The sterol fraction of the internal lipids of wool has been separated by HPLC into two major components, cholesterol and its precursor, desmosterol (cholesta-5,24-dien- 3β-ol). The total of extractable sterols varied significantly between the six samples examined, representing wool from different breeds of sheep and from different climatic zones. However, the ratio of desmosterol to cholesterol remained relatively constant.
Get full access to this article
View all access options for this article.
References
1.
Baumann, H., Kulkarni, V.G., and Bobrowski, A., Der Einflub Der Morphologie Der Wolle Auf Die Topochemische Reaktion Eines Vinylsulfonfarbstoffes, in "Proc. 6th Int. Wool Text. Res. Conf., Pretoria," vol. II, 1980, pp. 519-536.
2.
Baumann, H., and Möchel, L., Wollgelatine Isolierung und Untersuchung von Heisswasserlöslichen Wollproteinbestandteilen , in "Proc. 5th Int. Wool Text. Res. Conf., Aachen ," vol. II, 1975, pp. 299-312.
3.
Baumann, H., and Setiawan, L.D., Topochemistry of Dyeing and Chemical Processing of Wool, in "Proc. 7th Int. Wool Text. Res. Conf., Tokyo," vol. V, 1985, pp. 1-13.
4.
Birkby, C.S. , Wertz, P.W., and Downing, D.T., The PolarLipids from Keratinized Tissues of Some Vertebrates, Comp. Biochem. Physiol.738, 239-242 (1982).
5.
Clayton, R.B. , Nelson, A.N., and Frantz, I.D., TheSkin Sterols of Normal and Triparanol-treated Rats, J. Lipid Res.4, 166-178 (1963).
6.
Crabtree, H.E. , Nicholls, P., and Truter, E.V., Identification of the Internal Lipids of the Wool Fibre by Thin-layer Chromatography, Proc. Anal. Div. Chem. Soc.16, 235(1979).
7.
Dahl, C.E., Dahl, J.S., and Bloch, K., Effect of AlkylSubstituted Precursors of Cholesterol on Artificial and Natural Membranes and on the Viability of Mycoplasma capricolum, Biochem.19, 1462-1467 (1980).
8.
Doddrell, D.M. , Pegg, D.T., and Bendall, M.R., Distortionless Enhancement ofNMR Signals by Polarization Transfer, J. Magn. Reson.48, 323-327 (1982).
9.
Elias, P.M., Epidermal Lipids, Membranes, and Keratinization, Int. J. Dermatol.20, 1-19 (1981).
10.
Holt, L.A., and Saunders, J.A., A Fluorescence Microscopy Study of the Diffusion of Some Nonionic Compounds into Wool from Solvent Mixtures, Textile Res. J.56, 415-418 (1986).
11.
Joseph-Nathan, P., Mejta, G., and Abrama-Bruno , D., 13C NMR Assignment of the Side-Chain Methyls of C27 Steroids, J. Am. Chem. Soc.101, 1289-1291 (1979).
12.
Leeder, J.D. , Bishop, D.G., and Jones, L.N., Internal Lipids of Wool Fibers, Textile Res. J.53, 402-407 (1983).
13.
Leeder, J.D., Rippon, J.A., Rothery, F.E., and Staple-ton, I.W. , Use of the Transmission Electron Microscope to Study Dyeing and Diffusion Processes, in "Proc. 7th Int. Wool Text. Res. Conf., Tokyo," vol. V, 1985, pp. 99-109.
14.
Leikol, E., Nieminen, E., and Teppo, A.M., NewSialic Acid-containing Sulfolipid: "Ungulic Acid", J. Lipid Res.10, 440-444 (1969).
15.
Massey, I.J. , and Djerassi, C., Structural andStereochemical Applications of Mass Spectrometry in the Marine Sterol Field, J. Org. Chem.44, 2448-2456 (1979).
16.
Orwin, D.G.F. , and Thomson, R.W., An Ultrastructure Study of the Membranes of Keratinizing Wool Follicle Cells, J. Cell Sci.11, 205-219 (1972).
17.
Orwin, D.F.G. , and Thomson, R.W., The Electron Microscopy of Two Types of Fibre Damage and its Relationship to Some Cell Components, in "Proc. 5th Int. Wool Text. Res. Conf., Aachen," vol. II, 1975, pp. 173-183.
18.
Pegg, D.T., Doddrell, D.M., and Bendall, M.R., Correspondence Between INEPT and DEPT Pulse Sequences for Coupled Spin-half Nuclei, J. Magn. Reson.51, 264-269 (1983).
19.
Sakamoto, O. , Fujinuma, Y., and Ozowa, T., The Studies on the Chemical Composition of Internal Human Hair Lipid, J. Am. Oil Chem. Soc.54, 143A (1977).
20.
Schwan, A., and Zahn, H., Isolation and Properties of Cell Membrane Complexes in Wool and Hair, in "Proc. 6th Int. Wool Text. Res. Conf., Pretoria," vol. II, 1980, pp. 29-39.
21.
Shaw, D.A., The Extraction, Quantitation and Nature of Hair Lipid, Int. J. Cosmet. Sci. 1, 291-302 (1979).
22.
Singleton, J.A., and Pattee, H.W., Optimization of Parameters for the Analysis of Triglyceride by Reverse Phase HPLC Using a UV Detector at 210 nm, J. Am. Oil Chem. Soc.61, 761-767 (1984).
23.
Svoboda, J.A. , Kaplanis, J.N., Robbins, W.E., and Thompson , M.J., RecentDevelopments in Insect Steroid Metabolism , Ann. Rev. Entomol.20, 205-220 (1975).
24.
Swift, J.A. , and Holmes, A.W., Degradation of Human Hair by Papain, Textile Res. J.35, 1014 (1965).
25.
Zahn, H., Fibers in Macromolecular Chemistry, Lenzinger Ber.42, 1-16 (1977).
26.
Zahn, H., Wool is Not Keratin Only, in "Proc. 6th Int. Wool Text. Res. Conf., Pretoria," Addendum vol. I, 1980.