The use of immunohistochemistry with anatomical and systematized classifications of nonneoplastic lesions in hematopoietic pathology of lymph nodes and spleens from rats and mice is described. Polyclonal antisera and monoclonal antibodies to leukocyte and other antigens can be used with frozen or fixed tissue sections to identify changes in cell populations in these tissues in response to tissue injury and aging. A classification for reactive lesions of lymph nodes and spleen is proposed that can be utilized for computerized pathology and toxicology data systems. These classifications are based on a systematized anatomic distribution of the lesions with the aid of immunohistochemistry. The association of some lesions with early leukemia or lymphoma of rats and mice is also discussed.
Anver MR, Cohen BJ, Lattuada CP, and Foster SJ (1982). Age-associated lesions in barrier-reared male Sprague-Dawley rats: A comparison between Hap: (SD) and CrI:COBS®CD®(SD) stocks . Exp. Aging Res. 8: 3-24.
2.
Coleman GL, Barthold SW, Osbaldiston GW, Foster SJ, and Jonas AM (1977). Pathological changes during aging in barrier-reared Fischer-344 male rats. J. Gerontol. 2: 258-278.
3.
DeLellis RA (ed) (1988). Advances in Immunohistochemistry. Raven Press, New York. 496 pp.
4.
Goodman DG, Ward JM, and Reichardt WD (1984). Splenic fibrosis and sarcomas in F-344 rats fed diets containing aniline hydrochloride, p-chloroaniline, azobenzene, o-toluidine hydrochloride, 4,4'-sulfonyl-dianiline, or D & C Red No.9J. Natl. Cancer Inst.73:265-273.
5.
Goodman DG, Ward JM, Squire RA, Chu KC, and Linhart MS (1979). Neoplastic and nonneoplastic lesions in aging F-344 rats. Toxicol. Appl. Pharmacol. 48:237-248.
6.
Grundmann E. (1991). Reaction patterns of the lymph node. In: Current Topics in Pathology, Vols. I and II, DV Ablashi (ed). Springer-Verlag, Berlin, p. 250.
7.
Iwata H., Hirouchi Y., Koike Y., Yamakawa S., Kobayashi K., Yamamato T., Kobayashi K., Inoue H., and Enomoto M. (1991). Historical control data of nonneoplastic and neoplastic lesions in F-344/DuCrj rats. J.. Toxicol. Pathol. 4: 1-24.
8.
Jones TC, Ward JM , Mohr U, and Hunt RD (eds) (1990). Hemopoietic System-Monographs on Pathology oflaboratory Animals. Springer-Verlag , Berlin. 336 pp.
9.
Kasprzak KS, Ward JM, Poirier LA, Reichardt DA, Denn AC III, and Reynolds CW (1987). Nickel-magnesium interactions in carcinogenesis: Dose effects and involvement of natural killer cells. Carcinogenesis8: 1005-1011.
10.
Konishi N., Ward JM, Reynolds CW, and Lijinsky W. (1988). Thymic T-cell lymphoma with the CD8+ (OX-8), CD4+ (W3/25) phenotype induced in F-344/ NCr rats by nitroso-2-hydroxypropylurea . Thymus12:225-237.
11.
Kroes R., Garbis-Berkvens JM, de Vries T., and van Nesselrooy HJ (1981) Histopathological profile of a Wistar rat stock including a survey of the literature. J. Gerontol. 36: 259-279.
12.
Losco P. (1992). Normal development, growth and aging of the rat spleen. In: Pathobiology of Aging Rats, U Mohr, DL Dungworth, and C Capen (eds). ILSI Press, Washington, DC, pp. 75-94.
13.
Losco P. and Harleman JH (1992). Normal development, growth and aging of the lymph node in rats. In: Pathobiology of Aging Rats, U Mohr, DL Dungworth , and C Capen (eds). ILSI Press, Washington, DC, pp. 49-73.
14.
Losco PE and Ward JM (1984). The early stage of large granular lymphocyte leukemia in the F-344 rat. Vet. Pathol. 21: 286-291.
15.
National Toxicology Program ( 1990). Toxicology and Carcinogenesis Studies of Amosite Asbestos in F-344/ NCr Rats. NTP TR 279. US Department of Health and Human Services, Public Health Service, National Institutes of Health, Research Triangle Park, North Carolina.
16.
Reynolds CW , Bere EW Jr, and Ward JM (1984). Natural killer activity in the rat. III. Characterization of transplantable large granular lymphocyte (LGL) leukemias in the F344 rat. J. Immunol. 132: 534-540.
17.
Taylor CR (ed) (1986 ). Immunomicroscopy: A Diagnostic Tool for the Surgical Pathologist . W. B Saunders, Philadelphia. 452 pp.
18.
Ward JM (1986). Leu 7 immunoreactivity in fetal olfactory epithelium and dysplastic or neoplastic olfactory lesions induced in Syrian golden hamsters by N-nitrosodiethylamine. Am. J. Pathol. 123: 371-376.
19.
Ward JM (1990). Classification of reactive lesions of lymph nodes . In: Hemopoietic Systems-Monographs on Pathology of Laboratory Animals, TC Jones, JM Ward, U Mohr, and RD Hunt (eds). Springer-Verlag , Berlin, pp. 155-161.
20.
Ward JM (1990). Classification of reactive lesions, spleen. In: Hemopoietic Systems-Monographs on Pathology of Laboratory Animals , TC Jones, JM Ward, U Mohr, and RD Hunt (eds). Springer-Verlag, Berlin, pp.220-226.
21.
Ward JM, Argilan F., and Reynolds CW (1983). Immunoperoxidase localization of large granular lymphocytes in normal tissues and lesions of athymic nude rats. J. Immunol. 131: 132-139.
22.
Ward JM, Goodman DG, Squire RA, Chu KC, and Linhart MS (1979). Neoplastic and nonneoplastic lesions in aging (C57BL/6N x C3H/HeN/F1) (B6C3F1) mice. J. Natl. Cancer Inst . 63: 849-854.
23.
Ward JM and Lynch PH (1984). Transplantability of naturally occurring benign and malignant neoplasms and age-associated nonneoplastic lesions of the aging F-344 rat as biological evidence for the histological diagnosis of neoplasms . Cancer Res. 44: 2608-2615.
24.
Ward JM and Rehm S. (1990). Applications of immunohistochemistry in rodent tumor pathology. Exp. Pathol. 40: 301-312.
25.
Ward JM, Rehm S., and Reynolds CW (1990). Tumours of the haematopoietic system. In: Pathology of Tumours in Laboratory Animals- Vol. 1 - Tumours of the Rat, VS Turusov and RD Hunt (eds). IARC, Lyon, France, pp. 625-657.
26.
Ward JM and Reynolds CW (1983). Large granular lymphocyte leukemia. A heterogeneous lymphocytic leukemia in F-344 rats. Am. J. Pathol. 111: 1-10.
27.
Ward JM and Reynolds CW (1990). Sources of antibodies and immunological reagents used for immunohistochemistry. In: Hemopoietic Systems-Monographs on Pathology of Laboratory Animals, TC Jones, JM Ward, U Mohr, and RD Hunt (eds). Springer-Verlag, Berlin , pp. 126-129.
28.
Ward JM, Reznik G., and Garner FM (1980). Prolif erative lesions of the spleen in male F-344 rats fed diets containing p-chloroaniline. Vet. Pathol. 17: 200-205.
29.
Ward JM and Reznik-Schuller H. (1980). Morphological and histochemical characteristics of pigments in aging F-344 rats. Vet. Pathol . 17: 678-685.
