Sensory Nerve Endings in the Mucosa of the Epiglottis—Morphologic Investigations with Silver Impregnation,Immunohistochemistry,and Electron Microscopy

Abstract

This study was conducted in order to investigate the structure of sensory nerve endings of the human epiglottis and substance P immunoreactive nerve fibers of the canine epiglottis in relationship to physiologic functions of the larynx. The human epiglottis was observed by light microscopy (silver impregnation) and electron microscopy, and the canine epiglottis was studied by peroxidase-anti-peroxidase (PAP) immunohistochemistry. The results are summarized as follows: (1) In the membranes of the epiglottis, we observed free endings of simple or complex tree shape, corpuscle endings with glomerular patterns, and taste-bud-like structures, and (2) electron microscopic studies revealed varicosity of the terminal axon with processes that contained small, clear and large, dense cored vesicles. Substance P was observed in these structures, and it was suggested that substance P was related to perception in the larynx.

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References

1. Sasaki

Microscopic observation of the innervation of the human larynx. Tohoku Igaku Zasshi 1943;32:569–94.

2. Koizumi

On sensory innervation of larynx in dog. Tohoku J Exp Med 1953;58:199–210.

3. Koizumi

On innervation of taste-buds in larynx in dog. Tohoku J Med 1953;58:211–5.

4. Konig

Leden

The peripheral nervous system of the human larynx. Part I: the mucous membrane. Arch Otolaryngol 1961; 73: 21–34.

5. Nagai

Encapsulated sensory corpuscle in the mucosa of human vocal cord: an electron microscope study. Arch Histol Jpn 1982;45:145–53.

6. Chiba

Watanabe

Shin

Ultrastructure of the glomerular corpuscular nerve endings in the subepithelium of human epiglottis. Arch Histol Jpn 1985;48:213–21.

7. Otsuka

Konishi

Takahashi

Hypothalmic substance P as a candidate for transmitter of primary afferent neurons. Fed Proc 1975;34:1922–8.

8. Hokfelt

Johansson

Immunohistochemical distribution of substance P. In: Von Euler

Pemow B

Substance

New York: Raven Press, 1977: 117–45.

9. Hokfelt

Kellerth

Nilsson

Pemow

Experimental immunohistochemical studies on the localization and distribution of substance P in cat primary sensory neurons. Brain Res 1975;100:235–52.

10.

10. Hokfelt

Johansson

Ljungdahle

Lundberg

Schultzeeberg

Peptidergic neurons. Nature 1980;284:515–21.

11.

11. Shin

A problem of aspiration caused by recurrent laryngeal nerve palsy. In: Ishii

, ed. Recurrent laryngeal nerve palsy. Tokyo; Bunkodo, 1984: 220–9.

12.

12. Sevier

Munger

BL.

A silver method applicable to paraffin sections of formol fixed tissue. J Neuropathol Exp Neurol 1965;24:130–5.

13.

13. Stemberger

LA.

Molecular immunocytochemistry. In: Oster

Weiss

, eds. Immunocytochemistry (Foundation of immunology series). New Jersey: Prentice Hall, 1974: 172–84.

14.

14. Ninoyu

Yamashita

Kumazawa

Immunological studies of peptidergic nerve fibers in the field of otolaryngology. Part 3: distribution of peptidergic nerve fibers in the respiratory tract. Pract Otol Kyoto 1983; 76: 3229–40.

15.

15. Hisa

Sato

Fukui

Ibuta

Mizukoshi

Substance P nerve fibers in the canine larynx by PAP immunohistochemistry. Acta Otolaryngol (Stockh) 1985;100:128–33.

16.

16. Scadding

JW.

The permanent anatomical effect of neonatal capsaicin on somatosensory nerve. J Anat 1980;131:471–82.

17.

17. Suzuki

Takeda

Ultrastructure and monoamine precursor uptake of taste buds in the pharynx, nasopalatine ducts, epiglottis, and larynx of the mouse. Kaibogaku Zasshi, 1983; 58: 593–605.