The effects of methylmercury (MeHg) and triethyllead (Et3Pb) were tested on the development of oligodendroglia cells (OG) in primary glia-rich cultures of rat brain cells. The marker enzyme 2'3’-cyclic nucelotide 3’-phosphodiesterase (CNP) was used as an index of OG development. The increase in CNP was followed during 1–4 weeks of exposure. MeHg at 2.5x107 and 10 8M, and Et3Pb at 2.5x10 7M, markedly inhibited the age-dependent increase in CNP shown in control cultures. CNP was highly stimulated by 2.5x10-8M Et3Pb. These observations are discussed in relation to results from experiments in vivo on CNP activities after toxic exposure.
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References
1.
WigginsR.C. (1982). Myelin development and nutritional insufficiency.Brain Research Reviews4, 151–175.
2.
RaffM.C., and MillerR.H. (1984). Glial cell development in the rat optic nerve.Trends in Neurological Sciences7, 469–472.
3.
GrundtI.K. (1986). Adenine nucleotides in cultured brain cells after exposure to methyl mercury and triethyl lead.Acta Pharmacologica et Toxicologica59, 11–16.
4.
LabourdetterG., RousselG., and NussbaumJ.L. (1980). Oligodendroglia content of glial cell primary cultures from newborn rat brain hemispheres, depends on the initial plating density.Neuroscience Letters18, 203–275.
5.
SoginD.C. (1976). 2'3’-cyclic NADP as a substrate for 2'3’-cyclic nucleotide 3’-phosphohydrolase.Journal of Neurochemistry27, 1333–1337.
6.
GrundtI.K., and NeskovicN.M. (1985). UDP galactose: ceramide galactosyltransferase and 2'3’-cyclic nucleotide 3’-phosphodiesterase activities in rat brain after long-term exposure to methylmercury and triethyllead.Experimental Neurology88, 580–589.
