Various cell culture models have been proposed as partial or total replacements for in vitro ocular irritation tests. The cytotoxic potential of substances can be evaluated according to various parameters for measuring cellular viability and metabolic capacity. Cell cultures have their limitations, but they also have advantages which justify the inclusion of some such models in a battery of in vitro tests that could replace the Draize test.
FrazierJ.M. (1990). Scientific criteria for validation of in vitro toxicity tests. Environment Monographs. no. 36, 62 pp. Paris: OECD.
2.
GadS.C. (1989). Acute ocular irritation evaluation: in vivo and in vitro alternatives and making them the “standard” for testing. In Benchmarks: Alternative Methods in Toxicology (ed. MehlmanM.A.), pp. 137–193. Princeton, NJ: Princeton Scientific.
3.
BorenfreundE., and BorreroO. (1984). In vitro cytotoxicity assays: potential alternatives to the Draize ocular irritancy test.Cell Biology and Toxicology1, 33–39.
4.
KnoxP., UphillJ.R., FryJ.R., BenfordD.J., and BallsM. (1986). The FRAME multicentre project on in vitro cytotoxicology.Food and Chemical Toxicology24, 457–463.
5.
BorenfreundE., and PuernerA. (1984). A simple quantitative procedure using monolayer cultures for cytotoxicity assays (HTD/NR-90).Journal of Tissue Culture Methods9, 7–9.
6.
ScaifeM.C. (1982). An investigation of detergent action on cells in vitro and possible correlations with in vivo data.International Journal of Cosmetic Science4, 179–193.
7.
MarinovichM., TraguiE., CorsiniA., and GalliC. (1990). Quantification of in vitro cytotoxicity of surfactants: correlation with their eye irritant potential.Journal of Toxicology Cutaneous and Ocular Toxicology9, 169–178.
8.
ShadduckJ.A., EverittJ., and BayP. (1985). Use of in vitro cytotoxicity to rank ocular irritation of six surfactants. In Alternative Methods in Toxicology, Vol. 1, In Vitro Methods in Toxicology (ed. GoldbergA.M.), pp. 641–649. New York: Mary Ann Liebert.
9.
ReaderS.J., BlackwellV., O'HaraR., ClothierR.H., GriffinG., and BallsM. (1989). A vital dye release method for assessing the short-term cytotoxic effects of chemicals and formulations.ATLA17, 28–33.
10.
TchaoR. (1988). Trans-epithelial permeability of fluoresceine in vitro as an assay to determine eye irritant.Alternative Methods in Toxicology, Vol. 6, Progress in In Vitro Toxicology (ed. GoldbergA.M.), pp. 281–283. New York: Mary Ann Liebert.
11.
North-RootH., YackovichF., DemetruliasJ., GaculaM., and HeinzeJ.E. (1982). Evaluation of an in vitro cell toxicity test using rabbit corneal cells to predict the eye irritation potential of surfactants.Toxicology Letters14, 207–212.
12.
BrunerL.H., MillerK.R., OwickiJ.C., ParceJ.W., and MuirV.C. (1991). Testing ocular irritancy in vitro with the silicon microphysiometer.Toxicology in Vitro5, 277–284.
13.
ChanK.Y. (1986). Chemical injury to an in vitro ocular system: differential release of plasminogen activator.Current Eye Research5, 358–362.
14.
WilcoxD.K., and BrunerL.H. (1990). In vitro alternatives for ocular safety testing: an outline of assays and possible future developments.ATLA18, 117–128.
15.
JumblattM.M., and NeufelA.H. (1986). A tissue culture assay of corneal epithelial wound closure.Investigative Ophthalmology and Visual Science27, 813.
16.
GriffithJ.F., NixonG.A., BruceR.D., ReerP.J., and BannanE.A. (1980). Dose-response studies with chemical irritants in the albino rabbit eye as a basis for selecting optimum testing conditions for predicting hazard to the human eye.Toxicology and Applied Pharmacology55, 501–513.
17.
SeifreidH.E. (1986). Eye irritation testing: historical perspectives and future directions.Journal of Toxicology — Cutaneous and Ocular Toxicology5, 89–114.
18.
GuillotR. (1991). Cell cultures Complementary or alternative methods to experiments on animals.Journal de Pharmacie de Belgique46, 197–200.
19.
ZbindenG. (1990). In vitro methods, past successes and future challenges.Acta Physiologica Scandinavica, Suppl. 592, 9–12.
