Partial amino acid sequences have been determined for three type I and two type II helical segments constituting a major part of the helical fragments obtained by partial proteolysis of S-carboxymethylkerateine from wool. There were few differences in the N-terminal sequences of the segments of the same type, as compared with about 70% difference between different segment types.
Get full access to this article
View all access options for this article.
References
1.
Chou, P.Y. and Fasman, G.D., Prediction of Protein Conformation, Biochemistry13, 222245 (1974).
2.
Crewther, W.G. and Dowling, L.M., The Preparation and Properties of Large Peptides from the Helical Regions of the Low-Sulphur Proteins of Wool, Appl. Polym. Symp., No. 18, 1-20 (1971).
3.
Crewther, W.G. , Fraser, R.D.B., Lennox, F.G.L., and Lindley , H.L., The Chemistry of Keratins, Adv. Protein Chem.20, 191-346 (1965).
4.
DayhofT, M.O., Eck, R.V., and Park, C.M., "Atlas of- Protein Sequence and Structure," Vol. 5, National Biomedical Research Foundation, Silver Spring, Maryland, 1972, pp. 95 99.
5.
Dirnhuher, P. and Schutz, F., The Isomeric Transformation of Urea into Ammonium Cyanate in Aqueous Solutions, Biochem. J.42, 628-632 (1948).
6.
Dowling, L.M. and Crewther, W.G., Ditribution of Two Types of α-Helical Units in the Low-Sulphur Protein Chains from α-Keratin, Proc. Aust. Biochem. Soc.5, 3 (1972).
7.
Edman, P. and Begg, G., A Protein Sequenator, Eur. J. Biochem.1, 80-91 (1967).,
8.
Fraser, R.D.B. and MacRac, T.P., "Conformation in Fibrous Protein," Academic Press , New York, 1973.
9.
Gough, K.H., Inglis, A.S., and Crewther, W.G., Amino Acid Sequence of a Type I Segment. Biochem. J., in preparation (1977):
10.
Inglis, A.S. and Nicholls, P.W., Identification of Phenylthiohydantoins of Amino Acids by Thin-Layer Chromatography, J. Chromatogr. , 79, 344-346 (1973).
11.
Inglis, A.S. , Nicholls, P.W., and Roxburgh , C.M., Acid ' Hydrolysis of Phenylthiohydantoins of Amino Acids, Aust. J. Biol. Sci.24, 1247-1250 (1971).
12.
Jones, L.N. , The Isolation and Characterization of α-Keratin Microfibrils, Biochim. Biophys. Acta412, 91-98 (1975).
13.
Robson, B. and Suzuki, E., Conformation Properties of Amino Acid Residues in Globular Proteins , J. Mol. Biol.107, 327-356 (1976).
14.
Warner, R.C. , The Kinetics of the Hydrolysis of Urea and of Arginine, J. Biol. Chem.142, 705-723 (1942).
