Anon. (2005). Report of the Joint FAO/IOC/WHO ad hoc Expert Consultation on Biotoxins in Molluscan Bivalves (Oslo, Norway, 26–30 September 2004), 31 pp. Rome, Italy: Food and Agriculture Organization. Website http://www.fao.org/es/esn/food/risk_biotoxin_en.stm (Accessed 17.01.2006).
3.
Anon. (2004). Marine Biotoxins. FAO Food and Nutrition Paper 80, 295 pp. Rome, Italy: Food and Agriculture Organization.
CombesR.D. (2003). The mouse bioassay for diarrhetic shellfish poisoning: A gross misuse of laboratory animals and of scientific methodology. ATLA31, 595–610.
6.
Anon. (1991). Council Directive 91/492/EEC of 15 July 1991 laying down the health conditions for the production and the placing on the market of live bivalve molluscs. Official Journal of the European CommunitiesL268, 1–19.
7.
Anon. (1997). Council Directive 97/79/EC of 18 December 1997 amending Directives 71/118/EEC, 72/462/EEC, 85/73/EEC, 91/67/EEC, 91/492/EEC, 91/493/EEC, 92/45/EEC and 92/118/EEC as regards the organisation of veterinary checks on products entering the Community from third countries. Official Journal of the European CommunitiesL24, 31–32.
8.
Anon. (2002). Commission Decision 2002/225/EC of 15 March 2002 laying down detailed rules for the implementation of Council Directive 91/492/EEC as regards the maximum levels and the methods of analysis of certain marine biotoxins in bivalve molluscs, echinoderms, tunicates and marine gastropods (notified under document number C(2002) 1001). Official Journal of the European CommunitiesL75, 62–63.
9.
Anon. (2002). Commission Decision 2002/226/EC of 15 March 2002 establishing special health checks for the harvesting and processing of certain bivalve molluscs with a level of amnesic shellfish poison (ASP) exceeding the limit laid down by Council Directive 91/492/EEC (notified under document number C(2002) 1009). Official Journal of the European CommunitiesL75, 65–66.
10.
Anon. (1986). Council Directive 86/609/EEC of 24 November 1986 on the approximation of laws, regulations and administrative provisions of the Member States regarding the protection of animals used for experimental and other scientific purposes. Official Journal of the European CommunitiesL358, 1–29.
11.
Anon. (1997). Council Directive 97/61/EC of 20 October 1997 amending the Annex to Directive 91/492/EEC laying down the health conditions for the production and placing on the market of live bivalve molluscs. Official Journal of the European CommunitiesL295, 35–36.
12.
Anon. (2004). Regulation (EC) No 852/2004 of the European Parliament and of the Council of 29 April 2004 on the hygiene of foodstuffs. Official Journal of the European UnionL139, 1–54.
13.
Anon. (2004). Regulation (EC) No 853/2004 of the European Parliament and of the Council of 29 April 2004 laying down specific hygiene rules for food of animal origin. Official Journal of the European UnionL139, 55–205.
14.
Anon. (2004). Regulation (EC) No 854/2004 of the European Parliament and of the Council of 29 April 2004 laying down specific rules for the organisation of official controls on products of animal origin intended for human consumption. Official Journal of the European UnionL155, 206–321.
15.
Anon. (1993). Council Decision of 14 June 1993 on reference laboratories for the monitoring of marine biotoxins. Official Journal of the European CommunitiesL166, 31–32.
Anon. (1986). European Convention for the Protection of Vertebrate Animals used for Experimental and Other Purposes. European Treaty Series No. 123, 51 pp. Strasbourg, France: Council of Europe.
19.
Anon. (2005). AOAC Official Method 959.08. Paralytic Shellfish Poison, Biological Method. In AOAC Official Methods of Analysis, 18th Edition Chapter 49: Natural Toxins (chapter ed. TrucksessM.W.), pp. 79–80. Gaithersburg, MD, USA: AOAC International.
20.
McFarrenE.F. (1959). Report on collaborative studies of the bioassay for paralytic shellfish poison. Journal of AOAC International42, 263–271.
21.
ParkD.L., AdamsW.N., GrahamS.L., & JacksonR.C. (1986). Variability of mouse bioassay for determination of paralytic shellfish poisoning toxins. Journal of AOAC International69, 547–550.
22.
MonsM.N., Van EgmondH.P., & SpeijersG.J.A. (1998). Paralytic shellfish poisoning: A review. RIVM Report 388802 005. June 1998. 60 pp. Bilthoven, The Netherlands: RIVM.
23.
LedouxM., & HallS. (2000). Proficiency testing of eight French laboratories in using the AOAC mouse bioassay for paralytic shellfish poisoning: Interlaboratory collaborative study. Journal of AOAC International83, 305–310.
24.
