261, 16–22.
10.
EkwallB., BondessonI., CastellJ.V., Gómez-LechónM.J., HellbergS., HögbergJ., JoverR., PonsodaX., RomertL., StenbergK., & WalumE. (1989). Cytotoxicity evaluation for the first ten MEIC chemicals: acute lethal toxicity in man predicted by cytotoxicity in five cellular assays and by oral LD50 in rodents.ATLA17, 83–100.
11.
FryJ.R., GarleM.J., HammondA.H., & HatfieldA. (1990). Correlation of acute lethal potency with in vitro cytotoxicity.Toxicology in Vitro4, 175–178.
12.
WeissM.T., & SawyerT.W. (1993). Cytotoxicity of the MEIC test chemicals in primary neuron cultures.Toxicology in Vitro, in press.
13.
FiskesjöG. (1985). The Allium test as a standard in environmental monitoring.Hereditas102, 99–112.
14.
KapplerR., & KristenU. (1987). Photometric quantification of in vitro pollen tube growth: a new method suited to determine the cytotoxicity of various experimental substances.Environmental and Experimental Botany27, 305–309.
15.
CallejaM.C., & PersooneG. (1992). Cyst-based toxicity tests. IV. The potential of ecotoxicological tests for the prediction of acute toxicity in man as evaluated on the first ten chemicals of the MEIC programme.ATLA20, 396–405.
16.
KersterH.W., & SchaefferD.J. (1983). Brine shrimp (Artemia salina) nauplii as a teratogen test system.Ecotoxicology and Environmental Safety7, 342–349.
17.
GossL.B., FaulkR.T., ReveleyJ.W., & SabourinT.D. (1986). Utilization of non-mammalian test systems to predict human health hazards. In Aquatic Toxicology and Environmental Fate, Vol. IX (ed. PostonT.M., & PurdyR.), pp. 316–331. Philadelphia, PA, USA: American Society for Testing and Materials.
18.
SilvermanJ. (1983). Preliminary findings on the use of protozoa (Tetrahymena thermophila) as models for ocular irritation testing in rabbits.Laboratory Animal Science33, 56–59.
19.
GossL.B., & SabourinT.D. (1985). Utilization of alternative species for toxicity testing: an overview.Journal of Applied Toxicology5, 193–219.
20.
SilvermanJ., & PennisiS. (1987). Evaluation of Tetrahymena thermophila as an in vitro alternative to ocular irritation studies in rabbits.Journal of Toxicological Cutaneous and Ocular Toxicology6, 33–42.
21.
AhlersJ., BenzigM., GiesA., PauliW., & RösickE. (1988). Yeast as a unicellular model system in ecotoxicology and xenobiochemistry.Chemosphere17, 1,603–1,615.
22.
StadtlanderK., & LawohnusH. (1990). Yeast cells as a test system for evaluating the toxicity of chemicals.ATLA17, 203–206.
23.
WilliamsG.L. (1980). Batteries of short-term tests for carcinogen screening. In The Predictive Screening Tests in Carcinogenicity Evaluation (ed. WilliamsG.M., KroesR., WaaijersH.W., & Van de PollK.W.), pp. 327–346. Amsterdam: Elsevier.
24.
TosatoM.L., CesareoD., PasseriniL., & ClementiS. (1988). PLS assessment of the performance of short-term tests for carcinogens.Journal of Chemometrics2, 171–187.
25.
BrookesP., & de SerresF.J. (1981). Overview of assay system performance. In Evaluation of Short-term Tests for Carcinogens: Progress in Mutation Research, Vol. I (ed. de SerresF.J., & AshbyJ.), pp. 110–111. New York: Elsevier.
26.
BondessonI., EkwallB., HellbergS., RomertL., StenbergK., & WalumE. (1989). MEIC: a new international multicentre project to evaluate the relevance to human toxicity of in vitro cytotoxicity tests.Cell Biology and Toxicology5, 331–347.
27.
VanhaeckeP., PersooneG., ClausC., & SorgeloosP. (1980). Research on the development of a short-term standard toxicity test with Artemia nauplii. In The Brine Shrimp, Vol. I, Morphology, Genetics, Radiobiology, Toxicology (ed. PersooneG., SorgeloosP., RoelsO., & JaspersE.), pp. 263–285. Wetteren, Belgium: Universa Press.
28.
VanhaeckeP., & PersooneG. (1981). Report on an intercalibration exercise on a short-term standard toxicity test with Artemia nauplii (ARC test). In Les Tests de Toxicité Aigue en Milieu Aquatique, Les Colloques de l'INSERM, Vol. CVI (ed. LeclercH., & DiveD.), pp. 359–376. Paris: INSERM.
29.
SnellT.W., & PersooneG. (1989). Acute toxicity bioassays using rotifers. II. A freshwater test with Brachionus rubens.Aquatic Toxicology14, 81–92.
30.
