Abstract
Some patients with chronic fatigue syndrome say they benefit from taking vitamin supplements. We assessed functional status for the B vitamins pyridoxine, riboflavin and thiamine in 12 vitamin-untreated CFS patients and in 18 healthy controls matched for age and sex. Vitamin-dependent activities—aspartate aminotransferase (AST) for pyridoxine, glutathione reductase (GTR) for riboflavin, transketolase (TK) for thiamine—were measured in erythrocyte haemolysates before and after in-vitro addition of the relevant vitamin.
For all three enzymes basal activity (U/g Hb) was lower in CFS patients than in controls: AST 2.84 (SD0.62) vs 4.61 (1.43), P<0.001; GTR 6.13 (1.89) vs 7.42 (1.25), P<0.04; TK 0.50 (0.13) vs 0.60 (0.07), P<0.04. This was also true of activated values: AST 4.91 (0.54) vs 7.89 (2.11), P<0.001; GTR 8.29 (1.60) vs 10.0 (1.80), P<0.001; TK0.56 (0.19) vs 0.66 (0.08), P<0.07. The activation ratios, however, did not differ between the groups.
These data provide preliminary evidence of reduced functional B vitamin status, particularly of pyridoxine, in CFS patients.