Anon. (2003). Marine Toxins, Pilot Study August 2003. Report Food Analysis Performance Assessment Scheme.17 pp. Sand Hutton, York, UK: Central Science Laboratory.
25.
AuneT., RamstadH., HeidenreichB., LandsverkT., WaalerT., EgaasE., & JulshamnK. (1998). Zinc accumulation in oysters giving mouse deaths in paralytic shellfish poisoning bioassay. The Journal of Shellfish Research17, 1243–1246.
26.
NagashimaY., NoguchiT., KawabataT., & HashimotoK. (1991). Dose-death time curves of paralytic shellfish poisons in ddY strain mice. Nippon Suisan Gakkaishi57, 699–704.
27.
PrakashA., MedcofJ.C., & TennantA.D. (1971). Paralytic shellfish poisoning in eastern Canada. Bulletin of the Fisheries Research Board of Canada177, 1–87.
28.
YasumotoT., OshimaY., & YamaguchiM. (1978). Occurrence of a new type of shellfish poisoning in the Tohuku District. Bulletin of the Japanese Society of Scientific Fisheries44, 1249–1255.
StabellO.B., SteffenakI., & AuneT. (1992). An evaluation of the mouse bioassay applied to extracts of “diarrhoetic” shellfish toxins. Food and Chemical Toxicology30, 139–144.
31.
YanagiT., MurataM., TorigoeK., & YasumotoT. (1989). Biological activities of semisynthetic analogs of dinophysistoxin-3, the major diarrhetic shellfish toxin. Agricultural and Biological Chemistry53, 525–529.
32.
AndersonW.A., WhelanP., RyanM., McMahonT., & JamesK.J. (2001). Risk Assessment of Azaspiracids (AZAs) in Shellfish.29 pp. Dublin, Ireland: Food Safety Authority of Ireland.
33.
DennisonN., ZuurG., PetrieJ., TurriffJ., KinnearS., & BourkeD. (2002). Pilot investigation of the use of general anaesthesia to refine the statutory mouse bioassay for paralytic shellfish poison. Abstract: Fourth World Congress on Alternatives and Animal Use in the Life Sciences, 11–15 August 2002, New Orleans, LA, USA. Website http://www.world-congress.net/2002/abstract-book/workshops/workshop-b.htm (Accessed 17.01.2006).
34.
HoltropG., PetrieJ., McElhineyJ., & DennisonN. (2005). Study to Investigate the Effect of General Anaesthesia on the Paralytic Shellfish Poison Bio-assay. Final Report Project S02012, 40 pp. London, UK: Food Standards Agency.
35.
HoltropG., PetrieJ., McElhineyJ., & DennisonN. (2006). Can general anaesthesia be used for the Paralytic Shellfish Poison bioassay?Toxicon47, 336–347.
36.
DennisonN., ZuurG., PetrieJ., TurriffJ., & KinnearS. (2002). Refinement of the mouse bioassays for detection of diarrhetic (DSP) and paralytic shellfish poisons (PSP). Poster presentation at 32nd Annual Scand-LAS Symposium, 18–21 April 2002, Gardermoen, Norway.
37.
AspT.N., LarsenS., & AuneT. (2004). Analysis of PSP toxins in Norwegian mussels by a post-column derivatization HPLC method. Toxicon43, 319–327.
38.
RossiniG.P. (2005). Functional assays in marine biotoxin detection. Toxicology207, 451–462.
39.
CembellaA.D., DoucetteG.J., & GarthwaiteI. (2004). In vitro assays for phycotoxins. In Manual on Harmful Marine Microalgae (ed. HallegraeffG.M., AndersonD.M., & CembellaA.D.), IOC Manuals and Guides No. 33, pp. 297–345. Paris, France: UNESCO.
40.
JellettJ.F., MarksL.J., StewartJ.E., DoreyM.L., Watson-WrightW., & LawrenceJ.F. (1992). Paralytic shellfish poison (saxitoxin family) bioassay: Automated endpoint determination and standardization of the in vitro tissue culture bioassay, and comparison with the standard mouse bioassay. Toxicon30, 1143–1156.
41.
LouzaoM.C., VieytesM.R., Baptista de SousaJ.M.V., LeiraF., & BotanaL.M. (2001). A fluorimetric method based on changes in membrane potential for screening paralytic shellfish toxins in mussels. Analytical Biochemistry289, 246–250.
42.
NicholsonR.A., LiG.H., BuenaventuraE., & GrahamD. (2002). A rapid and sensitive assay for paralytic shellfish poison (PSP) toxins using mouse brain synaptosomes. Toxicon40, 831–838.
43.
LouzaoM.C., VieytesJ.M., CabadoA.G., VieitesJ.M., & BotanaL.M. (2003). A fluorimetric microplate assay for the detection and quantitation of toxins causing paralytic shellfish poisoning. Chemical Research in Toxicology16, 433–438.
