The Effect of High Lipid Diet on In Vitro Prostaglandin E2 and Thromboxane B2 Production by Splenic Macrophages

Abstract

Feeding animals a diet high in linoleic acid for 7 days had no effect on the in vitro production of PGE₂ by unstimulated macrophages. Feeding animals the high linoleic acid diet for 30 days greatly increased the production of PGE₂ by macrophages when they were unstimulated, but decreased the production of PGE₂ when they were stimulated with LPS. Feeding animals a diet high in linoleic acid for 30 days increased the production of TxB₂ by macrophages when they were unstimulated, but decreased the production of TxB₂ when they were stimulated. Normal, unstimulated splenic macrophages produced almost 80 times more TxB ₂ than PGE₂. However, when the macrophages were stimulated the ratio decreased to six or less because of a greater increase in PGE ₂ production. The high linoleic acid diet did not inhibit the antibody dependent cellular cytotoxicity of macrophages. (Journal of Parenteral and Enteral Nutrition 14:250-254, 1990)