Prophylactic and Therapeutic Effects of a Subcutaneous Injection of Sesame Oil Against Iron-Induced Acute Renal Injury in Mice

Abstract

Background: Iron intoxication causes acute nephrotoxicity in animals and humans. Sesame oil, a healthful food, increases resistance to lipid peroxidation and protects against multiple organ injury in various animal models. The authors examined the prophylactic and therapeutic effects of a subcutaneous injection of sesame oil against iron-induced acute renal injury in mice. Methods: Iron intoxication in mice was induced with an intraperitoneal injection (2 mg/kg) of ferric-nitrilotriacetate (Fe-NTA). Various doses of sesame oil (0, 1, 2, and 4 mL/kg, subcutaneously) were given immediately after (prophylactic) or 30 minutes after (therapeutic) the Fe-NTA injection. Renal injury was assessed by the rise in serum blood urea nitrogen (BUN) and creatinine (CRE) levels 3 hours after the Fe-NTA injection. Results: One hour after the Fe-NTA injection, serum BUN and CRE levels were significantly higher in Fe-NTA-treated mice than in saline-treated controls; 3 and 6 hours after the Fe-NTA injection, they were dose-dependently and significantly lower in all sesame oil–treated groups than in the group treated only with Fe-NTA and saline. Conclusion: A subcutaneous injection of sesame oil had both prophylactic and therapeutic effects against iron-induced acute renal injury in mice.

Keywords

Iron intoxication sesame oil renal injury mice

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References

Zhang

Enns

. Iron homeostasis: recently identified proteins provide insight into novel control mechanisms. J Biol Chem. 2009;284:711-715.

Milman

Pedersen

Steig

Byg

Graudal

Fenger

. Clinically overt hereditary hemochromatosis in Denmark 1948-1985: epidemiology, factors of significance for long-term survival, and causes of death in 179 patients. Ann Hematol. 2001;80:737-744.

Rasmussen

Folsom

Catellier

Tsai

Garg

Eckfeldt

. A prospective study of coronary heart disease and the hemochromatosis gene (HEF) C282Y mutation: the Atherosclerosis Risk in Communities (ARIC) study. Atherosclerosis. 2001;154:739-746.

Backman

Van Landeghem

Sikstrom

. Interaction between haemochromatosis and transferring receptor genes in different neoplastic disorders. Carcinogenesis. 1999;20:1231-1233.

Parkkila

Niemela

Savolainen

Koistinen

. HFE mutations do not account for transfusional iron overload in patients with acute myeloid leukemia. Transfusion. 2001;41:828-831.

Sayre

Perry

Atwood

Smith

. The role of metals in neurodegenerative diseases. Cell Mol Biol. 2000;46:731-741.

Berg

Gerlach

Youdim

. Brain iron pathways and their relevance to Parkinson’s disease. J Neurochem. 2001;79:225-236.

Green

Basile

Rumberger

. Transferrin and iron induce insulin resistance of glucose transport in adipocytes. Metabolism. 2006;55:1042-1045.

Walker

Jr Walker

. Effects of iron overload on the immune system. Ann Clin Lab Sci. 2000;30:354-365.

10.

Gupta

Chopra

. Effect of tocotrienols on iron-induced renal dysfunction and oxidative stress in rats. Drug Chem Toxicol. 2009;32:319-325.

11.

Centers for Medicare and Medicaid Services, Kinney

. 2005 Annual Report: ESRD Clinical Performance Measures Project. Am J Kidney Dis. 2006;48(4)(suppl 2):S1-106.

12.

Agarwal

Vasavada

Sachs

Chase

. Oxidative stress and renal injury with intravenous iron in patients with chronic kidney disease. Kidney Int. 2004;65:2279-2289.

13.

Zager

Johnson

ACM

Hanson

. Parenteral iron nephrotoxicity: potential mechanisms and consequences. Kidney Int. 2004;66:144-156.

14.

Pai

Boyd

McQuade

Harford

Norenberg

Zager

. Comparison of oxidative stress markers after intravenous administration of iron dextran, sodium ferric gluconate and iron sucrose in patients undergoing hemodialysis. Pharmacotherapy. 2007;27:343-350.

15.

Agarwal

. Proinflammatory effects of iron sucrose in chronic kidney disease. Kidney Int. 2006;69:1259-1263.

16.

Leehey

Palubiak

Chebrolu

Agarwal

. Sodium ferric gluconate causes oxidative stress but not acute renal injury in patients with chronic kidney disease: a pilot study. Nephrol Dial Transplant. 2005;20:135-140.

17.

Mimić-Oka

Savić-Radojević

Pljesa-Ercegovac

. Evaluation of oxidative stress after repeated intravenous iron supplementation. Ren Fail. 2005;27:345-351.