44.
MangerR.L., LejaL.S., LeeS.Y., HungerfordJ.M., & WekellM.M. (1993). Tetrazolium-based cell bioassay for neurotoxins active on voltage-sensitive sodium channels: semiautomated assay for saxitoxins, brevetoxins, and ciguatoxins. Analytical Biochemistry214, 190–194.
45.
MangerR.L., LejaL.S., LeeS.Y., HungerfordJ.M., HokamaY., DickeyR.W., GranadeH.R., LewisR., YasumotoT., & WekellM.M. (1995). Detection of sodium channel toxins: directed cytotoxicity assays of purified ciguatoxins, brevetoxins, saxitoxins, and seafood extracts. Journal of AOAC International78, 521–527.
46.
TrumanP., StirlingD.J., NorthcoteP., LakeJ.R., SeamerC., & HannahD.J. (2002). Determination of brevetoxins in shellfish by the neuroblastoma assay. Journal of AOAC International85, 1057–1063.
47.
LouzaoM.C., VieytesM.R., YasumotoT., & BotanaL.M. (2004). Detection of sodium channel activators by a rapid fluorimetric microplate assay. Chemical Research in Toxicology17, 572–578.
48.
SimonJ-F., & VernouxJ-P. (1994). Highly sensitive assay of okadaic acid using protein phosphatase and paranitrophenyl phosphate. Natural Toxins2, 293–301.
49.
TubaroA., FlorioC., LuxichE., SosaS., Della LoggiaR., & YasumotoT. (1996). A protein phosphatase 2A inhibition assay for a fast and sensitive assessment of okadaic acid contamination in mussels. Toxicon34, 743–752.
50.
VieytesM.R., FontalO.I., LeiraF., Baptista de SousaJ.M.V., & BotanaL.M. (1997). A fluorescent microplate assay for diarrheic shellfish toxins. Analytical Biochemistry248, 258–264.
51.
AlfonsoA., VieytesM.R., YasumotoT., & BotanaL.M. (2004). A rapid microplate fluorescence method to detect yessotoxins based on their capacity to activate phosphodiesterases. Analytical Biochemistry326, 93–99.
52.
PierottiS., MalagutiC., MilandriA., PolettiR., & RossiniG.P. (2003). Functional assay to measure yessotoxins in contaminated mussel samples. Analytical Biochemistry312, 208–216.
53.
FerrariS., CiminielloP., Dell'AversanoC., ForinoM., MalagutiC., TubaroA., PolettiR., YasumotoT., FattorussoE., & RossiniG.P. (2004). Structure-activity relationships of yessotoxins in cultured cells. Chemical Research in Toxicology17, 1251–1257.
54.
PazosM-J., AlfonsoA., VieytesM.R., YasumotoT., VieitesJ.M., & BotanaL.M. (2004). Resonant mirror biosensor detection method based on yessotoxin-phosphodiesterase interactions. Analytical Biochemistry335, 112–118.
55.
De la RosaL.A., AlfonsoA., VilarinoN., VieytesM.R., & BotanaL.M. (2001). Modulation of cytosolic calcium levels of human lymphocytes by yessotoxin, a novel marine phycotoxin. Biochemical Pharmacology61, 827–833.
56.
AlfonsoA., De la RosaL., VieytesM.R., YasumotoT., & BotanaL.M. (2003). Yessotoxin, a novel phycotoxin, activates phosphodiesterase activity. Effect of yessotoxin on cAMP levels in human lymphocytes. Biochemical Pharmacology65, 193–208.
57.
BianchiC., FatoR., AngelinA., TrombettiF., VentrellaV., BorgattiA.R., FattorussoE., CiminielloP., BernardiP., LenazG., & CastelliG.P. (2004). Yessotoxin, a shellfish biotoxin, is a potent inducer of the permeability transition in isolated mitochondria and intact cells. Biochimica et Biophysica Acta1656, 139–147.
58.
Anon. (2005). BIOTOX: Development of Cost-effective Tools for Risk Management and Traceability Systems for Marine Biotoxins in Seafood. 6th Framework project funded by the European Commission, Contract No: 514074. Website http://www.biotox.org (Accessed 17.01.2006).
59.
NicolauK.C., VyskocilS., KoftisT.V., YamadaY.M.A., LingT., ChenD.Y-K., TangW., PetrovicG., FrederickM.O., & SatakeM. (2004). Structural revision and total synthesis of Azaspiracid-1, part 1: Intelligence gathering and tentative proposal. Angewandte Chemie116, 2–8.
60.
NicolauK.C., KoftisT.V., VyskocilS., PetrovicG., LingT., YamadaY.M.A., TangW., & FrederickM.O. (2004). Structural revision and total synthesis of Azaspiracid-1, part 2: Definition of the ABCD domain and total synthesis. Angewandte Chemie43, 2–7.
