A new buffer system for the ion-exchange resin chromatography of amino acids (using a Technicon Autoanalyzer) is suggested. In this system all the amino acids from the acid hydrolysate of wool are resolved satisfactorily without any loss in color yield and using only three aqueous buffers to form a gradient. No methanol is used in stock buffer preparation or during the regeneration of the resin column, so that the separation method becomes less expensive, avoids spurious absorption of methanol by resin, and prevents overlapping of certain amino acid peaks on the chromatogram.
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