Abstract
Objectives:
(1) Identify nascent candidate proteins producing the molecular dysregulation responsible for increased bone matrix deposition in otosclerosis compared to healthy control ossicles. (2) Evaluate the potential for active viral protein presence in otosclerosis. (3) Assess the utility of relative protein abundance quantification in bone tissue.
Methods:
Pooled bone homogenate protein samples from 50 human stapes collected from patients with otosclerosis between January 2012 and June 2013 were produced in a multistage bone isolation protocol. Control samples including ossicles acquired from nonotosclerotic patients and axial bone from fibula reconstruction procedures were prepared by identical protocols. All samples were subjected to concurrent isotope tagged relative abundance quantification (iTRAQ) proteome deep sequencing analysis.
Results:
iTRAQ analysis revealed multiple proteins with altered translation levels representing possible candidate proteins for the bony deposition involved in otosclerosis. Literature review and functional assessment of candidate proteins is undertaken.
Conclusions:
Proteomic analysis of otosclerotic bone samples provides novel potential agents in the development of otic capsule overgrowth and hearing loss. Further evaluation of these proteins may provide additional understanding of the pathophysiology of otosclerosis and develop additional treatment modalities.
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