61.
Anon. (2005). DetecTox: Development of an SPR-based biosensor for the detection of lipophilic phycotoxins in shellfish residues. 6th Framework project funded by the European Commission, Contract No: 514055. Website http://www.detectox.org (Accessed 17.01.2006).
62.
BadenD.G., MelinekR., SechetV., TrainerV.L., SchultzD.R., ReinK.S., TomasC.R., DelgadoJ., & HaleL. (1995). Modified immunoassays for polyether toxins: implications of biological matrixes, metabolic states, and epitope recognition. Journal of AOAC International78, 499–508.
63.
DickeyR.W., PlakasS.M., JesterE.L.E., El SaidK.R., JohannessenJ.N., FlewellingL.J., ScottP., HammondD.G., Van DolahF.M., LeighfieldT.A., Bottein DachraouiM-Y., RamsdellJ.S., PierceR.H., HenryM.S., PoliM.A., WalkerC., KurtzJ., NaarJ., BadenD.G., MusserS.M., WhiteK.D., TrumanP., HawrylukT.P., WekellM.M., StirlingD., QuilliamM.A., & LeeJ.K. (2004). Multi-laboratory study of five methods for the determination of brevetoxins in shellfish tissue extracts. In Harmful Algae 2002 (ed. SteidingerK.A., LandsbergJ.H., TomasC.R., & VargoG.A.), pp. 300–302. Proceedings of the Xth International Conference on Harmful Algae. Florida Fish and Wildlife Conservation Commission and Intergovernmental Oceanographic Commission of UNESCO. St Petersburg, FL, USA: FFWCC.
64.
Anon. (2004). Marine and Freshwater Toxins Community expresses need for modern methods. Inside Laboratory Management, September/October 2004, 9–10. Website http://www.aoac.org/marine_toxins/task_force.htm (Accessed 17.01.2006).
65.
Anon. (2003). The JELLETT RAPID TEST for ASP. Website http://www.jellett.ca/ (Accessed 17.10.2006).
66.
GarthwaiteI., RossK.M., MilesC.O., HansenR.P., FosterD., WilkinsA.L., & TowersN.R. (1998). Polyclonal antibodies to domoic acid, and their use in immunoassays for domoic acid in sea water and shellfish. Natural Toxins6, 93–104.
67.
TraynorI.M., CrooksS.R.H., & ElliottC.T. (2002). Detection of domoic acid residues in bivalve molluscs by immunobiosensor. Poster presented at the 4th International Symposium on Hormone and Veterinary Drug Residue Analysis, 4–7 June, 2002, Antwerp, Belgium.
68.
QuilliamM.A., XieM., & HardstaffW.R. (1995). Rapid extraction and cleanup for liquid chromatographic determination of domoic acid in unsalted food. Journal of AOAC International78, 543–554.
69.
Anon. (2003). ASP Direct cELISA Kit for Quantification of Domoic Acid. Product nr. A 31300400-096. Bergen, Norway: Biosense Laboratories. Website http://www.biosense.com (Accessed 17.01.2006).
70.
UdaT., & YasumotoT. (1988). Detection of diarrhetic shellfish toxins using monoclonal antibodies. Bio Industry5, 671–678.
71.
FremyJ.M., GleizesE., QuodJ.P., AmmarM., & TurquetJ. (1996). Reliable use of an ELISA test kit for DSP toxin determination in dinoflagellate cultures and shellfish. Abstract book of the IX International Symposium on Mycotoxins and Phycotoxins, 24–31 May 1996, Rome, Italy, p. 266, (ed. MiragliaM., BreraC., & OnoriR.). Rome, Italy: Istituto Superiore di Sanità.
72.
JellettJ.F., DoucetteL.I., & BellandE.R. (1998). The MIST™ shippable cell bioassay kits for PSP: An alternative to the mouse bioassay. Journal of Shellfish Research17, 1653–1655.
73.
JellettJ.F., RobertsR.L., LaycockM.V., QuilliamM.A., & BarrettR.E. (2002). Detection of paralytic shellfish poisoning (PSP) toxins in shellfish tissue using MIST Alert, a new rapid test, in parallel with the regulatory AOAC mouse bioassay. Toxicon40, 1407–1425.
74.
MackintoshF.H., GallacherS., ShanksA.M., & SmithE.A. (2002). Assessment of MIST Alert™, a commercial qualitative assay for detection of Paralytic Shellfish Poisoning toxins in bivalve molluscs. Journal of AOAC International85, 632–641.
75.
MackintoshF.H., & SmithE.A. (2002). Evaluation of MIST Alert™ rapid test kits for the detection of paralytic and amnesic shellfish poisoning toxins in shellfish. Journal of Shellfish Research21, 455–460.
