Questions are raised concerning the testing of botulinum toxin in animals, and the British Government's answers to Parliamentary Questions on this issue are reviewed, with an emphasis on the potential for reducing, refining and replacing the animal tests, which can involve substantial severity, and on the responsibility of the Home Office so see that the Three Rs approach is fully applied in this specific case.
Home Office (2003). Statistics of Scientific Procedures on Living Animals. Great Britain 2002, Cm 5886, 100 pp. London, UK: HMSO.
4.
SesardicD., LeungT., & Gaines DasR. (2003). Role for standards in assays of botulinum toxins: international collaborative study of three preparations of botulinum type A toxin. Biologicals31, 265–276.
5.
BallsM. (2003). What price vanity? No end in sight for the cosmetics testing saga. ATLA31, 373–375.
6.
Chief Medical Officer (2002). Advertising and prescribing of botox for ‘cosmetic use’. Chief Medical Officer's Update34 (September 2002, updated 10 October 2002), 1 p. London, UK: Medicines Control Agency.
7.
BurgenA.S.V., DickensF., & ZatmanL.J. (1949). The action of botulinum toxin on the neuromuscular junction. Journal of Physiology109, 1–24.
8.
SimpsonL.L., & TappJ.T. (1967). Actions of calcium and magnesium on the rate of onset of botulinum toxin paralysis of the rat diaphragm. International Journal of Neuropharmacology6, 485–492.
9.
SimpsonL.L. (1980). Kinetic studies on the interaction between botulinum toxin type A and the cholinergic neuromuscular junction. Journal of Pharmacology and Experimental Therapeutics212, 16–21.
10.
Home Office (1993). Report of the Animal Procedures Committee for 1992, Cm 2301, 41 pp. London, UK: HMSO.
11.
Gaines DasR.E., HeathA.B., MartinH., & SesardicD. (1999). Validation of in vitro assays for botulinum toxin: a case study. Developments in Biological Standardization101, 267–276.
12.
SesardicD. (2002). Alternatives in testing of bacterial toxins and antitoxins. Developments in Biologicals111, 101–108.
13.
SesardicD., JonesR.G.A., LeungT., AlsopT., & TierneyR. (2004). Detection of antibodies against botulinum toxins. Movement Disorders, Supplement on Basic and Therapeutic Aspects of Neurotoxins, in press.
14.
Anon. (1990). An indivisible trinity. Reduction, refinement and replacement of animal experimentation. FRAME News25, 6–7.
15.
PatonW. (1993). Man and Mouse. Animals in Medical Research, 2nd edition, 288 pp. Oxford, UK: Oxford University Press.
