Abstract
Objective: Prostaglandin D2 is the major prostanoid involved in infection and allergy. D prostanoid receptor (DP) and chemoattractant receptor-homologous molecule expressed on T helper type 2 (Th2) cells (CRTH2) are major prostaglandin D2 receptors. The purpose of this study is to disclose the role of prostaglandin D2 receptors in otitis media.
Method: Experimental otitis media was induced by middle ear injection of lipopolysaccharide. DP single gene-deficient (DP-/-) mice, CRTH2 single gene-deficient (CRTH2-/-) mice, and DP/CRTH2 double gene-deficient (DP-/- CRTH2-/-) mice also received lipopolysaccharide. Concentrations of cytokines in middle ear effusion were measured by ELISA.
Results: Significant production of cytokines and chemokines was detected in lipopolysaccharide-treated wild-type mice. CRTH2-/- mice also showed significant up regulation of inflammatory reactions. In contrast to CRTH2-/- mice, infiltration of inflammatory cells and expression of macrophage inflammatory protein 2, IL-1-beta, and IL-6 induced by lipopolysaccharide were reduced significantly in DP-/- mice and DP-/-CRTH2-/- mice.
Conclusion: These findings indicate that DP may play significant roles in pathogenesis of experimental otitis media.
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