76.
UsleberE., DietrichR., BürkC., SchneiderE., & MärtlbauerE. (2001). Immunoassay methods for paralytic shellfish poisoning toxins. Journal of AOAC International84, 1649–1656.
DoucetteG.J., LoganM.L., RamsdellJ.S., & Van DolahF.M. (1997). Development and preliminary validation of a microtiter plate-based receptor binding assay for paralytic shellfish poisoning toxins. Toxicon35, 625–636.
80.
RuberuS.R., LiuY-G, WongC.T., PereraS.K., & LangloisG.W. (2003). Receptor binding assay for paralytic shellfish poisoning toxins: optimization and interlaboratory comparison. Journal of AOAC International86, 737–745.
81.
Van DolahF. (2005). Receptor assays for phycotoxins. In General Referee Report on Marine and Freshwater Toxins (Hungerford, J.M., General Referee), p. 307. Journal of AOAC International88, 299–324. Website http://www.aoac.org/pdf/gr_d.pdf (Accessed 17.01.2006).
82.
Anon. (2005). BioCop: New Technologies to screen multiple chemical contaminants in food. 6th Framework integrated project funded by the European Commission, Contract No: FOOD-CT-2004-006988. Website http://www.biocop.org/. (Accessed 17.01.2006).
83.
AlfonsoC., AlfonsoA., VieytesM.R., YasumotoT., & BotanaL.M. (2005). Quantification of yessotoxin using the fluorescence polarization technique and study of the adequate extraction procedure. Analytical Biochemistry344, 266–274.
84.
Anon. (2002). Commission Decision 2002/657/EC of 12 August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results. Official Journal of the European CommunitiesL221, 8–36.
85.
McNabbP., SelwoodA.I., HollandP.T., AasenJ., AuneT., EagleshamG., HessP., IgarishiM., QuilliamM., SlatteryD., Van de RietJ., Van EgmondH., Van den TopH., & YasumotoT. (2005). A Multiresidue LC-MS method for algal toxins in shellfish: validation and interlaboratory studies. Journal of AOAC International88, 761–72.
86.
LawrenceJ.F., CharbonneauC.F., & MenardC. (1991). Liquid Chromatographic Determination of Domoic Acid in Mussels, using the AOAC Paralytic Shellfish Poison Extraction Procedure: Collaborative Study. Journal of AOAC International74, 68–72.
87.
Anon. (2005). AOAC Official Method 991.26. Domoic Acid in Mussels, Liquid Chromatographic Method. In AOAC Official Methods of Analysis, 18th Edition Chapter 49: Natural toxins (chapter ed. TrucksessM.W.), pp. 81–82. Gaithersburg, MD, USA: AOAC International.
88.
HessP., ScurfieldJ., & WellsD.E. (2006). Proficiency Testing for the quantitative determination of domoic acid, the amnesic shellfish poisoning toxin. In Proceedings of the 5th International Conference of Molluscan Shellfish Safety, 14–18 June 2004, Galway, Ireland (ed. DeeganB., ButlerC., CusackC., HenshilwoodK., HessP., KeaveneyS., McMahonT., O'CinneideM., & SilkeJ.). Galway, Ireland: Marine Institute, in press.
89.
HessP., MorrisS., StoboL.A., BrownN.A., McEvoyJ.D.G., KennedyG., YoungP.B., SlatteryD., McGovernE., McMahonT., & GallacherS. (2005). LC-UV and LC-MS methods for the determination of domoic Acid. Trends in Analytical Chemistry24, 358–367.
90.
HollandP.T., McNabbP., SelwoodA.I., & NeilT. (2003). Amnesic shellfish poisoning toxins in shellfish: estimation of uncertainty of measurement for a liquid chromatography/tandem mass spectrometry method. The Journal of AOAC International86, 1095–1103.
91.
QuilliamM.A., ThomasK., & WrightJ.L.C. (1998). Analysis of domoic acid in shellfish by thin-layer chromatography. Natural Toxins6, 147–152.
92.
LeeJ.S., YanagiT., KennaR., & YasumotoT. (1987). Fluorometric Determination of diarrhetic shellfish toxins by high performance liquid chromatography. Agricultural and Biological Chemistry51, 877–881.
93.
LawrenceJ.F., NiedzwiadekB., & MenardC. (2004). Quantitative determination of paralytic shellfish poisoning toxins in shellfish using prechromatographic oxidation and liquid chromatography with fluorescence detection: interlaboratory study. Journal of AOAC International87, 83–100.
94.
LawrenceJ.F., NiedzwiadekB., & MenardC. (2005). Quantitative determination of paralytic shellfish poisoning toxins in shellfish using prechromatographic oxidation and liquid chromatography with fluorescence detection: collaborative study. Journal of AOAC International88, 1714–1732.
