Enzymatic Hydrolysis of Cotton,Linen,Ramie,and Viscose Rayon Fabrics

Abstract

Cotton, linen, ramie, and viscose rayon fabrics along with a cotton / linen blend were hydrolyzed with cellulase from Trichoderma viride. Surface fibrils were eliminated by a 6 hour treatment in all cases. The loss of fibrillar matter appeared to be the primary cause of weight loss at this stage. On prolonged treatment, cotton, linen, and viscose rayon lost weight at a faster rate than ramie and the cotton / linen blend. The fall in yam strength was progressive with increasing weight loss for cotton and viscose, while for linen and ramie it was slight initially and then increased sharply. Retention of strength after 48 hours' incubation time increased in the order viscose rayon << cotton < ramie < linen, whereas weight loss increased in the order ramie < linen < cotton < viscose rayon. X-ray crystallinity and moisture sorption of the samples did not change after the treatment, indicating that the mechanism of endwise attack of the cellulase at accessiblc cellulose chains on crystallite surfaces appeared to apply to all four fibers. The location of enzymatic attack could be monitored with a light microscope using Congo red staining in the case of cotton and linen, but not ramie or rayon. Changes in surface morphology could be followed for all the enzyme-treated fibers by scanning electron microscopy. Additionally, mechanical tests demonstrated the changes in stretchability and stiffness of the fabrics and the mobility of yams within the samples.

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References

Anonymous , Putting the Polish on Cotton Fabrics, Cotton Grower 27, 20-21 (1991).

Batra, S.K. , Other Long Vegetable Fibers, in "Handbook of Fiber Science and Technology: Vol. IV Fiber Chemistry," M. Lewin and E. M. Pearce , Eds., Marcel Dekker Inc., NY, 1985, pp. 727-807.

Bergheim, L.E.R. , Pettersson, L.G. , and Axiö-Fredriksson , U.B. , The Mechanism of Enzymatic Cellulose Degradation, Eur. J. Biochem. 61, 621-630 (1976).

Berni, R.J. , and Morris, N.M. , Infrared Spectroscopy, in "Analytical Methods for a Textile Laboratory," J. W. Weaver , Ed., AATCC, Research Triangle Park, NC , 1984, pp. 265-292.

Bertoniere, N.R. , and Zeronian, S.H. , Chemical Characterization of Cellulose, in "The Structure of Cellulose: Characterization of the Solid States," R. H. Atalla , Ed., ACS Symposium Series, vol. 340, 1987, pp. 255-271.

Bhatawdekar, S.P. , Sreenivasan, S. , Balasubramanya , R.H. , and Paralikar, K.M. , Effect of an Alkali Treatment on the Enzymolysis of Never-Dried Cotton Cellulose, Textile Res. J. 62, 290-292 (1992).

Buschle-Diller, G. , and Zeronian, S.H. , Enhancing the Reactivity and Strength of Cotton Fibers, J. Appl. Polym. Sci. 45, 967-979 (1992).

Buschle-Diller, G. , and Zeronian, S.H. , Enzymatic and Acid Hydrolysis of Cotton Cellulose after Slack and Tension Mercerization, Textile Chem. Color. (accepted for publication).

Cowling, E.B. , and Brown, W. , Cellulases and Their Applications , Adv. Chem. Ser. 95, 152-187 (1969).

10.

Finch, P. , and Roberts, J.C. , Enzymatic Degradation of Cellulose, in "Cellulose Chemistry and its Applications," T. P. Nevell and S. H. Zeronian , Eds., John Wiley & Sons, NY, 1985, pp. 312-343.

11.

Focher, B. , Marzetti, A. , Beltrame, P.L. , and Camiti, P. , Structural Features of Cellulose and Cellulose Derivatives and Their Effects on Enzymatic Hydrolysis, in "Biosynthesis and Biodegradation of Cellulose," C. H. Haigler and P. J. Weimer , Eds., Marcel Dekker, NY, 1991, pp. 293-310.

12.

Goyal, A. , Gosh, B. , and Eveleigh, D. , Characteristics of Fungal Cellulases, Bioresource Technol. 36, 37-50 (1991).

13.

Hermans, P.H. , "Physics and Chemistry of Cellulose Fibres ," Elsevier Publishing Co., Inc., NY, 1949.

