(1) To characterize changes in brainstem neural activity following unilateral deafening in an animal model. (2) To compare brainstem neural activity from unilaterally deafened animals with that of normal-hearing controls.
Study Design
Prospective controlled animal study.
Setting
Vivarium and animal research facilities.
Subjects and Methods
The effect of single-sided deafness on brainstem activity was studied in Chinchilla lanigera. Animals were unilaterally deafened via gentamycin injection into the middle ear, which was verified by loss of auditory brainstem responses (ABRs). Animals underwent measurement of ABR and local field potential in the inferior colliculus.
Results
Four animals underwent chemical deafening, with 2 normal-hearing animals as controls. ABRs confirmed unilateral loss of auditory function. Deafened animals demonstrated symmetric local field potential responses that were distinctly different than the contralaterally dominated responses of the inferior colliculus seen in normal-hearing animals.
Conclusion
We successfully developed a model for unilateral deafness to investigate effects of single-sided deafness on brainstem plasticity. This preliminary investigation serves as a foundation for more comprehensive studies that will include cochlear implantation and manipulation of binaural cues, as well as functional behavioral tests.
GordonKHenkinYKralA. Asymmetric hearing during development: the aural preference syndrome and treatment options. Pediatrics. 2015;136:141-153.
2.
MooreDR. Anatomy and physiology of binaural hearing. Audiology. 1991;30:125-134.
3.
BuechnerABrendelMLesinski-SchiedatA, et al. Cochlear implantation in unilateral deaf subjects associated with ipsilateral tinnitus. Otol Neurotol. 2010;31:1381-1385.
4.
FirsztJBHoldenLKReederRMWaltzmanSBArndtS. Auditory abilities after cochlear implantation in adults with unilateral deafness: a pilot study. Otol Neurotol. 2012;33:1339-1346.
5.
HassepassFAschendorffAWesargT, et al. Unilateral deafness in children: audiologic and subjective assessment of hearing ability after cochlear implantation. Otol Neurotol. 2013;34:53-60.
6.
VlastarakosPVNazosKTavoulariE-FNikolopoulosTP. Cochlear implantation for single-sided deafness: the outcomes. An evidence-based approach. Eur Arch Otorhinolaryngol. 2014;271:2119-2126.
7.
BlascoMARedleafMI. Cochlear implantation in unilateral sudden deafness improves tinnitus and speech comprehension: meta-analysis and systematic review. Otol Neurotol. 2014;35:1426-1432.
8.
DormanMFZeitlerDCookSJ, et al. Interaural level difference cues determine sound source localization by single-sided deaf patients fit with a cochlear implant. Audiol Neurootol. 2015;20:183-188.
9.
ZeitlerDMDormanMFNataleSJLoiselleLYostWAGiffordRH. Sound source localization and speech understanding in complex listening environments by single-sided deaf listeners after cochlear implantation. Otol Neurotol. 2015;36:1467-1471.
10.
KokaKHollandNJLupoJEJenkinsHATollinDJ. Electrocochleographic and mechanical assessment of round window stimulation with an active middle ear prosthesis. Hear Res. 2010;263:128-137.
11.
ThorntonJLChevallierKMKokaKGabbardSATollinDJTollinD. Conductive hearing loss induced by experimental middle-ear effusion in a chinchilla model reveals impaired tympanic membrane-coupled ossicular chain movement. J Assoc Res Otolaryngol. 2013;14:451-464.
12.
TringaliSKokaKJenkinsHATollinDJ. Sound location modulation of electrocochleographic responses in chinchilla with single-sided deafness and fitted with an osseointegrated bone-conducting hearing prosthesis. Otol Neurotol. 2015;36:678-686.
13.
HeffnerRSHeffnerHE. Behavioral hearing range of the chinchilla. Hear Res. 1991;52:13-16.
14.
MillerJD. Audibility curve of the chinchilla. J Acoust Soc Am. 1970;48:513-523.
15.
KokaKJonesHGThorntonJLLupoJETollinDJ. Sound pressure transformations by the head and pinnae of the adult chinchilla (Chinchilla lanigera). Hear Res. 2011;272:135-147.
16.
VrettakosPADearSPSaundersJC. Middle ear structure in the chinchilla: a quantitative study. Am J Otolaryngol. 1988;9:58-67.
17.
BrowningGCGranichMS. Surgical anatomy of the temporal bone in the chinchilla. Ann Otol Rhinol Laryngol. 1978;87:875-882.
18.
JonesHGKokaKTollinDJ. Postnatal development of cochlear microphonic and compound action potentials in a precocious species, Chinchilla lanigera. J Acoust Soc Am. 2011;130:EL38-EL43.
