A number of combinations of staining and embedding procedures have been investigated to determine which are the most suitable for TEM studies of oxidatively shrinkproofed wool fibers. The site of attack on the wool fibers by the KMnO4/salt shrinkproofing treatment, as applied in the laboratory, was found to be the scale tips. The histochemistry of the various staining reactions is discussed.
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References
1.
Anderson, C.A. and Leeder, J.D., Residual Lipid Contaminants in Processed Wool; the Composition of the Free Fatty Acids in Wool Fabric , Textile Res. J.35, 416-421 (1965).
2.
Andrews, M.W. , Inglis, A.S., Rothery , F.E., and Williams , V.A., Wool Shrinkproofing Studies, Part III: Fiber Modification in Neutral Potassium Permanganate-Salt Treatments, Textile Res. J.33, 705-711 (1963).
3.
CSIRO, Division of Textile Industry, "Shrinkproofing of Woven Wool Fabrics," Report G8, 1959 .
4.
Leduc, E.H. and Bernhard, W., Ultrastructural Cytochemistry, Enzyme and Acid Hydrolysis of Nucleic Acids and Proteins, J. Biophys. Biochem. Cytol.10, 437-445 (1961).
5.
Luft, J.H., Improvements in Epoxy Resin Embedding Methods, J. Biophys. Biochem. Cytol. 9, 409 (1961).
6.
Maclaren, J.A. , Quantitative Reduction and Alkylation of Wool , Textile Res. J.41, 713 (1971).
7.
Makinson, K.R. , Some New Observations on the Effects of Mild Shrinkproofing Treatments on Wool Fibers, Textile Res. J.38, 831-843 ( 1968).
8.
Makinson, K.R. , The Integration of Various Observations on the Mechanisms of "Degradative" Anti-Felting Treatments, J. Textile Inst.64, 228-230 (1973).
9.
Makinson, K.R. , A Convenient Method For Preparing the Cell-Membrane Network of the Wool Fiber, and a Note on the Effect of Ethanol on the Membranes in the Intact Fiber, TextileRes. J.46, 360-366 (1976).
10.
Makinson, K.R. , Bilateral Differentiation in the Cuticle of Merino Wool Fibers, Textile Res. J.48, 598-603 (1978).
11.
Makinson, K.R. , "Shrinkproofing of Wool," Marcel Dekker, New York, 1979, pp. 22-23.
12.
Stäubli, W. , A New Embedding Technique for Electronmicroscopy, Combining a Water-Soluble Epoxy Resin (Durcupan) with Water-Insoluble Araldite , J. Cell Biol.16, 197-201 (1963).
