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Abstract

Transepithelial electrical resistance (R_T) and the flux of fluorescein isothiocyanate (FITC) across Madin Darby canine kidney (MDCK) strain 1 cells and porcine epithelial kidney (LLC-PK1) monolayers were compared between three laboratories for a range of nephrotoxins. The precision of the REMS AutoSampler was similar to that of the Ussing chamber and the ENDOHM® technique, but superior to using chopstick electrodes, for measurements of resistance. The nephrotoxins used were selective for the proximal tubule, and in all cases, LLC-PK1 cells were more sensitive than MDCK cells. In most cases, change in R_T was a more sensitive indicator of damage than alterations in FITC flux. The REMS system provides high intra-plate precision for R_T measurements and is a higher throughput system, which is applicable to screening for nephrotoxicity in vitro.

Keywords

in vitro testing medium-throughput screening nephrotoxicity nephrotoxins transepithelial resistance transmembrane flux

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Transepithelial Resistance and Inulin Permeability as Endpoints in In Vitro Nephrotoxicity Testing

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