18.

Sugano

Akimoto

. Multifunctional gift from nature. J Chin Nutr Soc. 1993;18:1-11.

19.

Simon

Chadwick

Craker

. Herbs: An Indexed Bibliography 1971-1980. The Scientific Literature on Selected Herbs, and Aromatic and Medicinal Plants of the Temperate Zone. Hamden, CT: Archon Book; 1984. http://www.hort.purdue.edu/newcrop/med-aro/factsheets/SESAME.html . Accessed November 1, 2009.

20.

Hsu

Chen

Chein

. Sesame oil attenuates acute iron-induced lipid peroxidation-associated hepatic damage in mice. Shock. 2006;26:625-630.

21.

Awai

. Experimental iron overload using ferric nitrilotriacetate (Fe³⁺-NTA): a proposal model of the diseases in man caused by iron overload. Nippon Ketsueki Gakkai Zasshi. 1986;49:1650-1659.

22.

Hsu

Chien

Liu

. Effects of sesame oil on oxidative stress and hepatic injury after cecal ligation and puncture in rats. Shock. 2004;21:466-469.

23.

Hsu

Liu

Chu

Liu

. Protective effect of daily sesame oil supplement on gentamicin-induced renal injury in rats. Shock. 2010;33:88-92.

24.

Lucesoli

Fraga

. Oxidative stress in testes of rats subjected to chronic iron intoxication and alpha-tocopherol supplementation. Toxicology. 1999;132:179-186.

25.

Björk-Eriksson

Gunnarsson

Holmström

Nordqvist

Petruson

. Fewer problems with dry nasal mucous membranes following local use of sesame oil. Rhinology. 2000;38:200-203.

26.

Abraham

Yardley

. Erosive injury to the upper gastrointestinal tract in patients receiving iron medication: an underrecognized entity. Am J Surg Pathol. 1999;23:1241-1247.

27.

Cimino-Mathews

Broman

Westra

Illei

. Iron pill-induced tumefactive mucosal injury of the hypopharynx. Am J Surg Pathol. 2010;34: 1720-1722.

28.

Yardley

. Iron medication-associated gastric mucosal injury. Arch Pathol Lab Med. 2004;128:821-822.

29.

Zhang

Ouyang

Wieczorek

DeSoto

. Iron medication-induced gastric mucosal injury. Pathol Res Pract. 2009;205:579-581.

30.

Haig

Driman

. Iron-induced mucosal injury to the upper gastrointestinal tract. Histopathology. 2006;48:808-812.

31.

Nespor

Holley

. Patients on hemodialysis rely on nephrologists and dialysis units for maintenance health care. ASAIO J. 1992;38:M279-M281.

32.

Hage

Kanhai

Oen

van Diest

Karim

. The devastating outcome of massive subcutaneous injection of highly viscous fluids in male-to-female transsexuals. Plast Reconstr Surg. 2001;107:734-741.

33.

Wang

Zhang

Pan

. Fish oil–supplemented parenteral nutrition in severe acute pancreatitis patients and effects on immune function and infectious risk: a randomized controlled trial. Inflammation. 2009;32:304-309.

34.

Mayer

Gokorsch

Fegbeutel

. Parenteral nutrition with fish oil modulates cytokine response in patients with sepsis. Am J Respir Crit Care Med. 2003;167:1321-1328.

35.

Chopra

Tiwari

Arora

Kuhad

. Sesamol suppresses neuro-inflammatory cascade in experimental model of diabetic neuropathy. J Pain. 2010;11:950-957.

36.

Nair

. Protection of cellular DNA and membrane from γ-radiation-induced damages and enhancement in DNA repair by sesamol. Cancer Biother Radiopharm. 2010;25:629-635.

37.

Hsu

Chen

Chuang

Liu

. Sesamol delays mortality and attenuates hepatic injury after cecal ligation and puncture in rats: role of oxidative stress. Shock. 2006;25:528-532.

38.

Ghafoorunissa Hemalatha

Rao

. Sesame lignans enhance antioxidant activity of vitamin E in lipid peroxidation systems. Mol Cell Biochem. 2004;262:195-202.

39.

Ikeda

Kagaya

Kobayashi

. Dietary sesame lignans decrease lipid peroxidation in rats fed docosahexaenoic acid. J Nutr Sci Vitaminol (Tokyo). 2003;49:270-276.

40.

Yamashita

Kagaya

Higuti

Kiso

. Sesamin and alpha-tocopherol synergistically suppress lipid-peroxide in rats fed a high docosahexaenoic acid diet. Biofactors. 2000;11:11-13.

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