The development of a species-specific DNA probe for identifying goat (e.g., cashmere and mohair) fibers is described. A 54-base oligonucleotide probe hybridizes with single stranded target goat fiber DNA under conditions of low stringency. DNA isolated from as little as 100 mg of raw goat fiber can be clearly detected. Visualization of the DNA isolated from a 100% cashmere processed garment is also demonstrated.
Get full access to this article
View all access options for this article.
References
1.
AppleyardH. M., Guide to the Identification of Animal Fibres, Wira, Leeds, 1978.
2.
BautersM., Study of the Proteins Extracted by Formic Acid from Wool and Mohair, in “Proc. of the 7th Int. Wool Textile Res. Conf., Tokyo,” vol. 1, 1985, pp. 162–170.
3.
BerndtH.KalbéJ.KuropkaR.Meyer-StorkS.HöckerH., Limitations of the DNA Analysis with Respect to Fibre Identification, in “Speciality Fibres: Scientific, Technological and Economical Aspects,”vol. 106, KornerA.WortmannF. J.WortmannG.HöckerH., Eds., Schriftenreihe des Deutsches Wollforschungsinstitutes a.d. Technischen Hochschule Aachen, e.V., 1990, pp. 259–264.
4.
BucklandR. A., Sequence and Evolution of Related Bovine and Caprine Satellite DNA's, J. Mol. Biol.186, 25–30 (1985).
5.
EEC Working Party on Textile Names and Labelling-Analysis, J. Textile Inst.79, 155 (1988).
6.
HamlynP. F.NelsonG.McCarthyB. J., Applied Molecular Genetics—New Tools for Animal Fibre Identification, in “Specialty Fibres: Scientific, Technological and Economical Aspects,”vol. 106, KörnerA.WortmannF. J.WortmannG.HöckerH., Eds., Schriftenreihe des Deutsches Wollforschungsinstitutes a.d. Technischen Hochschule Aachen, e.V., 1990, pp. 249–258.
7.
HamlynP. F.NelsonG.McCarthyB. J., Wool Fibre Identification using Novel Species-Specific DNA Probes, J. Textile Inst.83, 97–103 (1992).
8.
LangleyK. D.KennedyT. A.Jr., The Identification of Speciality Fiber, Textile Res. J.51, 703–709 (1981).
9.
LeyK. F.CrewtherW. G.FlanaganG. F.JonesL. N.MarshallR. C., Release of Cuticle from Wool by Agitation in Solutions of Detergents, Aust. J. Biol. Sci.41, 163–176 (1988).
10.
MarshallR. C.GillespieJ. M.KlementV., Methods and Future Prospects for Forensic Identification of Hairs by Electrophoresis, J. Forensic Sci. Soc.25, 57–66 (1985).
11.
Meyer-StorkL. S.KalbéJ.KuropkaR.SauterS. L.HöckerH.BerndtH., Ein deutige Provenienzanalyse von Wolle und feinen Tierhaaren mit Hilfe der Erbsubstanz (DNS), Textilveredlung23, 304–307 (1988).
12.
NelsonG.HamlynP. F.McCarthyB. J., Developments in DNA-based Speciality Fibre Analysis, in “Proc. 8th Int. Wool Textile Res. Conf,” vol. II, Wool Research Organisation of New Zealand, Christchurch, New Zealand, 1990, pp. 385–401.
13.
NovakU., Structure and Properties of a Highly Repetitive DNA Sequence in Sheep, Nucleic Acid Res.12, 2343–2350 (1984).
14.
RivettD. E.LoganR. I.TuckerD. J.HudsonA. H. F., The Chemical Composition of the Cell Membrane of Speciality Animal Fibres, in “Speciality Fibres: Scientific, Technological and Economical Aspects,” (KornerA.WortmannF. J.WortmannG.HöckerH., Eds., Deutsches Wollforschungsinstitut, D-5100 Aachen, 1988, pp. 128–136.
SpeakmanP. T.HornJ. C., Distinguishing between Different Animal Fibres by Electrophoresis of Polypeptides Dissolved from Them, J. Textile Inst.78, 308–310 (1987).
17.
WortmannF. J.ArnsW., Quantitative Fiber Mixture Analysis by Scanning Electron Microscopy, Textile Res. J.56, 442–446 (1986).
18.
WortmannG.ZahnH., Investigation of Blends of Fine Animal Hairs by Two-Dimensional SDS-Polyacrylamide Gel Electrophoresis, in “Proc. 7th Int. Wool Textile Res. Conf., Tokyo,” vol. II, 1985, pp. 195–204.
