The cytotoxicity of vanadate was tested in CHO-cells using uptake of neutral red (NR) and the MIT test as indicators. Cells were exposed to 1–500μM monovanadate for 2–72 hours. Cells exposed to 100μM monovanadate caused a 50% reduction in MIT activity measured after 30 hours, while a similar reduction in NR uptake was observed after 16 hours exposure to the same vanadate concentration. Monovanadate also caused a reduction in amount of cellular protein; a 50% reduction was observed after 24 hours exposure to 100μM monovanadate.
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