A reinvestigation of a fraction of the high-sulfur proteins SCMKB was made. The proteins were successively fractionated on an electrophoretic, molecular-size, and chromatographic basis. This work resulted in the isolation of a homogeneous protein SCMKB-IIIB2 which has a molecular weight of 11,260, no free amino-terminal group, and contains S-carboxymethyl cysteine as carboxy-terminal residue.
BraunV.SchroederW. A., Reinvestigation of the Hydrazinolytic Procedure for the Determination of Carbon Terminal Amino Acids, Arch Biochem. Biophys.118, 241–252 (1967).
2.
FlodinP.KillanderJ., Fractionation of Human-Serum Proteins by Gel Filtration, Biochim. Biophys. Acta63, 403–410 (1962).
3.
GillespieJ. M., The Isolation and Properties of Some Soluble Proteins from Wool. Pt. IV: The Isolation of the High-Sulfur Protein SCMKB1, Australian J. Biol. Sci.15, 572–588 (1962).
4.
GillespieJ. M., Isolation and Properties of Some Soluble Proteins from Wool. VII, Australian J. Biol. Sci.16, 259–280 (1963).
5.
GillespieJ. M.HarrapB. S., The Isolation and Properties of Some Soluble Proteins from Wool. Pt. VI: The Physiochemical Properties of SCMKB2, Australian J. Biol. Sci.16, 252–258 (1963).
6.
HillR. L.SchmidtW. R., Complete Enzymic Hydrolysis of Proteins, J. Biol. Chem.237, 389–396 (1962).
7.
JoubertF. J.BurnsM. A. C., Fractionation of the High-Sulfur Proteins of Reduced Merino Wool, J. S. African Chem. Inst.20, 161–173 (1967).
8.
JoubertF. J.De JagerP. J.SwartL. S., “Symposium on Fibrous Proteins,” Australia, 1967. Ed. Crewther, W. G., Butterworths, Australia, 1968, p. 343.
9.
PorathJ.LindnerE. B.JerstedtS., Migration-Elution of Zones in an Electrophoresis Column, Nature182, 744–745 (1958).
10.
SangerF.ThompsonE. O. P., Halogenation of Tyrosine During Acid Hydrolysis, Biochim. Biophys. Acta71, 468–471 (1963).
11.
SoberH. A.GutterF. J.WyckoffM. M.PetersonE. A., Chromatography of Proteins. II: Fractionation of Serum Protein on Anion-Exchange Cellulose, J. Am. Chem. Soc.78, 756–763 (1956).
12.
YphantisD. A., Rapid Determination of Molecular Weights of Peptides and Proteins, Ann. N. Y. Acad. Sci.88, 586–601 (1960).