CentenoM.D.F., BrendonckL., & PersooneG. (1993). Cyst-based toxicity tests. III. Development and standardization of an acute toxicity test with the freshwater anostracan Streptocephalus proboscideus. In Progress in Standardization of Aquatic Toxicity Tests (ed. SoaresA.M.V.M., & CalowP.). Boca Raton, FL, USA: CRC Press, pp. 37–55.
31.
Anon., (1984). Daphnia species: acute immobilization test and reproduction test. In OECD Guidelines for Testing of Chemicals202, 1–16. Paris, OECD.
32.
Anon., (1989). A Microtox Manual. How to Run Toxicity Tests Using the Microtox Model 500, 41 pp. Carlsbad, CA, USA: Microbics.
33.
Anon., (1984). Registry of Toxic Effects of Chemical Substances, US Department of Health and Human Services Publication. 1,655 pp. Cincinnati, OH, USA: NIOSH.
34.
Anon., (1991). Registry of Toxic Effects of Chemical Substances, US Department of Health and Human Services Publication. 1,731 pp. Cincinnati, OH, USA: NIOSH.
35.
EllenhornM.J., & BarcelouxD.G. (1988). Medical Toxicology Diagnosis and Treatment of Human Poisoning, 1,512 pp. New York: Elsevier.
36.
BaseltR.C., & CraveyR.H. (1989). Disposition of Toxic Drugs and Chemicals in Man, 3rd edn, 875 pp. Chicago, IL, USA: Year Book Medical Publishers.
37.
HamiltonM.A., RussoR.C., & ThurstonR.V. (1977). Trimmed Spearman-Karber method for estimating median lethal concentrations in toxicity bioassays.Environmental Science and Technology11, 714–719 (correction 12, 417).
38.
SokalR.R., & RohlfF.J. (1981). Biometry, 2nd edn, 859 pp. New York: W.H. Freeman.
39.
HellbergS., ErikssonL., JonssonJ., LindgrenF., SjöströmM., WoldS., EkwallB., Gómez-LechónM.J., ClothierR., AccomandoN.J., GrimesA., BarileF.A., NordinM., TysonC.A., DierickxP., ShrivastavaR.S., Tingsleff-SkaanildM., Garza-Ocan¯asL., & FiskesjöG. (1990). Analogy models for prediction of human toxicity.ATLA18, 103–116.
40.
WoldD., RuheA., WoldH., & DunnW.J.3rd (1984). The colinearity problem in linear regression. The partial least squares (PLS) approach to generalised inverses.SIAM Journal of Science and Statistical Computation5, 735–742.
41.
GeladiP., & KowalskiB.R. (1986). Partial least square regression: a tutorial.Analytica Chimica Acta185, 1–17.
42.
GeladiP., & KowalskiB.R. (1986b). An example of 2-block predictive partial least squares regression with simulated data.Analytica Chimica Acta185, 19–32.
43.
WoldS. (1978). Cross-validatory estimation of the number of components in factor and principal component models.Technometrics20, 397–405.
44.
CramerR.D.3rd, BunceJ.D., PattersonD.E., & FrankI.E. (1988). Cross-validation, boot-strapping and partial least squares compared with multiple regression in conventional QSAR studies.Quantitative Structure-Activity Relationships7, 18–25.
45.
BrodieB.B., & ReidW.D. (1967). Some pharmacological consequences of species variation in rates of metabolism.Federation Proceedings26, 1,062–1,070.
46.
ElmamloukT.H., & GessnerT. (1976). Species differences in metabolism of parathion: apparent inability of hepatopancreas fraction to produce paraoxon.Comparative Biochemistry and Physiology53C, 19–24.
47.
SmithJ.N. (1977). Comparative detoxification of invertebrates. In Drug Metabolism: From Microbe to Man (ed. ParkeD.V., & SmithR.L.), pp. 219–230. London: Taylor & Francis.
48.
BendJ.R., & JamesM.O. (1978). Xenobiotic metabolism in marine and freshwater species. In Biochemical and Biophysical Perspectives in Marine Biology, Vol. IV (ed. MalinsD.C., & SargentJ.R.), pp. 125–187. London: Academic Press.
49.
FosterG.D., & CrosbyD.G. (1987). Comparative metabolism of nitroaromatic compounds in freshwater, brackish water and marine decapod crustaceans.Xenobiotica17, 1,393–1,404.
50.
RueliusH.W. (1987). Extrapolation from animals to man: prediction, pitfalls and perspectives.Xenobiotica17, 255–265.
51.
BarronM.G., & PlakasS.M. (1991). Pharmacokinetics and bioavailability of xenobiotics in aquatic animals. In SETAC 12th Annual Meeting, 1991, 280 pp. Washington, DC: SETAC.
52.
KukkonenJ., & OikariA. (1988). Sulfate conjugation is the main route of pentachlorophenol metabolism in Daphnia magna. Comparative Biochemistry and Physiology91C, 465–468.