95.
Anon. (2005). AOAC Official Method 2005.06. Quantitative Determination of Paralytic Shellfish Poisoning Toxins in Shellfish using Prechromatographic Oxidation and Liquid Chromatography with Fluorescence Detection.Gaithersburg, MD, USA: AOAC International.
96.
OshimaY., SuginoK., & YasumotoT. (1989). Latest advances in HPLC analysis of Paralytic Shellfish Toxins. In Mycotoxins and Phycotoxins ‘88 (ed. NatoriS., HashimotoK., & UenoY.), pp. 319–326. Amsterdam, The Netherlands: Elsevier.
97.
YasumotoT., & TakizawaA. (1997). Fluorometric measurement of yessotoxins in shellfish by high-pressure liquid chromatography. Bioscience, Biotechnology and Biochemistry61, 1775–1777.
98.
Anon. (1992). Terms and Definitions Used in Connection with Reference Materials. ISO Guide 30, 8 pp. Geneva, Switzerland: International Standardisation Organisation.
99.
MaierE.A., BoenkeA., & MériguetP. (1997). Importance of the Certified Reference Materials Programmes for the European Union. Trends in Analytical Chemistry16, 496–503.
100.
QuilliamM.A. (2006). Certified Reference Materials for Marine Biotoxins. In Proceedings of the 5th International Conference of Molluscan Shellfish Safety, 14–18 June 2004, Galway, Ireland (ed. DeeganB., ButlerC., CusackC., HenshilwoodK., HessP., KeaveneyS., McMahonT., O'CinneideM., & SilkeJ.). Galway, Ireland: Marine Institute, in press.
101.
Van EgmondH.P., MouriñoA., BurdaspalP.A., BustosJ., LegardaT., MesegoA., PaulschW.E., SalgadoC., van den TopH.J., & BoenkeA. (1998). The Certification of the Mass Fractions of Saxitoxin and dc-Saxitoxin in Two Mussel Reference Materials (CRMs 542 & 543), including the Identification of Other PSP-toxins, and a Spiking Procedure based on an Enrichment Solution (CRM 663) with a Certified Mass Concentration of Saxitoxin. EUR report 18318, 124 pp. Brussels, Belgium: European Commission, DG Science, Research and Development.
102.
Anon. (1994). European Commission, Standards, Measurements and Testing Programme. Guidelines for the Production and Certification of BCR Reference Materials (RMs). Document Doc. BCR/48/93, version 15th December 1994. 54 pp. Brussels, Belgium: European Commission.
103.
Anon. (2005). BiotoxMarin: Development of novel analytic tools for the detection of marine biotoxins. 6th Framework integrated project funded by the European Commission, Contract No: 513967. Website http://www.marine-biotoxins.org/biotoxins/related_projects.html (Accessed 17.01.2006).
104.
RehmanN., KilcoyneJ., YasumotoT., & HessP. (2006). Isolation and purification of azaspiracids. In Proceedings of the 5th International Conference of Molluscan Shellfish Safety, 14–18 June 2004, Galway, Ireland (ed. DeeganB., ButlerC., CusackC., HenshilwoodK., HessP., KeaveneyS., McMahonT., O'CinneideM., & SilkeJ.). Galway, Ireland: Marine Institute, in press.
105.
Anon. (2004). Worldwide Regulations for Mycotoxins in Food and Feed in 2003. FAO Food and Nutrition Paper 81, 180 pp. Rome, Italy: Food and Agriculture Organization.
106.
HorwitzW. (1995). Protocol for the design, conduct and interpretation of method performance studies. Pure and Applied Chemistry67, 331–343.
107.
ThompsonM., EllisonS.L.R., & WoodR. (2002). Harmonized guidelines for single-laboratory validation of methods of analysis (IUPAC Technical Report). Pure & Applied Chemistry74, 835–855.
108.
Anon. (2003). European Standard EN 14176. Foodstuffs — Determination of Domoic Acid in Mussels by HPLC.10 pp. Brussels, Belgium: European Committee for Standardization (CEN).
109.
Anon. (2004). European Standard EN 14524. Foodstuffs — Determination of Okadaic Acid in Mussels — HPLC Method with Solid Phase Extraction Clean-up, Derivatization and Fluorimetric Detection.15 pp. Brussels, Belgium: European Committee for Standardization (CEN).
110.
Anon. (2003). European Standard EN 14526. Foodstuffs — Determination of Saxitoxin and dc-Saxitoxin in Mussels — HPLC Method using Pre-column Derivatization with Peroxide or Periodate Oxidation.17 pp. Brussels, Belgium: European Committee for Standardization (CEN).
111.
