A multicentre validation study of the acute in vitro cytotoxicities of 31 liquid or solid chemicals was carried out by six laboratories, using primary rat hepatocyte cultures as a model system. We report here a comparison of neutral red uptake IC50 and LD50 values. Oral, i.p. and i.v. LD50 values were available for 27, 24 and 18 chemicals, respectively, and an IC50 value was obtained for 15, 14 and 11 of these compounds, respectively. A significant correlation was found only between IC50 and i.v. LD50 values.
Get full access to this article
View all access options for this article.
References
1.
ClothierR.H., HulmeL.M., SmithM., and BallsM. (1987). Comparison of the in vitro cytotoxicities arid acute in vivo toxicities of 59 chemicals.Molecular Toxicology1, 571–577.
2.
EkwallB. (1983). Correlation between cytotoxicity in vitro and LD50 values.Acta Pharmacologica et Toxicologica52, Suppl. 2, 80–99.
3.
FautrelA., ChesnéC., GuillouzoA., De SousaG., PlacidiM., RahmaniR., BrautF., PichonJ., HoellingerH., VintezouP., DiarteI., MelcionC., CordierA., LorenzonG., BenicourtM., VannierB., FournexR., PelouxA.F., BichetN., GouyD., CanoJ.P., and LounesR. (1991). A multicentre study of acute in vitro cytotoxicity in rat liver cells.Toxicology in Vitro5, 543–547.
4.
FryJ.R., GarleM.J., HammondA.H., and HatfieldA. (1990). Correlation of acute lethal potency with in vitro toxicity.Toxicology in Vitro4, 175–179.
5.
PelouxA-F., FedericiC., BichetN., GouyD., & CanoJ-P. (1992). Hepatocytes in primary culture: an alternative to LD50 testing? Validaton of a predictive model by multivariate analysis.ATLA20, 8–26.
