A method for the characterization of the products of the reaction between wool and formaldehyde is described. The method involves the use of a mild procedure to hydrolyze the formaldehyde-treated wool using digestion with proteolytic enzymes, and subsequent chromatographic separation of the resultant amino acids. Lysine and glutamine side chains have been identified as two of the sites of reaction.
Alexander, P. , Hudson, R.F., and Earland, C., "Wool: Its Chemistry and Physics," London, Chapman and Hall, pp. 327-331, 1963.
2.
Dawson, D.J. and Mauritzen, C.M., Interaction of Formaldehyde with Tetanus Toxin, Australian J. Biol. Sci.22, 1217-1227 (1969).
3.
Fraenkel-Conrat, H., Cooper, M., and Olcott, H.S., The Reaction of Formaldehyde with Proteins , J. Amer. Chem. Soc.67, 950-954 (1945).
4.
Fraenket-Conrat, H., and Olcott, H.S., Reaction of Formaldehyde with Proteins , J. Biol. Chem.174, 827-843 (1948).
5.
Haworth, R.D. , MacGillivray, R., and Peacock, D.H., The Hydrolysis of Formaldehyde-hardened Proteins by Enzymes, J. Chem. Soc., 2981-2987 (1952 ).
6.
Holt, L.A., Roxburgh, C.M., and Milligan, B., Aspartic acid, Asparagine, Glutamic acid and Glutamine Contents of Wool and two Derived Protein Fractions, Australian J. Biol. Sci.24, 509-514 (1971).
7.
Neuberger, A. and Sanger, F., The Availability of the Acetyl Derivatives of Lysine for Growth, Biochem. J.37, 515-518 (1943).
8.
O'Donnell, I.J. and Thompson, E.O.P., Studies on Reduced Wool, Australian J. Biol. Sci.17, 973-989 (1964).
9.
Pulvermacher, G., Ueber einige Reactionen des Trithioformaldehyds und Formaldehyds, Ber. Deut. Chem. Ges.25, 307-311 (1892).
10.
Walker, J.F., "Formaldehyde," New York, Reinhold, pp. 399 -404, 1964.