Anon. (2002). European Prestandard ENV 14194. Foodstuffs — Determination of Saxitoxin and dc-Saxitoxin in Mussels — HPLC Method using Post Column Derivatization.11 pp. Brussels, Belgium: European Committee for Standardization (CEN).
112.
FernandezM.L., & Van EgmondH.P. (2004). European approaches to marine biotoxin control and harmonisation. In Harmful Algae Management and Mitigation (ed. HallS., EtheridgeS., AndersonD., KleindinstJ., ZhuM., & ZouY.). APEC Publication Number 204-MR-04.2, pp. 77–83. Singapore: Asia-Pacific Economic Cooperation.
113.
Anon. (1999). Food Analysis-Biotoxins — Criteria of Analytical Methods of Mycotoxins. CEN Report CR 13505, 8 pp. Brussels, Belgium: European Committee for Standardization (CEN).
114.
Van EgmondH.P., JonkerK.M., PoelmanM., ScherpenisseP., SternA.G., WezenbeekP., BergwerffA.A., & Van den TopH.J. (2004). Food Additives and Contaminants21, 331–340.
115.
Anon. (1991). Establishment of a European Centre for the Validation of Alternative Methods (ECVAM). Communication from the European Commission to Council and the European Parliament. SEC(91) 1794 final, 6 pp. Brussels, Belgium: European Commission.
116.
BallsM., BlaauboerB.J., FentemJ.H., BrunerL., CombesR.D., EkwallB., FielderR.J., GuillouzoA., LewisR.W., LovellD.P., ReinhardtC.A., RepettoG., SladowskiD., SpielmannH., & ZuccoF. (1995). Practical aspects of the validation of toxicity test procedures. The report and recommendations of ECVAM workshop 5. ATLA23, 129–147.
117.
CurrenR.D., SoutheeJ.A., SpielmannH., LiebschM., FentemJ.H., & BallsM. (1995). The role of prevalidation in the development, validation and acceptance of alternative methods. ATLA23, 211–217.
118.
ArcherG., BallsM., BrunerL.H., CurrenR.D., FentemJ.H., HolzhütterH-G., LiebschM., LovellD.P., & SoutheeJ.A. (1997). The validation of toxicological prediction models. ATLA25, 505–516.
119.
WorthA.P., & BallsM. (2001). The role of ECVAM in promoting the regulatory acceptance of alternative methods in the European Union. ATLA29, 135–143.
120.
BallsM., ed. (2002). Alternatives to Animal Experiments: Progress Made and Challenges Ahead. The Proceedings of the ECVAM Status Seminar 2002, held on 4–6 June 2002, in the Amphitheatre at the European Commission Joint Research Centre (JRC), Ispra, Italy. ATLA30, Suppl. 2, 1–243.
121.
HartungT., BremerS., CasatiS., CoeckeS., CorviR., FortanerS., GribaldoL., HalderM., Janusch RoiA., PrietoP., SabbioniE., WorthA., & ZuangV. (2003). ECVAM's response to the changing political environment for alternatives: consequences of the European Union chemicals and cosmetics policies. ATLA31, 473–481.
122.
EskesC., & ZuangV. eds. (2005). Alternative (Non-Animal) Methods for Cosmetics Testing: Current Status and Future Prospects. A Report Prepared in the Context of the 7th Amendment to the Cosmetics Directive for Establishing the Timetable for Phasing Out Animal Testing. ATLA33, Suppl. 1, 1–228.
123.
HartungT., BremerS., CasatiS., CoeckeS., CorviR., FortanerS., GribaldoL., HalderM., HoffmannS., Janusch RoiA., PrietoP., SabbioniE., ScottL., WorthA., & ZuangV. (2004). A modular approach to the ECVAM principles on test validity. ATLA32, 467–472.
124.
OshimaY. (1995). Post-column derivatization HPLC method for the analysis of PSP. Journal of AOAC International78, 795–799.
125.
HallS., StrichartzG., MoczydlowskiE., RavindranA., & ReichardtP.B. (1990). The saxitoxins: Sources, Chemistry, and Pharmacology. In Marine Toxins — Origin, Structure and Molecular Pharmacology (ed. HallS., & StrichartzG.), ACS Symposium Series 418, pp. 29–65. Washington, DC, USA: American Chemical Society.
126.
TachibanaK., ScheuerP.J., TsukitaniY., KikuchiH., EngenD.V., ClardyJ., GopichandY., & SchmitzF.J. (1981). Okadaic acid, a cytotoxic polyether from two marine sponges of the genus Halichondria. Journal of the American Chemical Society103, 2469–2471.
127.
MurataM., ShimataniM., SugitaniH., OshimaY., & YasumotoT. (1982). Isolation and structural elucidation of the causative toxin of the diarrhetic shellfish poisoning. Bulletin of the Japanese Society of Scientific Fisheries48, 549–552.
