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Abstract

To characterize keratin materials and assess changes due to processing, amino acid analysis (AAA) is an important tool, yielding a complex data pattern. Principal com ponents analysis is applied to AAA data taken from the literature for a wide variety of keratins from various sources as well as their main protein components. The analysis reveals that the principal components underlying the AAA spectrum mainly relate to the keratin intermediate filaments and their associated proteins on the one hand and the high glycine-tyrosine proteins on the other. Principal components analysis of amino acid analyses can thus be considered a versatile tool with a high potential to detect and assess changes in keratins due to natural or artificial causes.

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References

Etievant, P. , Schlich, P. , Bouvier , J.-C. , Symonds, P. , and Bertrand, A. , Varietal and Geographic Classification of French Red Wines in Terms of Elements, Amino Acids and Aromatic Alcohols, J. Sci. Food Agric. 45, 25-41 (1988).

Fraser, R.D.B. , MacRae, T.P. , and Rogers, G.E. , "Keratins, Their Composition, Structure and Biosynthesis," C. C. Thomas Publ., Springfield, MA, 1972.

Fraser, R.D.B. , and Mac Rae, T.P. , Molecular Structure and Mechanical Properties of Keratins, Proc. Soc. Exp. Biol. Symp. 24, 211-246 (1980).

Fratini, A. , Powell, B.C. , and Rogers, G.E. , Sequence, expression and Evolutionary Conservation of a Gene Encoding a Glycine/Tyrosine-rich Keratin-associated Protein of Hair, J. Biol. Chem. 268, 4511-4518 (1993).

Frenkel, M.J. , Gillespie, J.M. , and Reis, P.J. , Factors Influencing the Biosynthesis of the Tyrosine-rich Proteins of Wool, Aust. J. Biol. Sci. 27, 31-38 (19740.

Gillespie, J.M. , and Frenkel, M.J. , The Diversity of Keratins, Comp. Biochem. Physiol. B47, 339-346 (1974).

Graham, R.C. , "Data Analysis for the Chemical Sciences ," VCH Verlagsges mgH, Weinheim , Germany, 1993.

Kaiser, H.F. , The Application of Electronic Computers to Factor Analysis, Educ. Psychol. Measur. 20, 141-151 (1960).

Maclaren, J.A. , and Milligan, B. , "Wool Science, The Chemical Reactivity of the Wool Fibre," Science Press, Marrickville, Australia, 1981.

10.

Malinowski, E.R. , and Howery, D.G. ,, "Factor Analysis in Chemistry ," R.E. Krieger Publ. Co., Malabar, FL, 1989.

11.

Marshall, R.C. , and Gillespie, J.M. , Human Hair: Normal Composition and Chemically Induced Changes, in "Proc. 6. Int. Wool Text. Res. Conf. Pretoria " Vol. I, 1980, pp. 256-265.

12.

Marshall, R.C. , Nail, Claw, Hoof and Horn Keratin, in "Biology of the Integument, vol 2: Vertebrates," chap. 35, J. Bereiter-Hahn , A.G. Matoltsy , and K.S. Richards , Eds, Springer Verlag, Berlin, 1986.

13.

Marshall, R.C. , Orwin, D.F.G. , and Gillespie , J.M. , Structure and Biochemistry of Mammalian Hard Keratins, Electron Microsc. Rev. 4,, 47-83 (1991).

14.

Menefee, E.J. , Component Distributions in Keratins and Their Estimation from Amino Acid Analyses, J. Soc. Cosmet. Chem. 36, 17-39 (1985).

15.

Robbins, C.R. , "Chemical and Physical Behaviour of Human Hair ," 2nd ed., Springer Veriag , NY, 1988.

16.

Souren, I. , Kleiker, H. , Föhles, J. , Blankenburg. G., and Höcker, H. , Bestimmung der Antelle der Hauptproteinkomponenten von Wolle verschiedener Provenienz aus ihren Aminosäurezusammensetzungen, Melliand Textilber. 68, 480-485 (1987).

17.

StatSoft Inc., Statistica for Windows , handbook vol. 3, Tulsa, OK , 1994.

18.

Stevens, J. , "Applied Multivariate Statistics for the Social Sciences," Lawrence Erlbaum Assoc. Hillsdale, NJ, 1986. Manuscript received October 20. 1994, accepted March 28, 1995

Applying Principal Components Analysis to the Amino Acid Composition of Keratin Materials

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