The Toxic Effects of Two Dental Materials on Human Buccal Epithelium In Vitro and Monkey Buccal Epithelium In Vivo

Abstract

The aim of the present study was to compare the toxicity in vitro with the toxicity in vivo of two commercial chemicals marketed for use in the oral cavity (GLUMA Bond^R and 3M Etching Liquid^R).

Confluent cultures of human buccal epithelial cells were exposed to graded concentrations of GLUMA Bond or 3M Etching Liquid for 5 minutes. The cytotoxic effects induced by this treatment were observed (cytomorphology, proliferation rate).

In vivo, monkey buccal epithelium was exposed to GLUMA Bond or 3M Etching Liquid for 5 minutes. Biopsies were taken after 24 hours, and the buccal epithelium processed for light microscopical examination.

In both models, the toxic reactions to GLUMA Bond were far more extensive than those caused by 3M Etching Liquid.