A comparison of data from assays performed in cells of an established line derived from a human hepatoblastoma (Hep G2) with those previously obtained in freshly-isolated human hepatocytes, reveals that the latter are, by varying degrees, more sensitive than the former to the cytotoxic and genotoxic effects of 10 N-nitroso compounds, independently of their nature as proximate or ultimate carcinogens.
HsuI., HarrisC.C., LipskyM.M., SnyderS., and TrumpB.F. (1987). Cell and species differences in metabolic activation of chemical carcinogens.Mutation Research177, 1–7.
2.
MartelliA., RobbianoL., GazzanigaG.M., and BrambillaG. (1988). Comparative study of DNA damage and repair induced by 10 N-nitroso compounds in primary cultures of human and rat hepatocytes.Cancer Research48, 4144–4152.
3.
ButterworthB.E., Smith-OliverT., EarleL., LouryD.J., WhiteR.D., DoolittleD.J., WorkingP.K., CattleyR.C., JirtleR., MichalopoulosG., and StromS. (1989). Use of primary cultures of human hepatocytes in toxicology studies.Cancer Research49, 1075–1084.
4.
KnowlesB.B., HoweC.C., and AdenD.P. (1980). Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen.Science209, 497–499.
5.
DiamondL., KruszewskiF., AdenD.P., KnowlesB.B., and BairdW.M. (1980). Metabolic activation of benzo[a]pyrene by a human hepatoma cell line.Carcinogenesis1, 871–875.
6.
DearfieldK.L., Jacobson-KramD., BrownN.A., and WilliamsJ.R. (1983). Evaluation of a human hepatoma cell line as a target cell in genetic toxicology.Mutation Research108, 437–449.
7.
DearfieldK.L., Jacobson-KramD., HuberB.E., and WilliamsJ.R. (1986). Induction of sister chromatid exchanges in human and rat hepatoma cell lines by cyclophosphamide and phosphoramide mustard and the effects of cytochrome P450 inhibitors.Biochemical Pharmacology35, 2199–2205.
8.
EddyE.P., HowardP.C., McCoyG.D., and RosenkranzH.S. (1987). Mutagenicity, unscheduled DNA synthesis, and metabolism of 1-nitropyrene in the human hepatoma cell line Hep G2.Cancer Research47, 3163–3168.
9.
BartschH., and MontesanoR. (1984). Relevance of nitrosamines to human cancer.Carcinogenesis5, 1381–1393.
10.
International Agency for Research on Cancer (1987). The Relevance of N-Nitroso Compounds to Human Cancer: Exposure and Mechanisms. In IARC Scientific Publication No. 84 (ed. BartschH., O'NeillI.K., & Schulte-HermannR.). Lyon: International Agency for Research on Cancer.
11.
LaishesB.A., RobertsE., and FarberE. (1978). In vitro measurement of carcinogen-resistant liver cells during hepatocarcinogenesis.International Journal of Cancer21, 186–193.
12.
ArgusM.F., and ArcosJ.C. (1978). Use of high concentrations of dimethyl nitrosamine in bacterial lethality, mutagenesis and enzymological studies.Cancer Research38, 226–228.
13.
GuttenplanJ.B. (1987). N-nitrosamines: bacterial mutagenesis and in vitro metabolism.Mutation Research186, 81–134.
14.
Cavalli-SforzaL. (1980). Analisi Statistica per Medici e Biologi175 pp. Torino: Boringhieri.
15.
RümkeC.L., & De JongeH. (1964). Design, statistical analysis and interpretation. In Evaluation of Drug Activities: Pharmacometrics, Vol. I (ed. LaurenceD.R., BacharachA.L.), pp. 80–82. New York: Academic Press.