This paper describes the application of cell culture to the investigation of adverse effects associated with continuous ambulatory peritoneal dialysis. Detailed description is given of effects caused by reactive oxygen species, such as could be generated in infections, on confluent sheets of MDCK cells. The electrical effect was reduction in transmonolayer resistance. Various morphological abnormalities were concurrently observed. The method has applications in other barrier membrane studies.
JabarZ., and DawsonM. (1989). Effect of chlorhexidine on Madin-Darby canine kidney cells.Molecular Toxicology1, 445–452.
2.
MadinS.H., and DarbyN.B. (1958). Established kidney cell line of normal bovine and ovine origin.Proceedings of the Society for Experimental Biology and Medicine98, 573–576.
3.
DrukkerW., ParsonsF.M., and MaherJ.F. (1986). Replacement of Renal Function by Dialysis, Vol. II. Lancaster: Martinus Nijhoff.
4.
HarlanJ.M., SchwartzB.R., ReidyM.A., SchwartzS.M., OchsH.D., and HarkerL.A. (1985)! Activated neutrophils disrupt endothelial monolayer integrity by an oxygen radical-independent mechanism.Laboratory Investigation52, 141–150.
