The cotton-gin trash, bract, sisal, and jute antigens were separated into two fractions by Sephadex G-75 column chromatography. The first appeared in the chromatogram as a small, fast-moving fraction, and the second as a large, slow- moving one. Both fractions absorb uv light at 254 nm. Earlier work on the partially- purified antigens showed two bands by gel electrophoresis: a large, slow-moving band that stained heavily for carbohydrate but weakly or not at all for protein, and a small, fast-moving band that stained heavily for protein but less for carbohydrate, indicating the presence of a glycoprotein. The two fractions separated by column chromatogra phy were tested by immunodiffusion for antigenicity. The small, fast-moving fraction is immunologically active against cotton dust antibodies, but the large, slow-moving glycoprotein separated in this procedure is not.
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References
1.
Antweiler, H. , TheHistamine Releasing Capacity of Cotton Dust Extracts Inhaled by Guinea Pigs , Ann. N.Y. Acad. Sci.221, 136-140 (1974).
2.
Bouhuys, A., Response to Inhaled Histamine in Brochial Asthma and in Byssinosis , Am. Rev. Respir. Dis.95, 89-93 (1967).
3.
Bouhuys, A., Barbero, C.A., Lindell, S.E., Roach, S.A., and Schilling, R.S.F. , Byssinosis in Hemp Workers, Arch. Environ. Health14, 533-544 (1967).
4.
Bouhuys, A., Hartogensis, F., and Korfage, H.J.H., Byssinosis Prevalence and Flax Processing, Brit. J. Industr. Med.20, 320-323 (1963).
5.
E1 Batawi, M.A. , Byssinosis in the Cotton Industry of Egypt , Brit. J. Industr. Med.19, 126-130 ( 1962).
6.
Evans, E. and Nicholls, P.J., Preliminary Characterization of the Histamine Releasing Activity of Cotton Dust, J. Pharm. Pharmcol.26, 115P-116P (1974).
7.
Fisher, J.J., "Relation of Airborne Bacteria to Endotoxin Levels in Cotton Mills ," Proceedings, 1980 Beltwide Cotton Production Research Conferences and 4th Cotton Dust Session, St. Louis, Mo., 1980, pp. 29-30.
8.
Gandevia, B. and Milne, J., Ventilatory Capacity on Exposure to Jute Dust and the Relevance of Productive Cough and Smoking to the Response , Brit. J. Industr. Med.22, 187-195 (1965).
9.
Gilson, J.C. , Stott, H., Hopwood , B.E.C., Roach, S.A., McKerrow, C.B., and Schilling , R.S.F., Byssinosis: The Acute Effect on Ventilatory Capacity of Dusts in Cotton Ginneries, Cotton, Sisal and Jute Mills, Brit. J. Industr. Med.19, 9-18 (1962).
10.
Mair, A., Smith, D.H., Wilson, W.A., and Lockhart, W., Dust Diseases in Dundee Textile Workers, Brit. J. Industr. Med.17, 272-278 (1960).
11.
McKerrow, C.B. , Gilson, J.C., Schilling , R.S.F., and Skidmore , J.W., Respiratory Function and Symptoms in Rope Makers, Brit. J. Industr. Med.22, 204-209 (1965).
12.
Massoud, A. and Taylor, G., Byssinosis : Antibody to Cotton Antigens in Normal Subjects and in Cotton Cardroom Workers , Lancet2, 607-610 ( 1964).
13.
Morey, P.R., Sasser, P.E., Bethea, R.M., and Hersh, S.P., "Use of Dyed Leaf in Studies on the Origin of Cotton Dust," Proceedings of a Special Session on Cotton Dust, Beltwide Cotton Production-Mechanization Conference, Dallas, Tex., 1978, pp. 97-101.
14.
Mustafa, K.Y. , Lakha, A.S., Milla , M.H., and Dahoma, U., Byssinosis, Respiratory Symptoms and Spirometric Lung Function Tests in Tanzanian Sisal Workers, Brit. J. Industr. Med.35, 123-128 (1978).
15.
Oehling, A. , Gonzalez de la Reguera , I., and Vines Rueda, J.J., A Contribution to the Allergic Ethiopathogenity of Byssinosis, Respiration29, 155-160 (1972).
16.
Popa, V., Gavrilescu, N., Preda, N., Teculescu, D., Plecias, M., and Cirstea, M., An Investigation of Allergy in Byssinosis: Sensitization to Cotton, Hemp, Flax and Jute Antigens, Brit. J. Industr. Med.26, 101-108 (1969).
17.
Rylander, R. and Snella, M-C., "Effects of Subchronic Endotoxin Exposure," Proceedings, 1980 Beltwide Cotton Production Research Conferences and 4th Cotton Dust Session, St. Louis, Mo., 1980, pp. 26-28.
18.
Schilling, R.S.F. , Byssinosis in Cotton and Other Textile Workers , Lancet2, 319-325 (1956).
19.
Schilling, R.S.F., "Byssinosis—Epidemiology and Recent Developments," Proceedings, IVth International Pneumoconiosis Conference, Bucharest, Rumania, 1971, pp. 433-443.
20.
Sekul, A.A. and Ory, R.L., "Immunological Responses of Rabbits to Cotton Dust and Bract," Proceedings, 1978 Beltwide Cotton Production-Mechanization Conference and Special Sessions: Cotton Dust, Dallas, Tex., 1978, pp. 120-122.
21.
Sekul, A.A. and Ory, R.L., Specific Antibody Response of Rabbits to Water Extracts of Cotton Dust and Bract, Textile Res. J.49, 523-525 (1979).
22.
Sekul, A. A. and Ory, R. L., Unpublished results, 1978.
23.
Siddhu, C.M.S. , Nath, Kedar, and Mehrotra . R.K., Byssinosis Amongst Cotton and Jute Workers in Kanpur, Ind. J. Med. Res.54, 980-994 (1966).