SatakeM., OfujiK., NaokiH., JamesK.J., FureyA., McMahonT., SilkeJ., & YasumotoT. (1998). Azaspiracid, a new marine toxin having unique spiro ring assemblies, isolated from Irish mussels. Journal of the American Chemical Society120, 9967–9968.
130.
OfujiK., SatakeM., McMahonT., SilkeJ., JamesK.J., NaokiH., OshimaY., & YasumotoT. (1999). Two analogs of azaspiracid isolated from mussels, Mytilus edulis, involved in human intoxication in Ireland. Natural Toxins7, 99–102.
131.
MurataM., KumagaiM., LeeJ.S., & YasumotoT. (1987). Isolation and structure of yessotoxin, a novel polyether compound implicated in diarrhetic shellfish poisoning. Tetrahedron Letters28, 5869–5872.
132.
AuneT., SorbyR., YasumotoT., RamstadH., & LandsverkT. (2002). Comparison of oral and intraperitoneal toxicity of yessotoxin towards mice. Toxicon40, 77–82.
133.
SatakeM., TerasawaK., KadowakiY., & YasumotoT. (1996). Relative configuration of yessotoxin and isolation of two new analogs from toxic scallop. Tetrahedron Letters37, 5955–5958.
134.
SatakeM., TubazoA., LeeJ.S., & YasumotoT. (1997). Two new analogs of yessotoxin, homoyessotoxin and 45-hydroxyhomoyessotoxin, isolated from mussels of the Addriatic Sea. Natural Toxins5, 107–110.
135.
TubaroA., SidariL., Della LoggiaR., & YasumotoT. (1998). Occurrence of yessotoxin-like toxins in phytoplankton and mussels from northern Adriatic Sea. In Harmful Algae (ed. RegueraB., BlancoJ., FernandezM.L., & WyattT.), pp. 470–472. Vigo, Spain, and Paris, France: Xunta de Galicia and Xunta de Galicia, and Intergovernmental Oceanographic Commission of UNESCO.
136.
Anon. (2004). Veterinary Medicine Guideline 3, Part I. Guideline for Metabolism Studies and for Selection of Residues for Toxicological Testing.6 pp. Rockville, MD, USA: US Food and Drug Administration. Website http://www.fda.gov/cvm/guidance/guideline3pt1.html (Accessed 17.01.2006).
137.
PleasanceS., QuilliamM.A., & MarrJ.C. (1992). Ionspray mass spectrometry of marine toxins. IV. Determination of diarrhetic shellfish poisoning toxins in mussel tissue by liquid chromatography/mass spectrometry. Rapid Communications in Mass Spectrometry6, 121–127.
138.
StoboL., LacazeJ-P., ScottA., GallacherS., SmithE., & QuilliamM.A. (2005). Liquid chromatography-mass spectrometry for analysis of lipophilic shellfish toxins described in EU Commission Decision 2002/225/EC. Journal of AOAC International88, 1371–1382.
139.
AasenJ.A.B. (2005). Identification and Quantification of Algal Toxins in Shellfish, and Characterisation of Toxin Profiles in Norwegian Mussels. PhD thesis, 61 pp. Oslo, Norway: Norwegian School of Veterinary Science, Department of Food Safety and Induction Biology.
140.
HessP., McMahonT., SlatteryD., SwordsD., DowlingG., McCarronM., ClarkeD., GibbonsW., SilkeJ., & O'CinneideM. (2003). Use of LC-MS testing to identify lipophilic toxins, to establish local trends and interspecies differences and to test the comparability of LC-MS testing with the mouse bioassay: an example from the Irish biotoxin monitoring programme 2001. In Molluscan Shellfish Safety (ed. VillalbaA., RegueraB., RomaldeJ.L., & BeirasR.), pp. 57–65. Santiago de Compostela, Spain, and Paris, France: Consellería de Pesca e Asuntos Marítimos da Xunta de Galicia and Intergovernmental Oceanographic Commission of UNESCO.
141.
HessP., NguyenL., AasenJ., KeoghM., KilcoyneJ., McCarronP., & AuneT. (2005). Tissue distribution, effects of cooking and parameters affecting the extraction of azaspiracids from mussels, Mytilus edulis, prior to analysis by liquid chromatography coupled to mass spectrometry. Toxicon46, 62–71.
142.
ThomasK., ChungS., KuJ., ReevesK., & QuilliamM.A. (2006). Analysis of PSP toxins by liquid chromatography with post column oxidation and fluorescence detection. In Proceedings of the 5th International Conference on Molluscan Shellfish Safety, 14–18 June 2004, Galway, Ireland (ed. DeeganB., ButlerC., CusackC., HenshilwoodK., HessP., KeaveneyS., McMahonT., O'CinneideM., & SilkeJ.). Galway, Ireland: Marine Institute, in press.