Real progress has been made towards replacing animals for risk assessment purposes, particularly given the recent announcement of comprehensive roadmaps by regulatory agencies. However, given the vast number of animals that continue to be used for biomedical purposes, perhaps now is the time to place greater emphasis on replacing animals in biomedical research. Scientific journals have a key role to play in encouraging the uptake of new approach methodologies and providing a supportive environment for a cultural shift to humane, sustainable Three Rs-based science.
KinterLBDeHavenRJohnsonDK, et al.A brief history of use of animals in biomedical research and perspective on non-animal alternatives. ILAR J2021; 62: 7–16.
2.
WoodABreffaCChaineC, et al.Next generation risk assessment for occupational chemical safety — A real world example with sodium-2-hydroxyethane sulfonate. Toxicology2024; 506: 153835.
3.
SewellFAggarwalMBachlerG, et al.The current status of exposure-driven approaches for chemical safety assessment: A cross-sector perspective. Toxicology2017; 389: 109–117.
MarshallLJBaileyJCassottaM, et al.Poor translatability of biomedical research using animals — A narrative review. Altern Lab Anim2023; 51: 102–135.
10.
AbarkanFZWijenAMAvan EijdenRMG, et al.Identifying key factors for accelerating the transition to animal-testing-free medical science through co-creative, interdisciplinary learning between students and teachers. Animals2022; 12: 2757.
11.
KaplanBLFHobermanAMSlikkerWJr, et al.Protecting human and animal health: The road from animal models to new approach methods. Pharmacol Rev2024; 76: 251–266.
DeckhaMMichelMAzilagbetorD, et al.Accelerating animal replacement: How universities can lead — Results of a one-day expert workshop in Zurich, Switzerland. Altern Lab Anim2025; 53: 106–118.
17.
European Commission. Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes. Off J Euro Union L2762010: 33–79.
GriffinGLockeP. Comparison of the Canadian and US laws, regulations, policies, and systems of oversight for animals in research. ILAR J2016; 57: 271–284.
20.
DukesJPBealeACampC. Reviewing current guidance for the ‘R’ of Replacement and rethinking it with the ‘Replacement Checklist’. Altern Lab Anim2025; 53: 72–83.
RobinsonSSparrowSWilliamsB, et al.The European Federation of the Pharmaceutical Industry and Associations’ Research and Animal Welfare Group: Assessing and benchmarking ‘Culture of Care’ in the context of using animals for scientific purpose. Lab Anim2019; 54: 23677219887998.
23.
AmeliKKrämerS. Transforming culture: A culture of care in animal research. Soc Anim2024; 1–20.
24.
KrebsCELamAMcCarthyJ, et al.A survey to assess animal methods bias in scientific publishing. ALTEX2023; 40: 665–676.
25.
KorevaarDALeenaarsMSterkPJ, et al.Publication bias in laboratory animal research: A survey on magnitude, drivers, consequences and potential solutions. PLoS One2012; 7: e43404.
26.
JooberRSchmitzNAnnableL, et al.Publication bias: What are the challenges and can they be overcome?J Psychiatry Neurosci2012; 37: 149–152.
27.
MaddenJCKandarovaHNeuhausW, et al.Journeying through journals: The publishing process and how to maximise research impact. Altern Lab Anim2024; 52: 334–351.
28.
HendersonVCDemkoNHakalaA, et al.A meta-analysis of threats to valid clinical inference in preclinical research of sunitinib. eLife2015; 4: e08351.
29.
OsborneNAveyMTAnestidouL, et al.Improving animal research reporting standards: HARRP, the first step of a unified approach by ICLAS to improve animal research reporting standards worldwide. EMBO Rep2018; 19: e46069.
30.
KitsaraMSmajlhodžić-DeljoMGurbeta PokvicL, et al.Introducing the COST Action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE). Altern Lab Anim2024; 52: 326–333.
31.
MohapatraRLeistMvon AulockS, et al.Guidance for Good In Vitro Reporting Standards (GIVReSt) — A draft for stakeholder discussion and background documentation. ALTEX2025; 42: 376–396.
32.
Percie du SertNHurstVAhluwaliaA, et al.The ARRIVE guidelines 2.0: Updated guidelines for reporting animal research. PLoS Biol2020; 18: e3000410.
