Neutrophils from cord blood of healthy term infants were isolated and incubated for 30 min with varying concentrations of intravenous lipid emulsion (ILE) solution (4, 8, 20 mg/ml). In vitro assay of chemotaxis was performed after incubation for 120 min with endotoxin-activated serum (EAS). Neutrophil random motility was unchanged after ILE incubation yet chemotactic factor (EAS)-stimulated motility was significantly reduced in a dose-related pattern. (Journal of Parenteral and Enteral Nutrition14:472-473, 1990)
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References
1.
Spratt MG, Kratzing CC: Oleic acid as a depressant of reticuloendothelial activity in rats and mice . J Reticuloendothel Soc17 :135-140, 1975
2.
English D., Roloff JS, Lukens JN, et al: Intravenous lipid emulsions and human neutrophil function. J Pediatr99:913-916, 1981
3.
Wheeler JG, Boyle RJ, Abramson JS: Intralipid infusion in neonates : Effect on polymorphonuclear leukocytes function . J Pediatr Gastroenterol Nutr4:453-456, 1985
4.
Usmani SS, Harper RG, Concepcion GS, et al: In vitro effect of Intralipid on polymorphonuclear leukocyte function in the neonate. J Pediatr109:710-712, 1986
5.
Zigmond SH, Hirsch JG: Leukocyte locomotion and chemotaxis: New methods for evaluation and demonstration of a cell-derived chemotactic factor. J Exp Med137:387-410, 1973
6.
Boyden S.: The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leukocytes. J Exp Med115 :453-466, 1962
7.
Wiernik A., Jarstrand C., Julander I.: The effect of Intralipid on mononuclear and polymorphonuclear phagocytes . Am J Clin Nutr37:256-261, 1983
8.
Gallin JI, Rosenthal AS: The regulatory role of divalent cations in human granulocyte chemotaxis: Evidence for an association between calcium exchange and microtubules assembly . J Cell Biol62:594-609, 1974
9.
Jarstrand C. , Berghem L., Lahnborg G.: Human granulocyte and reticuloendothelial system function during Intralipid Infusion. JPEN2:663-670, 1978
10.
Nordenstrom J., Jarstrand C., Wiernik A.: Decreased chemotactic and random migration of leukocytes during Intralipid infusion. Am J Clin Nutr32:2416-2422, 1979
11.
Anderson DC , Hughes BJ, Smith CW: Abnormal motility of neonatal polymorphonuclear leukocytes: Relationship to impaired redistribution of surface adhesion sites by chemotactic factor of colchicine. J Clin Invest68:863-874, 1981
12.
Bruce MC, Baley JE, Medvik DA, et al: Impaired surface membrane expression of C3bi but not C3b receptors on neonate neutrophils. Pediatr Res21:306-311, 1981