HiltonMChenJKakigiAHirakawaHMountRJHarrisonRV. Middle ear instillation of gentamicin and streptomycin in chinchillas: electrophysiological appraisal of selective ototoxicity. Clin Otolaryngol Allied Sci. 2002;27:529-535.
21.
FerberATBenichouxVTollinDJ. Test-retest reliability of the binaural interaction component of the auditory brainstem response. Ear Hear. 2016;37:e291-e301.
22.
BeutelmannRLaumenGTollinDKlumpGM. Amplitude and phase equalization of stimuli for click evoked auditory brainstem responses. J Acoust Soc Am. 2015;137:EL71-EL77.
23.
BenichouxVBrownADAnbuhlKLTollinDJ. Representation of multidimensional stimuli: quantifying the most informative stimulus dimension from neural responses. J Neurosci. 2017;37:7332-7346.
24.
JonesHGBrownADKokaKThorntonJLTollinDJ. Sound frequency-invariant neural coding of a frequency-dependent cue to sound source location. J Neurophysiol. 2015;114:531-539.
25.
GrotheBPeckaMMcAlpineD. Mechanisms of sound localization in mammals. Physiol Rev. 2010;90:983-1012.
26.
SteckerGCHarringtonIAMiddlebrooksJC. Location coding by opponent neural populations in the auditory cortex. PLoS Biol. 2005;3:e78.
27.
MossopJEWilsonMJCasparyDMMooreDR. Down-regulation of inhibition following unilateral deafening. Hear Res. 2000;147:183-187.
28.
McAlpineDMartinRLMossopJEMooreDR. Response properties of neurons in the inferior colliculus of the monaurally deafened ferret to acoustic stimulation of the intact ear. J Neurophysiol. 1997;78:767-779.
29.
KitzesLMSempleMN. Single-unit responses in the inferior colliculus: effects of neonatal unilateral cochlear ablation. J Neurophysiol. 1985;53:1483-1500.
30.
ValeCJuizJMMooreDRSanesDH. Unilateral cochlear ablation produces greater loss of inhibition in the contralateral inferior colliculus. Eur J Neurosci. 2004;20:2133-2140.
31.
MuldersWHAMRobertsonD. Development of hyperactivity after acoustic trauma in the guinea pig inferior colliculus. Hear Res. 2013;298:104-108.
32.
WangJDingDSalviRJ. Functional reorganization in chinchilla inferior colliculus associated with chronic and acute cochlear damage. Hear Res. 2002;168:238-249.
33.
HutsonKADurhamDImigTTucciDL. Consequences of unilateral hearing loss: cortical adjustment to unilateral deprivation. Hear Res. 2008;237:19-31.
34.
JakobTFRosskothen-KuhlNIllingR-B. Induction of single-sided deafness in the newborn rat and its consequence for cochlear nucleus volume development. Hear Res. 2016;333:210-215.
35.
DongSMuldersWHAMRodgerJRobertsonD. Changes in neuronal activity and gene expression in guinea-pig auditory brainstem after unilateral partial hearing loss. Neuroscience. 2009;159:1164-1174.
36.
ArgenceMSaezISassuRVassiasIVidalPPde WaeleC. Modulation of inhibitory and excitatory synaptic transmission in rat inferior colliculus after unilateral cochleectomy: an in situ and immunofluorescence study. Neuroscience. 2006;141:1193-1207.
37.
ArgenceMVassiasIKerhuelLVidalP-Pde WaeleC. Stimulation by cochlear implant in unilaterally deaf rats reverses the decrease of inhibitory transmission in the inferior colliculus. Eur J Neurosci. 2008;28:1589-1602.
38.
BastaDGötzeRGröschelM, et al. Bilateral changes of spontaneous activity within the central auditory pathway upon chronic unilateral intracochlear electrical stimulation. Otol Neurotol. 2015;36:1759-1765.
39.
MillerAL. Effects of chronic stimulation on auditory nerve survival in ototoxically deafened animals. Hear Res. 2001;151:1-14.
40.
HardieNA. The consequences of deafness and chronic intracochlear electrical stimulation on the central auditory pathways. Clin Exp Pharmacol Physiol. 1998;25:303-309.
41.
NawazSMcNeillCGreenbergSL. Improving sound localization after cochlear implantation and auditory training for the management of single-sided deafness. Otol Neurotol. 2014;35:271-276.
42.
DoyleWJ. Eustachian tube function in the chinchilla. Arch Otolaryngol. 1985;111:305-308.
43.
RoehmPHofferMBalabanCD. Gentamicin uptake in the chinchilla inner ear. Hear Res. 2007;230:43-52.
44.
GhizAFSaltANDeMottJEHensonMMHensonOWGewaltSL. Quantitative anatomy of the round window and cochlear aqueduct in guinea pigs. Hear Res. 2001;162:105-112.
