Cytotoxic and Enzyme-Inducing Effects of Rodent Diets and Cage Bedding Materials: Evaluation by a Cell Culture Study

Abstract

Several rodent diets and wood-based materials used as cage bedding for laboratory animals were studied for their cytotoxic and cytochrome P450-inducing properties by an in vitro method. The cell culture system using a mouse hepatoma cell line (Hepa-1) proved to be a convenient and sensitive screening method, and considerable differences among both the feeds and the bedding materials were detected.

Keywords

diet feed bedding enzyme induction cytotoxicity cell culture aryl hydrocarbon hyroxylase cytochrome P450 in vitro toxicity

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