14.

Kochavi, D. , Videbaek, T. , and Cedroni, D.M. , Optimizing Processing Conditions in Enzymatic Stonewashing, Am. Dyest. Rep. 79/2, 24-28 (1990).

15.

Ladisch, C.M. , Yang, Y. , Velayudhan , A. , and Ladisch, M.R. , A New Approach to the Study of Textile Properties with Liquid Chromatography, Textile Res. J. 62, 361-369 (1992).

16.

Laymann, P. , Promising New Markets Emerging for Commercial Enzymes, Chem. Eng. News, 10/1990, 17-18 (1990).

17.

Lenz, J. , Esterbauer, H. , Sattler, W. , Schurz, J. , and Wrentschur, E. , Changes in Structure and Morphology of Regenerated Cellulose Caused by Acid and Enzymatic Hydrolysis, J. Appl. Polym. Sci. 41, 1315-1326 (1990).

18.

Lundberg, J. , and Turbak, A. , Rayon, in "Kirk-Othmer Encyclopedia of Chemical Technology ," vol. 19, 3rd ed., 1982, pp. 855-880.

19.

Maycumber, S.G. , P&G Detergent Development Cheer-ed on by Cotton Inc., Daily News Rec. , no. 2, 2-3(1993).

20.

Olson, L. , A New Technology for Stoneless Stone-Washing Applications, Am. Dyest. Rep. 77/5, 19-22 (1988).

21.

Pan, N. , Zeronian, S.H. , and Ryu, H.S. , An Alternative Approach to the Objective Measurement of Fabrics, Textile Res. J. 63, 33-43 ( 1993).

22.

Pedersen, G.L. , Screws, G.A. , Jr., and Cedroni , D.M. , Biopolishing of Cellulosic Fabrics, Can. Textile J. 109, 31-35 (1992).

23.

Puri, V.P. , Effect of Crystallinity and Degree of Polymerization of Cellulose on Enzymatic Saccharification, Biotechnol. Bioeng. 26, 1219-1222 (1984).

24.

Schurz, J. , Billiani, J. , Hönel. A. , Eigner, W.D. , Janosi, A. , Hayn, M. , and Esterbauer, H. , Reaktionsmechanismus and Strukturänderungen beim enzymatischen Abbau von Cellulose durch Trichoderma-reesei-Cellulase, Acta Polym. 36, 76-80 (1985).

25.

Schurz, J. , and Hönel, A. , Enzymatische Hydrolyse von regenerierten Cellulosefasern mit Cellulase aus Trichoderma Reesei, Cell. Chem. Technol. 23, 465-476 (1989).

26.

Tomioka, M.K. , and Zeronian, S.H. , Morphological Studies on Fabrics Treated with Flame-Retardant Finishes, Textile Res. J. 44, 1-11 ( 1974).

27.

Tyndall, R.M. , Application of Cellulase Enzymes to Cotton Fabrics and Garments, Textile Chem. Color. 24/6, 23-26 ( 1992).

28.

Valtasaari, L. , The Configuration of Cellulose Dissolved in Iron-Sodiumtartrate, Makromol. Chem. 150, 117-126 (1971).

29.

Walker, L.P. , and Wilson, D.B. , Enzymatic Hydrolysis of Cellulose: An Overview , Bioresource Technol. 36, 3-14( 1991).

30.

Weimer, P.J. , Quantitative and Semiquantitative Measurements of Cellulose Biodegradation , in "Biosynthesis and Biodegradation of Cellulose," C. H. Haigler , and P. J. Weimer , Eds., Marcel Dekker, NY, 1991, pp. 263-291.

31.

Wood, T.M. , Fungal Cellulases, in "Biosynthesis and Biodegradation of Cellulose ," C. H. Haigler , and P. J. Weimer , Eds., Marcel Dekker, NY, 1991, pp. 491-525.

32.

Woodward, J. , Synergism in Cellulase Systems, Bioresource Technol. 36, 67-75 (1991).

33.

Zeronian, S.H. , Analysis of the Interaction Between Water and Textiles, in "Analytical Methods for a Textik Laboratory ," J. W. Weaver , Ed., AATCC Research Triangle Park, NC, 1984, pp. 117-128.